Subacute Toxicity Test of Galangin and Ceftazidime in Mice

Abstract

Galangin is one of several naturally-occuring flavonols, which is a member of flavonoid family. It is important constituents of normal human diet and also has various pharmacological properties. However, in vivo toxicity test of galangin have not been studied. Thus, the purpose of this research was to investigate the toxicity of galangin alone and in combination with ceftazidime antibiotic in vivo. The mice were administered intraperitoneally (i.p.) with galangin alone at 10 and 20 mg/kg BW/day (group1 and group 2 respectively), galangin in combination with ceftazidime at 10 mg/kg BW/day galangin plus 160 mg/kg BW/day ceftazidime (group3), and 20 mg/kg BW/day galangin plus 320 mg/kg BW/day ceftazidime (group4), twice daily for 14 days. At the end of the experiments, blood and the selected main organs were collected for haematological and histological analysis. There was no significant difference in either the growth rate of total body weight or the relative weight of the selected main body organs of mice treated with all doses of galangin alone or in combination with ceftazidime, when compared with the control. The histology of all the liver, spleen, heart, kidney and stomach exhibited a normal appearance in comparison with the control. The blood chemistry marker assayed showed no significant difference (p<0.05) in all treated groups except for the decrease in haematocrit level which were significantly difference (p<0.05) in all treated groups.