Growth Inhibition of Aspergillus flavus IMI 242684 by Crude Extract of Penicillium sp.
Article Sidebar
Published:
Mar 30, 2018
Keywords:
aflatoxin, Aspergillus flavus, Penicillium, crude extrac
Main Article Content
Abstract
Crude extract of Penicillium sp. isolate 480519 I06 ( 3) was tested for an ability to inhibit the growth of Aspergillus flavus IMI 242684 using paper disc diffusion assay on Sabouraud Dextrose Agar (SDA ). The effectiveness of extracts from culture filtrates and mycelia by different solvents,hexane, ethyl acetate, butanol and methanol, using liquid-liquid extraction were compared. The results show that hexane and ethyl acetate extracts from culture filtrates were most active against A. flavus IMI 242684. Hexane extract at 100 mg/ml gave the highest inhibitory zone of ~ 17.50 mm followed by ethyl acetate extract at 100 mg/ml ( inhibitory zone of ~ 9.87 mm) and hexane extract at 50 mg/ml (inhibitory zone of ~ 9.75 mm).
Keywords: aflatoxin, Aspergillus flavus, Penicillium, crude extrac
Article Details
Issue
Section
Short Communications
Copyright Transfer Statement
The copyright of this article is transferred to Current Applied Science and Technology journal with effect if and when the article is accepted for publication. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this contribution is original and that he/she has full power to make this grant. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors.
Here is the link for download: Copyright transfer form.pdf
References
[1] Wilson, D.M. and Payne, G.A., 1994. Factors affecting Aspergillus flavus group infection and aflatoxin contamination of crops. In: D.L. Eaton and J.D. Groopman, ed. 1994. The Toxicology of Aflatoxins. Human Health, Veterinary, and Agricultural Significance, San Diego, Academic Press, Inc., pp. 383-406.
[2] Goto, T., Wicklow, D.T. and Ito, Y., 1996. Aflatoxin and cyclopiazonic acid production by a sclerotium - producing Aspergillus tamarii strain. Applied and Environmental Microbiology,62, 4096-4038.
[3] Carlile, M.J., Watkinson, S.C. and Gooday, G.W., 2001. The Fungi. 2nd ed. San Diego,Academic Press.
[4] Ito, Y., Peterson, W., Wicklow, D.T. and Goto, T., 2001. Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi, Mycological Research, 105(2), 233-239.
[5] Peterson, S.W., Ito, Y., Horn, B.W. and Goto, T., 2001. Aspergillus bombycis, a new aflatoxigenic species and genetic variation in its sibling species, A. nomius, Mycologia, 93,689-703.
[6] Godet, M. and Manaut, F., 2010. Molecular strategy for identification in Aspergillus section Flavi, FEMS Microbiological Letter, 304(2), 157-168.
[7] Bommakanti, A.S. and Waliyar, F., 2007. Importance of aflatoxins in human and livestock health. [online]. Available at: http://www.aflatoxin.info/health.asp
[8] Smith, J.E. and Moss, M.O., 1985. Mycotoxins. Formation, Analysis and Significance. Chichester, John Wiley & Sons.
[9] Montesano, R., Hainaut, P. and Wild, C.P., 1997. Hepatocellular carcinoma: From gene to public health. Review, Journal of National Cancer Institute, 89, 1844-51.
[10] Groopmann, J.D. and Kensler, T.W., 1988. Aflatoxin exposure in human populations:measurements and relationship to cancer. CRC Critical Reviews in Toxicology, 19, 113-124.
[11] Tassaneeyakul, W., Razzazi-Fazeli, E., Porasuphatana, S. and Bohm, J., 2004. Contamination of aflatoxins in herbal medicinal products in Thailand, Mycopathologia, 158,239-244.
[12] Promhirangul, P., 1999. Study of Aflatoxin Content in Noodle Dishes sold in Three Areas of Bangkok. M.Sc. Thesis, Faculty of Graduate Studies, Mahidol University, Bangkok,Thailand.
[13] Thanaboripat, D., 2002. Importance of aflatoxins, KMITL Science Journal, 2(1), 38-45.
[14] Mondali, N.K., Mojumdar, A., Chatterje, S.K., Banerjee, A., Datta, J.K. and Gupta, S., 2009. Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium, Journal of Applied Science and Environmental Management, 13(1), 49-53.
[15] Campbell, R., 1989. Biological Control of Microbial Plant Pathogens. New York,Cambridge University Press.
[16] Calistru, C., Mclean, M. and Berjak, P., 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichodema species. I.Macroscopial and microscopical observations of fungal interactions, Mycopathologia, 139,115-121.
