Optimization of Air Temperature and Medium pH Enhanced Growths and 1-Acetoxychavicol Acetate (ACA) Content of Galangal (Alpinia galanga) Plantlets in vitro
Article Sidebar
Main Article Content
Abstract
Galangal (Alpinia galanga) is a tropical rhizome-producing plant that produced 1'-acetoxychavicol acetate (ACA) which has been used as a traditional herb in South-Eastern Asian. However, the environmental factors in the fields such as microorganisms, heavy metal, dust, etc. affected the growth and ACA content in galangal. Therefore, the aim of this research was to investigate the responses of growths and ACA content of galangal plantlets on different air temperatures and medium pH. The galangal in vitro plantlets were cultured on modified Murashige and Skoog (1962) under different air temperatures (15, 20, 25, 30 and 35°C) and medium pH (4.0, 5.5, 7.0 and 8.5) for 5 weeks. Fresh weight and dry weight of shoots, roots and rhizomes, and ACA content were assessed. The fresh weight and dry weight of shoots, roots and rhizomes were enhanced at 20-35°C air temperature. The ACA content in galangal rhizomes was greatest at 30°C air temperature (28.0 g g-1 rhizome dry weight). In addition, the medium pH at 5.5-7.0 promoted the fresh weight and dry weight of shoots, roots and rhizomes of galangal plantlets. The ACA content in galangal rhizomes was greatest when cultured on medium pH at 7.0 (28.2
g g-1 rhizome dry weight). Moreover, the air temperature and medium pH were positively related to ACA production (r2= 0.97 and r2= 0.90, respectively). It is deliberated that the growth and ACA content in galangal in vitro plantlets can be promoted under condition of 30°C air temperature and medium pH at 7.0.
Keywords: Zingiberaceae, secondary metabolite, medicinal plant, plant tissue culture
E-mail: [email protected]
Article Details
Copyright Transfer Statement
The copyright of this article is transferred to Current Applied Science and Technology journal with effect if and when the article is accepted for publication. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this contribution is original and that he/she has full power to make this grant. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors.
Here is the link for download: Copyright transfer form.pdf
References
[2] Murakami, A., Jiwajinda, S., Koshimizu, K. and Ohigashi, H., 1995. Screening for invitro anti-tumor promoting activities of edible plants from Thailand. Cancer Letters, 95(1), 139-146.
[3] Palittapongarnpim, P., Kirdmanee, C., Kittakoop, P. and Rukseree, K., 2002. 1'-Acetoxychavicol Acetate for Tuberculosis Treatment. USA. Pat. 2002192262.
[4] Matsuda, H., Morikawa, T., Managi, H. and Yoshikawa, M., 2003. Antiallergicprinciplesfrom Alpinia galanga: structural requirements of phenylpropanoids for inhibition of degranulation and release of TNF-α and IL-4 in RBL-2H3 cells. Bioorganic & Medicinal Chemistry Letters, 13(19), 3197-3202.
[5] Ye, Y. and Li, B., 2006. 1S-1-Acetoxychavicol acetate isolated from Alpinia galangal inhibits human immunodeficiency virus type 1 replication by blocking Rev transport. Journal of General Virology, 87(7), 2047-2053.
[6] Lauglin, J.C. and Munro, D., 1982. The effect of fungal colonization on the morphine production of poppy Papaver somniferum L. capsules. The Journal of Agricultural Science,98(3), 679-687.
[7] Bernath, J., 1986. Production ecology of secondary plant products. In L.E. Cracker and J.E.Simon, eds. 1986. Herbs, Spices and Medicinal Plants. Recent Advance in Botany,Horticulture, and Pharmacology, Vol 1. Oryx Press, pp. 185-234.
[8] Siringam, E., 2004. Factors 1'- Acetoxychavicol Acetate (ACA) production in galangal (Alpinia galanga (L.) willd.). Master of Science (Agriculture). Kasetsart University.
[9] Couceiro, M.A., Afreen, F., Zobayed, S.M.A. and Kozai, T., 2006. Variation in concentrations of major bioactive compounds of St. John’s wort: Effects of harvesting time, temperature and germplasm. Plant Science, 170(1), 128-134.
[10] Murch, S.J., Choffe, K.L., Victor, J.M.R., Slimmon, T.Y., Raj, S.K. and Saxena, P.K., 2000. Thidiazuron-induced plant regeneration from hypocotylscultures of St. John’s wort plants(Hypericum perforatum cv. ‘Anthos’). Plant Cell Reports, 19(6), 576-581.
[11] Saxena, P.K. and Zobayed, S.M.A., 2004. Production of St. John’s wort plants under controlled environment for maximizing biomass and secondary metabolites. In Vitro Cellular & Development Biology - Plant, 40(1), 108-114.
[12] Arditti, J. and Ernst, R., 1993. Micropropagation of Orhids. John Wiley & Sons, Inc.
[13] Jayapaul, K., Kavikishor, P.B. and Reddy, K.J., 2005. Production of pyrroloquinazoline alkaloid from leaf and petiole-derived callus cultures of Adhatoda zeylanica. In Vitro Cellular & Development Biology - Plant, 41(5), 682-685.
[14] de Abreu, I.N., Sawaya, A.C.H.F., Eberlin, M.N. and Mazzafera, P., 2005. Production of pilocarpine in callus of jaborandi (Pilocarpus microphyllus Stapf). In Vitro Cellular & Development Biology - Plant, 41(6), 806-811.
