Real-time cellular analysis for skin toxicity testing of nanozeolites by using human cell culture model

Abstract

Nanoparticles using in human must be fully assessed for their safety. Nanozeolites, an aluminosilicate crystalline with a 3-dimensioned porous structure, is considered a good absorbent and ion exchanger which might be applied for use as a reservoir to entrap active ingredients of cosmetic products. Aim of this study was to investigate cytotoxicity of nanozeolites on human epidermoid carcinoma (A431) and human forehead fibroblast cell (HFF). Skin toxicity test of nanozeolites was performed by using real-time xCELLigence impedance analysis. In this study nanozeolites was ultrasonically dispersed to obtain clear solution with various concentrations at 0.05, 0.1, 0.2, 0.3, 0.4 and 0.5 % (w/v) and tested with A431 cells and HFF cells. Viable cells were monitored during the period of 24-h exposure by using the xCELLigence. Following real-time analysis, the half maximal inhibitory concentration (IC50) determined based on dose-response curves derived by xCELLigence measurement was found to be 0.08% (w/v). Results demonstrated the application of real time analysis for cellular viability and showed the IC50 value of nanozeolites at 0.08 % (w/v). This leads to a safety zone determination of using nanozeolites as a particle carrier for pharmaceutical and/or cosmetic products.