Inhibitions of Key Enzymes Relevant to Obesity and Diabetes of Thai Local Mushroom Extracts

Main Article Content

Tanyaporn Pongkunakorn
Thareerat Watcharachaisoponsiri
Chaowanee Chupeerach
Nattira On-nom
Uthaiwan Suttisansanee*

Abstract

The aim of this experiment was to determine inhibitory activities against lipase (anti-obesity property) as well as a-amylase and a-glucosidase (anti-diabetic property) of fifteen Thai local mushroom extracts including Kai-khao (Russula sp.), Kai-kiew (Russula sp.), Nha-lae (Russula sp.), Sa-med (Boletus sp.), E-tun (Russula aff. sp.), Ra-ngok-khao (Amanita aff. sp.), Kor-kar-dang (Russula sp.), Por-nung (Astraeus sp.), Por-fai (Astraeus sp.), Ra-ngok-luang (Amanita sp.), Kor-kar-khao (Russula sp.), Nam-mak (Macowanites sp.), Koh-noi (Russula aff. sp.), Din-khao (Russula sp.) and Khao-pang (Russula sp.) in Amnat-Charoen province, Thailand. All mushrooms were extracted using ddH2O (20 mg/mL) in a 50°C water bath shaker for 8 hrs. The enzyme inhibitory activities were performed using a colorimetric microplate assay. The results showed that anti-lipase activities of mushroom extracts (4 mg/mL) were in the range of 12-77% inhibition. Din-khao mushroom exhibited the highest inhibition, while Por-fai and Por-nung mushrooms exhibited the lowest. The anti-a-glucosidase activities of mushroom extracts (5 mg/mL) were in the range of 10-79% inhibition. Sa-med mushroom exhibited the highest inhibition, while Kor-kar-dang mushroom exhibited the lowest. However, the inhibition of a-amylase could not be detected in this study. These findings are useful for potential health promotion for Thai local mushroom consumption in controlling obesity and diabetes through key enzymes inhibition.


Keywords: enzyme inhibitory activity, obesity, diabetes mellitus, Thai local mushroom


*Corresponding author: Tel.: 0-2800-2380 ext. 422; Fax: 0-2441-9344


 E-mail: uthaiwan.sut@mahidol.ac.th

Downloads

Download data is not yet available.

Article Details

Section
Research Articles

References

[1] Mizutani, T., Inatomi, S., Inazu, A. and Kawahara, E., 2010. Hypolipidemic effect of Pleurotus eryngii extract in fat-loaded mice. Journal of Nutritional Science and Vitaminology, 56, 48-53.
[2] Mihailovic, M., Jovanovic, J.A., Uskokovic, A., Grdovic, N., Dinic, S., Vidovic, S., Poznanovic, G., Mujic, J. and Vidakovic, M., 2015. Protective effects of the mushroom Lactarius deterrimus extract on systemic oxidative stress and pancreatic islets in streptozotocin-induced diabetic rats. Journal of Diabetes Research, 2015, 1-10.
[3] Ozcelik, O., Dogan, H. and Kelestimur, H., 2004. Effects of a weight-reduction program with orlistat on serum leptin levels in obese women: A 12 week, randomized, placebo-controlled study. Current Therapeutic Research, 65(2), 127-137.
[4] Funke, I. and Melzig, F.M., 2006. Traditionally used plants in diabetes therapy - phytotherapeutics as inhibitors of α-amylase activity. Sociedade Brasileira de Farmacognosia, 16(1), 1-5.
[5] Watcharachaisoponsiri, T., 2016. Anti-obesity and anti-diabetic properties of Thai local chili pepper: enzyme inhibitory studies and bioactive compounds identification. Mahidol University.
[6] Cheung, P.C.K., 2013. Mini-review on edible mushrooms as source of dietary fiber: Preparation and health benefits. Food Science and Human Wellness, 2(3-4), 162-166.
[7] Liu, J., Jia, L., Kan, J. and Jin, C.H., 2013. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food and Chemical Toxicology, 51, 310-316.
[8] Stachowiak, B. and Reguła, J., 2012. Health-promoting potential of edible macromycetes under special consideration of polysaccharides: a review. European Food Research and Technology, 234(3), 369-380.
[9] Lee, J.K., Jang, J.H., Lee, J.T. and Lee, J.S., 2010. Extraction and characteristics of anti-obesity lipase inhibitor from Phellinus linteus. Mycobiology, 38(1), 52-57.
[10] Palanisamy, M., Gil-Ramírez, A., Ruiz-Rodríguez, A., Marín, F.R., Reglero, G. and Soler-Rivas, C., 2012. Testing edible mushrooms to inhibit the pancreatic lipase activity by an in vitro digestion model. International Journal of Food Science and Technology, 47(5), 1004-1010.
[11] Su, C.H., Lai, M.N. and Ng, L.T., 2013. Inhibitory effects of medicinal mushrooms on alpha-amylase and alpha-glucosidase-enzymes related to hyperglycemia. Food and Function, 4(4), 644-649.
[12] Pongkunakorn, T. and Suttisansanee, U., 2016. Total phenolic contents and antioxidant activities of Thai local mushrooms. Agricultural Science Journal, 47(2), 401-404.
[13] Choi, S.J., Hwang, J.M. and Kim, S.I., 2003. A colorimetric microplate assay method for high throughput analysis of lipase activity. Journal of Biochemistry and Molecular Biology, 36(4), 417-420.
[14] You, Q., Chen, F., Wang, X., Luo, P.G. and Jiang, Y., 2011. Inhibitory effects of muscadine anthocyanins on alpha-glucosidase and pancreatic lipase activities. Journal of Agricultural and Food Chemistry, 59(17), 9506-9511.
[15] Slanc, P., Doljak, B., Mlinari, A. and Itrukel, B., 2004. Screening of wood damaging fungi and macrofungi for inhibitors of pancreatic lipase. Phytotherapy Research, 18, 758-762.
[16] Cai, S., Wang, O., Wang, M., He. J., Wang, Y., Zhang, D., 2012. In vitro inhibitory effect on pancreatic lipase activity of subfractions from ethanol extracts of fermented oats (Avena sativa L.) and synergistic effect of three phenolic acids. Journal of Agricultural and Food Chemistry, 60(29), 7245-7251.