[15] Maurmann, N., De Carvalho, C.M.B., Silva, A.I.L., Fett-Neto, A.G., Von Poser, G.L. and Rech, S.B., 2006. Valepotriates accumulation in callus, suspended cells and untransformed root cultures of Valeriana glechomifolia. In Vitro Cellular & Development Biology - Plant,42(1), 50-53.
[16] Arumugam, N. and Bhojwani, S.S., 1990. Somatic embryogenesis in tissue cultures of Podophyllum hexandrum. Canadian Journal of Botany, 68(3), 487-491.
[17] Zhong, J.J. and Yoshida, T., 1993. Effects of temperature on cell growth and anthocyanin production by suspension cultures of Perilla frutescens cells. Journal of Fermentation and Bioengineering, 76(6), 530-531.
[18] Gianoli, E. and Niemeyer, H.M., 1997. Environmental effects on the accumulation of hydroxamic acids in wheat seedlings: the importance of plant growth rate. Journal of Chemical Ecology, 23(2), 543-551.
[19] ten Hoopen, H.J.G., Vinke, J.L., Moreno, P.R.H., Verpoorte, R. and Heijnen, J.J., 2002. Influence of temperature on growth and ajmalicine production by Catharantus roseus suspension cultures. Enzyme and Microbial Technology, 30(1), 56-65.
[20] Zobayed, S.M.A., Afreen, F. and Kozai, T., 2005. Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’s wort. Plant Physiology and Biochemistry, 43(10-11), 977-984.
[21] Shohael, A.M., Chakrabarty, D., Yu, K.W., Hahn, E.J. and Paek, K.Y., 2005. Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides. Journal of Biotechnology,120(2), 228-236.
[22] Shohael, A.M., Ali, M.B., Yu, K.W., Hahn, E.J. and Paek, K.Y., 2006. Effect of temperature on secondary metabolites production and antioxidant enzyme activities in Eleutherococcus senticosus somatic embryos. Plant Cell, Tissue and Organ Culture, 85(2), 219-228.
[23] Pasqua, G., Manes, F., Monacelli, B., Natale, L. and Anselmi, S., 2002. Effects of the culture Medium pH and ion uptake in in vitro vegetable organogenesis in thin cell layers of tobacco.Plant Science, 162(6), 947-955.
[24] Taiz, L. and Zieger E., 2002. Plant Physiology. 3rd ed. Sinauer Associate, Inc.Publisher.
[25] Nilsson, T., 1970. Studies into the pigments in beet root (Beta vulgaris L. Sp. Vulgaris var.rubra L). Lantbrukshogsk Annual, 36, 179-219.
[26] Mukundan, U., Bhide, V., Singh, G. and Curtis, W.R., 1998. pH-mediated release of betalaines from transformed root cultures of Beta vulgaris L. Applied Microbiology and Biotechnology, 50(2), 241-245.
[27] Hattori, T. and Ohta, Y., 1985. Induction of phenylalanine ammonialyase activation and isoflavones glucoside accumulation in suspension-cultured cells of red bean, Vigna angularis,by phytoalexin elicitors, vanadate, and elevation of medium pH. Plant & Cell Physiology,26(6), 1101-1110.
[28] Saenz-Carbonell, L.A., Maldoredo-Mendoza, I.E., Moreno-Valenzula, O., Ciau-Uitz, R.,Lopez-Meyer, M., Oropeza, C. and Loyola-Vorgans, V.M., 1993. Effect of medium pH on the release of secondary metabolites from roots of Datura stramonium, Catheranthus roseas and Tagetes patula cultured in vitro. Applied Biochemistry and Biotechnology, 38(3), 257-267.
[29] Wu, J. and Zhong, J.J., 1999. Production of ginseng and its bioactive components in plant cell culture: Current technological and applied aspects. Journal of Biotechnology, 68(2-3), 89-99.
[30] Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-479.
[31] Lutts, S., Kinet, J.M. and Bouharmont, J., 1996. NaCl induced senescence in leaves of rice(Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78(3), 389-398.
[32] Nilsen, E.T. and Orcutt, D.M., 1996. The Physiology of Plants under Stress. John Wiley & Sons, Inc.
[33] Levitt, J., 1980. Responses of Plants to Environmental Stresses Vol. I. Academic Press.
[34] Dixon, R.A. and Paiva, N.L., 1995. Stress-induced phenylpropanoid metabolism. The Plant Cell, 7(7), 1085-1097.
[35] Zhang, Y.H. and Zhong, J.J., 1997. Hyperproduction of ginseng saponin and polysaccharide by high density cultivation of Panax notoginseng. Enzyme and Microbial Technology, 21(1),
59-63.
[36] Marschner, H., Horst, W.J., Martin, P. and Romheld, V., 1986. Root induced changes in the rhizosphere: Importance for the mineral nutrition of plants. Zeitschrift fur Pflanzenernahrung und Bodenkunde, 149(4), 441-456.
[37] Hagendoorn, M.J., Wagner, A.M., Segers, G., Van der Plas, L.H.W., Oostdam, A. and Van Walraven, H.S., 1994. Cytoplasmic acidification and secondary metabolite production in different plant cell suspensions. Plant Physiology, 106(2), 723-730.
[38] Henkes, S., Sonnewald, U., Badur, R., Flachmann, R. and Stitt, M., 2001. A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism. The Plant Cell, 13(3), 535-551.
[39] Waloszek, A. and Wieckowski, S., 2004. Effects of pH on the kinetics of light-dependent photon flux in thylakoids isolated from lettuce leaves. Plant Science, 166(2), 479-483.