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Genetic characterization of goat breeds and populations in Egypt is essential for conservation strategy as well as for genetic improvement. The aim of this study was to determine the genetic diversity of goat breeds and population in Egypt using microsatellite markers for proper utilization in breeding program. Six microsatellite markers (TCRVB6, BM6444, ETH10, TGLA53, McM527 and CSRD247) were used for the analysis of 56 goat individuals from four local goat genotypes. The number of alleles per locus ranged from 3 (loci TGLA53 and CSRD247) to 8 (locus ETH10). Allele size of the six loci (TCRVB6, BM6444, ETH10, TGLA53, McM527 and CSRD247) ranged from 210-294, 130-262, 148-239, 128-160, 152-203 and 210-244 bp in Baladi, Zaraibi, Demusces and Farafra goats breeds, respectively. The average values of observed heterozygosity were 0.155, 0.211, 0.100 and 0.222 in Baladi, Zaraibi, Demusces and Farafra goats, respectively. The average values of expected heterozygosity were 0.619, 0.658, 0.431 and 0.632 in Baladi, Zaraibi, Demusces and Farafra goats, respectively. Population fixation indices were Fst = 0.2148 referring to the highest variation among individuals within populations while, Fit = 0.754 referring to differences the lowest in variation of within individuals. A pair wise difference among Baladi, Zaraibi, Demusces and Farafra goats was recorded (0.6863) among populations F index (Fis). Moreover, five specific alleles were observed in Baladi goat breed and Farafra goat population. The suggesting alleles as genotype fingerprint (even it was one allele for one locus) could be used for the differentiation between the goats.
Keywords: genetic diversity, Egyptian goat breeds, populations, microsatellite markers
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 Galal, S., 2007. Farm animal genetic resources in Egypt; Factsheet. Egypt. J. Anim. Prod., 44 (1), 1-23.
 Hassanane, M.S., El-Kholy, A.F., El-Rahman, A.R.A. and Somida, M. R., 2010. Genetic variations of two Egyptian goat breeds using microsatellite markers. Egypt. J. Anim. Prod., 47(2), 93-105.
 Elbeltagy, A.R., 2012. Characterization and value addition to local breeds and their products in the Near East and North Africa. FAO report, Regional Workshop, Rabat, Morocco.
 Marai, I.F.M., Ayyat, M.S. and El-Monem U.M.A., 2001. Growth performance and reproductive traits at first parity of New Zealand white female rabbits as affected by heat stress and its alleviation under Egyptian conditions, Trop. Anim. Health Product., 33, 457-462.
 Jnied, A.M., Rashed, M.A. and Anous, M.R., 2012. Molecular genetic characterization for two Egyptian goat breeds. Egypt. J. Genet. Cytol., 41, 285- 296.
 Agha, S.H., Pilla, F., Galal, S., Shaat, I., Andrea, M.D., Reale, S., Abdelsalam, A.Z.A. and Li, M.H., 2008. Genetic diversity in Egyptian and Italian goat breeds measured with microsatellite polymorphism. J. Anim. Breed. Genet., 125, 194-200.
 Takezaki, N. and Nei M., 1996. Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genet., 144(1) 389-399.
 Diez-Tascón, C., Littlejohn, R.P., Almeida, P.A.R. and Almeida, M.C.A., 2000. Genetic variation within the Merino sheep breed: analysis of closely related populations using microsatellites. Anim. Genet., 31, 243-251.
 Moioli B., Georgoudis, A., Napolitano, F., Catillo, G., Giubilei, E., Ligda, C.H. and Hassanane, M., 2001. Genetic diversity between Italian, Greek and Egyptian buffalo populations. Livest. Prod. Sci., 70, 203-211.
 Li, M.H., Zhao, S.H., Bian, C., Wang, H.S., Wei, H., Liu, B., Yu, M., Fan, B., Chen, S.L., Zhu, M.J., Li, S.J., Xiong, T.A. and Li, K., 2002. Genetic relationships among twelve Chinese indigenous goat populations based on microsatellite analysis. Genet. Sel. Evol., 34, 729-744.
 Visser, C., Hefer, C.A., van Marle-Koster, E. and Kotze, A., 2004. Genetic variation of three commercial and three indigenous goat populations in South Africa. South Afric. J. Anim. Sci., 34 (Supplement 1), 24-27.