[17] Gondoin, A., Grussu, D., Stewart, D. and McDougall, G.J., 2010. White and green tea polyphenols inhibit pancreatic lipase in vitro. Food Research International, 43(5), 1537-1544.
[18] McDougall, G.J., Kulkarni, N.N. and Stewart, D., 2009. Berry polyphenols inhibit pancreatic lipase activity in vitro. Food Chemistry, 115(1), 193-199.
[19] Gil-Ramírez, A., Pavo-Caballero, C., Baeza, E., Baenas, N., Garcia-Viguera, C., Marín, F.R., 2016. Mushrooms do not contain flavonoids. Journal of Functional Foods, 25, 1-13.
[20] Friedman, M., 2016. Mushroom polysaccharides: Chemistry and antiobesity, antidiabetes, anticancer, and antibiotic properties in cells, rodents, and humans. Foods, 5(4), 1-40.
[21] Lee, J.K., Song, J.H. and Lee, J.S., 2010. Optimal extraction conditions of anti-obesity lipase inhibitor from Phellinus linteus and nutritional characteristics of the extracts. Mycobiology, 38(1), 58-61.
[22] Rathee, S., Rathee, D., Rathee, D., Kumar, V. and Rathee, P., 2012. Mushrooms as therapeutic agents. Revista Brasileria de Farmacognosia Brazilian Journal of Pharmacognosy, 22(2), 459-474.
[23] Wichaidit, T., 2011. In vitro inhibitory effect of edible mushroom extracts on alpha amylase and alpha glucosidase enzymes, Rangsit University.
[24] Matsuur, H., Asakawa, C., Kurimoto, M. and Mizutani, J., 2002. Alpha-glucosidase inhibitor from the seeds of balsam pear (Momordica charantia) and the fruit bodies of Grifola frondosa. Bioscience, Biotechnology and Biochemistry, 66(7), 1576-1578.
[25] Gusakov, A.V., Kondratyeva, E.G. and Sinitsyn, A.P., 2011. Comparison of two methods for assaying reducing sugars in the determination of carbohydrate activities. International Journal of Analytical Chemistry, 2014, 1-4.
[26] Yuan, Z., He, P., Cui, J. and Takeuchi, H., 1998. Hypoglycemic effect of water-soluble polysaccharide from Auricularia auricula-judae Quel. on genetically diabetic KK-Ay mice. Bioscience, Biotechnology and Biochemistry, 62(10), 1898-1903.
[27] Zhang, H.N. and Lin, Z.B., 2004. Hypoglycemic effect of Ganoderma lucidum polysaccharides. Acta Pharmacologica Sinica, 25, 191-195.
[28] Kim, Y.W., Kim, K.H., Choi, H.J. and Lee, D.S., 2005. Anti-diabetic activity of beta-glucans and their enzymatically hydrolyzed oligosaccharides from Agaricus blazei. Biotechnology Letters, 27(7), 483-487.
[29] Yang, B.K., Jung, Y.S. and Song, C.H., 2007. Hypoglycemic effects of Ganoderma applanatum and Collybia confluens exo-polymers in streptozotocin-induced diabetic rats. Phytotherapy Research, 21(11), 1066-1069.
[30] Muraoka, O., Morikawa, T., Miyake, S., Akaki, J., Ninomiya, K. and Yoshikawa, M., 2010. Quantitative determination of potent alpha-glucosidase inhibitors, salacinol and kotalanol, in Salacia species using liquid chromatography-mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 52(5), 770-773.
[31] Yin, Z., Zhang, W., Feng, F., Zhang, Y. and Kang W., 2014. α-Glucosidase inhibitors isolated from medicinal plants. Food Science and Human Wellness, 3(3–4), 136-74.