Serum Proteomes and Potential Signaling Pathways Linked to Prediabetes and T2DM in the Elderly
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Abstract
Type 2 diabetes mellitus (T2DM) is a global health problem and most common in the elderly population with various causes. The present study aimed to investigate the different expressions of serum proteins in Thai elderly population with and without diabetes. Data and serum were based on the EGAT cohort study (2012), with participants aged 60-80 years classified into two study groups; the control group (N=9) were healthy men without metabolic syndrome and the prediabetes/T2DM (N=35). Identification of serum proteome profile by liquid chromatography-tandem mass spectrometry was performed. Nineteen unique proteins found only in the prediabetes and T2DM involved in glycosylation and transport of proteins and lipids, growth factor activity, and inflammatory responses. Analysis of protein-protein interaction among proteins with relative fold change >1.5 in the prediabetes and T2DM compared to the control group showed association with PI3K-AKT signaling path for up-regulated proteins and MAPK pathway way for down-regulated proteins. Both pathways play role in pancreatic beta cell function and regulation of insulin response. In conclusion, overall findings can lead to elucidate pathways of T2DM and contribute to important information for advance therapeutic strategies for T2DM and its complications in elderly population.
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