Effect of Nutri-Jelly Matrix on Cytotoxicity of Phenethyl Isothiocyanate in Oral Cancer Cells

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Patumrat Amornsil
Thunnalin Winuprasith
Dunyaporn Trachootham

Abstract

Phenethyl isothiocyanate (PEITC) is a phytochemical in cruciferous vegetable which has selective anti-cancer effects. Nutri-PEITC Jelly has been developed for cancer patients with chewing and swallowing difficulties by addition of PEITC into Nutri-jelly. However, it is unclear if Nutri-jelly matrix affects activity of PEITC. Therefore, this study was aimed to investigate the effect of Nutri-jelly matrix on cellular uptake and cytotoxicity of PEITC against oral cancer cells (CAL-27) by comparison between PEITC in Nutri-Jelly and pure PEITC. The results from MTT assay showed that concentration causing 50% cell viability (IC50) of PEITC in Nutri-Jelly was lower than that of pure PEITC, when exposed for 72 hours. In contrast, after 24 hours incubation cell death analysis using Annexin V- PI showed that PEITC in Nutri-jelly induced less percentage of cell death than that of pure PEITC. Consistently, after 1 hour of exposure PEITC in Nutri-jelly was up taken into the cancer cells less than that of pure PEITC. The findings suggest that Nutri-jelly matrix affect cellular uptake and cytotoxicity of PEITC. Future studies under oral simulated condition are warranted.

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How to Cite
Amornsil, P. ., Winuprasith, T. ., & Trachootham, D. . (2020). Effect of Nutri-Jelly Matrix on Cytotoxicity of Phenethyl Isothiocyanate in Oral Cancer Cells. Thai Journal of Toxicology, 35(1), 50–62. Retrieved from https://li01.tci-thaijo.org/index.php/ThaiJToxicol/article/view/245024
Section
Research Articles
Author Biographies

Patumrat Amornsil

Master Program in Toxicology and Nutrition for Food Safety, Mahidol University, Thailand

Thunnalin Winuprasith

Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand

Dunyaporn Trachootham

Institute of Nutrition, Mahidol University, 999 Phutthamonthon 4 Rd., Salaya, Phutthamonthon, Nakhon Pathom, 73170, Thailand
Tel. 66-2-800-2380 ext. 326, Fax. 66-2-441- 9344
Email: dunyaporn.tra@mahidol.ac.th; dunyaporn.tra@mahidol.edu