Antibacterial and Anticancer Activity of Stromatic Xylaria spp. from Tropical Forest Thailand

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Published: Jan 5, 2018
Keywords:
Xylaria, stromata, bioactive metabolites, antimicrobial, anticancer

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Krittapong Orachaipunlap
Program of Biotechnology, Faculty of science, Chulalongkorn University, Bangkok, Thailand
Nuttika Suwannasai
Departments of Biology, Srinakharinwirot University, Bangkok, Thailand
Antony J. Whalley
School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom
Sophon Roengsumran
Departments of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
Prakitsin Sihanonth*
Departments of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok,Thailand

Abstract

This research is part of a bioactive metabolite study of fungi collected from Trat Agroforestry Training Station, Trat province, Thailand. The ultimate goal is to screen for bioactivity, including those exhibiting antibacterial and anticancer, activity from isolates of stromatic Xylaria spp. Fresh specimens of Xylaria spp. were cultured on Potato Dextrose Agar (PDA) for 14 days, and then transferred to Malt Extract Broth (MEB). All isolations were cultured under static conditions for 6 weeks and extracted with ethyl acetate and concentrated by evaporation. All crude extracts were tested for antimicrobial activities against 4 pathogenic microorganisms, including Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), in terms of MIC values. Anticancer activity against 5 human cell lines was measured by MTT colorimetric assay. The results showed that all crude extracts inhibited at least 1 tested microorganism. Ten of the extracts showed a broad spectrum of action against the test bacteria. Selected Xylaria isolates were chosen for examination of their anticancer activity. Isolate TR 25 showed the highest cytotoxicity; this was against KATO-III cell line with a IC50 value of 7.8 µg.mL-1.


 Keywords: Xylaria, stromata, bioactive metabolites, antimicrobial, anticancer


*Corresponding author: Tel.: + 662-2185070


   E-mail: sprakits@chula.ac.th, okriattapong@gmail.com

Article Details

Issue
Vol. 15 No. 1 (2015)
Section
Original Research Articles

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