Alterations of Serum CCR5 and Carbohydrate Metabolic Proteomics in Hypertri-glyceridemia

Abstract

Hypertriglyceridemia, obesity, and insulin resistance are recognized as the predictive markers for coronary artery disease risk. However, until now their specific etiologies and mechanism of pathogenesis remain unclear. We therefore conducted a proteome association study to identify protein profiles between control and hypertriglyceridemia groups. There were 2 study groups; Control group (N=5) was healthy male and hypertriglyceridemia group were divided into 3 subgroups (N=6/subgroup) based on plasma triglyceride levels; (1) borderline TG level (150–199 mg/dL); (2) high TG level (200–499 mg/dL); and (3) very high TG level    (≥ 500 mg/dL). LC-MS/MS-based serum proteomics analysis revealed chemokine and cytokine signaling pathway (6.5%), the integrin signaling pathway (6.1%), and Wnt signaling pathway (4.2%). Protein-protein interaction as well as molecular network related to triglyceride were observed. Relative fold change of serum CCR5 was found with possible association with dyslipidemia, obesity, insulin resistance, and inflammation. Overall findings indicated that relative abundance of the proteome varied with TG levels and untargeted serum proteomics detects comprehensive sets of both known and novel associated proteins likely reflecting regulation of lipid metabolism and inflammation. Future proteomics study with metabolomics may lead to new biomarkers and metabolic pathways underlying etiology and also may serve to identify new therapeutic targets.