Ethanol Production on Jackfruit Seeds by Selected Fungi and Yeast from Loog-pang
Article Sidebar
Main Article Content
Abstract
Ethanol production from jackfruit seeds was carried out by using fungal strains isolated from tankoji(loog-pang). These were Rhizopus oryzae MNT 006, Aspergillus oryzae MNT 029, Amylomyces rouxii MNT 037 and Saccharomyces cerevisiae YRK 017. Fermentation sugar production from jackfruit seeds starch by 3 fungal strains was examined. Then the fermentable sugars produced so were tested as substrates for fermentation by S. cerevisiae YRK 017 to produce ethanol. The results showed that A. rouxii was the most effective organism for producing reducing sugar. Ethanol production process was studied on separated hydrolysis and fermentation (SHF) process and simultaneous saccharification and fermentation (SSF) process. The jackfruit seeds starch fermented by SHF process produced higher yield of ethanol than that from SSF process.
Keywords: ethanol production, jackfruit seed, separated hydrolysis and fermentation, simultaneous saccharification and fermentation
E-mail: kodaungj@kmitl.ac.th
Article Details
Copyright Agreement Statement
The corresponding author has to submit Copyright Agreement form after the article is accepted for publication in order to warrant that this contribution is original and that he/she has full power to make this grant. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors.
The author(s) grant Current Applied Science and Technology a non-exclusive, irrevocable, royalty-free license to publish, reproduce, distribute, and archive the article in print and electronic form with effect if and when the article is accepted for publication. In the event that the article is withdrawn prior to acceptance or is declined, this agreement shall have no effect, and no party shall be bound by it.
The author(s) retain copyright of this article, including but not limited to the right to reproduce and distribute the article, to include it in a thesis or book, and to post it on an institutional or personal repository, provided that the original publication in Current Applied Science and Technology is properly cited.
References
[2] Montesinos, T. and Navarro, J. M., 2000. Production of alcohol from raw wheat flour by amyloglucosidase and Saccharomyces cevevisiae. Enzyme and Microbial Technology, 27, 362-372.
[3] John, R. P., Anisha, G. S., Nampoothiri, K. M. and Pandey, A., 2009. Direct lactic acid fermentation focus on simultaneous saccharification and lactic acid production. Biotechnology Advance, 27, 145-152
[4] Soderstrom, J., Galbe, M. and Zacchi, G., 2005. Separate versus simultaneous saccharification and fermentation of two-step steam pretreated softwood for ethanol production. Journal of Wood Chemistry and Technology, 25,187-202
[5] Bandaru, V. V. R., Somalanka, S. R., Mendu, D. R., Madicherla, N. R. and Chityala, A., 2006. Optimization of fermentation conditions for the production of ethanol from sago starch by co-immobilized amyloglucosidase and cells of Zymomonas mobilis using response surface methodology. Enzyme and Microbial Technology, 38, 209-214.
[6] Jamai, L., Ettayebi, K., Yamani, J. E. L. and Ettayebi, M., 2007. Production of ethanol from starch by free and immobilized Candida tropicalis in the presence of amylase. Bioresource Technology, 98, 2765-2770.
[7] Ebrahimi, F., Khanahmadi, M., Roodpeyma, S. and Taherzadeh, M. J., 2008. Ethanol production from bread residues. Biomass Bioenergy, 32, 333-337.
[8] Bobbio, F. O., El-Dash, A. A., Bobbio, P. A. and Rodrigues, L. R., 1978. Isolation and characterization of the physicochemical properties of the starch of jackfruit seeds (Artocarpus heterorphyllus). Cereal Chemistry, 55, 505-511.
[9] Kumar, S., Singh, A. B., Abidi, A. B., Upadhyay, R. G. and Singh, A., 1988. Proximate composition of jack fruit seeds. Journal of Food Science and Technology. 25, 308-309.
[10] Rattanapreedakul, W., 2006. Study on fungi and yeast isolated from Look-Pang in comparison with pure culture for sato production. Master Degree. King Mongkut’s Institute of Technology Ladkrabang.
[11] Nelson, N. A., 1944. Photometric adaptation of somogyi method for the determination of glucose. Journal of Biological Chemistry. 15, 375-380.
[12] Marques, S., Alves, L., Roseiro, J. C., Girio, F.M., 2008. Conversion of recycled paper sludge to ethanol by SHF and SSF using Pichia stipitis. Biomass and Bioenergy, 32, 400-406.
[13] Ochaikul, D., Poungmalee, B., Yimyai, T. and Keawkaew, S., 2010. Production of ethanol from jackfruit seeds starch by enzymatic hydrolyzation and Saccharomyces cerevisiae fermentation. In: Proceeedings NZMS and NZSBMB Joint Meeting, 30 November -3 December, The University of Auckland, New Zealand, 65-66.
[14] Ooshima, H., Ishitani,Y. and Harano, Y., 1985. Simultaneous saccharification and fermentation of cellulose-effect of ethanol on enzymatic saccharification of cellulose. Biotechnology and Bioengineering, 27,389-397.