 Kumar, D., Dixit, S.P., Shamrma, R., Pandey, K.A., Sirohi, G., Patel, A.K., Aggarwal, R.A.K., Verma, N.K., Gour, D.S. and Ahlawat, S.P.S., 2005. Population structure, genetic variation and management of Marwari goats. Small Rumin. Res., 5, 41-48.
 Mahrous, K.F., Saleha, Y., Alakilli, M., Lamiaa, M., Salem, S.H., El-Aziem, A. and El-Hanafy, A., 2013. Genetic diversity in Egyptian and Saudi goat breeds using microsatellite markers. J. Appl. Biosci., 72, 5838-5845.
 Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular Cloning – A Laboratory Manual, Cold Spring Harbor Laboratory Press.
 FAO. 2004. Secondary guidelines for development of national farm animal genetic resources management plans. Measurement of domestic animal diversity (MoDAD): Recommended microsatellite markers, Rome., Italy.
 FAO. 2011. Commission on Genetic Resources for Food and Agriculture thirteenth Regular Session, Draft guidelines on molecular genetic characterization of animal genetic resources, Rome.
 Yeh, F.C., Boyle, T., Rongcai, Y., Ye, Z. and Xian, J.M., 1999. POPGENE, Version 1.31. A Microsoft Window based freeware for population genetic analysis. University of Alberta, Edmonton.
 Kimura, M. and Crow, J.F., 1964. The number of alleles that can be maintained in a finite population. Genet., 68, 47.
 El-Sayed, M.A., Al-Soudy, A. and Badawy, A.A.E., 2016. Microsatellite markers Polymorphism between two Egyptian goat populations (Capra hircus). Egypt. J. Genet. Cytol., 45, 89-103.
 Wei., C., Lu, J., Xu, L., Liu, G., Wang, Z., Zhao, F., Zhang, L. Han, X. Du, L. and Liu, C. 2014. Genetic structure of Chinese indigenous goats and the special geographical structure in the Southwest China as a geographic barrier driving the fragmentation of a large population. PLos ONE 9(4): e94435. doi:10.1371/journal.pone.0094435.
 Takahashi, H., Nyamsamba, D., Mandakh, B., Zagdsuren, Y., Amano, T. and Nomura, K., 2008. Genetic structure of Mongolian goat populations using microsatellite loci analysis. Asian-Aust. J. Anim. Sci., 21(7), 947-953.
 Riyadh, S.A., Musthafa, M.M., Al-Shaikh, M.A., Badri, O.M. and Hussein, M.F., 2012. Genetic diversity of Ardi goat based on microsatellite analysis. Afric. J. Biotech., 11(100), 16539-16545.
 Visser C., van Marle-Köster, E. and Friedrich, H., 2011. Parentage verification of South African Angora goats, using microsatellite markers. South Afric. J. Anim. Sci., 41(3), 250-255.
 Marini, A., Mohd, A.B., Hifzan, R., Johari, J.A., Tan, S.G. and Panandam, J.M., 2013. Genetic variation of four goat breeds in Malaysia using microsatellite polymorphism markers. Mal. J. Anim. Sci., 16(2), 1-8.
 Thiruvenkadan, A.K., Jayakumar, V., Kathiravan, P. and Saravanan, R., 2014. Genetic architecture and bottleneck analyses of Salem Black goat breed based on microsatellite markers. Veterinary World, EISSN, 2231-0916.
 Marini, A., Hifzan, A.B., Tan, R.M. and Panandam, J.M., 2014. Assessment of genetic diversity on goat breeds in Malaysia using microsatellite markers. Mal. J. Anim. Sci., 17(1), 19-26.
 Araújo, A.M., 2006. Genetic diversity between herds of Alpine and Saanen dairy goats and the naturalized Brazilian Moxotó breed. Genet. Mol. Biol., 29(1), 67-74.
 Botstein, D., White, R.L., Skolnick, M. and Davis, R.W., 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J. Hum Genet., 32, 314-331.
 Hoda, A., 2009. Genetic diversity of the capore goat in Albania based on 30 microsatellite markers. Macedon. J. Anim. Sci., 1(1), 53-56.
 Nei, M., 1972. Genetic distance between population. Amer. Naturalist, 106(949), 283-292.