[17] Santamarina, M.P., Roselló, J., Llacer, R. and Sanchis, V., 2002. Antagonistic activity of Penicillium oxalicum Corrie and Thom, Penicillium decumbens Thom and Trichoderma harzianum Rifai isolates against fungi, bacteria and insects in vitro, ReviewIberoam Micology, 19, 99-103.
[18] Thanaboripat, D., Sappakitjanon, N., Prommi, L. and Chareonsettasilp, S., 2009. Screening of fungi for the control of Aspergillus parasiticus, KMITL Science and Technology Journal,9(2), 95-102.
[19] Wongsariya, K., Kuntonwan, S. and Sangvorawanna, S., 2005. Screening of Trichoderma spp. for Inhibition of Aflatoxin Producing Fungi. A Special Project. Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
[20] Nguefack, J., Leth, V., Zollo, A.P.H. and Mathur, S.B., 2004. Evaluation of five essential oils from aromatic plant of Cameroon for controlling food spoilage and mycotoxin producing fungi, International Journal of Food Microbiology, 94, 329-334.
[21] Calistru, C., Mclean, M. and Berjak, P., 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species: A study of the production of extracellular metabolites by Trichoderma species, Mycopathologia, 137,115-124.
[22] Vizcaino, J.A., Sanz, L., Basilio, A., Vicente, F., Gutierrez, S., Hermosa, M.R. and Monte,E., 2005. Screening of antimicrobial activities in Trichoderma isolate representing three Trichoderma sections, Mycological Research, 109(12), 1397-1406.
[23] Se-Gul, J., Kyung-Su, J., Eun-Hee, L., Won-Sik, K. and Jae-Yong, C., 2009. Isolation of 1’,3’- Dilinolenoyl-2’-Linoleoylglycerol with tyrosinase inhibitory activity from Flammulina velutipes, Journal of Microbiology and Biotechnology, 19(7), 681-684.
[24] Lorian, V., 1986. Antibiotics in Laboratory Medicine. Philadelphia, Lippincott Williams&Wilins.
[25] Jantarach, J. and Thanaboripat, D., 2010. The efficacy of ethyl acetate extract of Trichoderma culture broth on growth inhibition and aflatoxin production by Aspergillus flavus IMI 242684, KMITL Science and Technology Journal 10(1), 19-29.
[26] Yin, G., Wang, W., Sha, S., Liu, L. and Yu, X., 2010. Inhibition and control effects of the ethyl acetate extract of Trichoderma harzianum fermented broth against Botrytis cinerea,African Journal of Microbiology Research, 4(15), 1647-1653.
[27] Febles, C.I., Arias, A., Gil-Rodriguez, M.C., Hardisson, A. and Sierra Lopez, A., 1995. In vitro study of antimicrobial activity in algae (Chrorophyta, Phaeophyta and Rodophyta) collected from the coast of Tenerife, Anuario del Instituto de Estudios Canarios, 34, 181-192.
[28] Luckner, M., 1990. Secondary Metabolism in Microorganisms, Plants and Animals. 3rd ed. Springer-Verlag, Berlin.
[29] Yang, L., Xie, J., Jiang, D., Fu, Y., Li, G. and Lin, F., 2008. Antifungal substances produced by Penicillium oxalicum strain PY-1-potential antibiotics against plant pathogenic fungi. World Journal of Microbiological Biotechnology, 24, 909-915.
[30] Ma, Y., Chang, Z-Z., Zhao, J-T., and Zhou, M-G., 2008. Antifungal activity of Penicillium stratisporum Pst10 and its biocontrol effect on Phytophthora root rot of chilli pepper,Biological Control, 44(1), 24-31.
[2] Goto, T., Wicklow, D.T. and Ito, Y., 1996. Aflatoxin and cyclopiazonic acid production by a sclerotium - producing Aspergillus tamarii strain. Applied and Environmental Microbiology,62, 4096-4038.
[3] Carlile, M.J., Watkinson, S.C. and Gooday, G.W., 2001. The Fungi. 2nd ed. San Diego,Academic Press.
[4] Ito, Y., Peterson, W., Wicklow, D.T. and Goto, T., 2001. Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi, Mycological Research, 105(2), 233-239.
[5] Peterson, S.W., Ito, Y., Horn, B.W. and Goto, T., 2001. Aspergillus bombycis, a new aflatoxigenic species and genetic variation in its sibling species, A. nomius, Mycologia, 93,689-703.
[6] Godet, M. and Manaut, F., 2010. Molecular strategy for identification in Aspergillus section Flavi, FEMS Microbiological Letter, 304(2), 157-168.
[7] Bommakanti, A.S. and Waliyar, F., 2007. Importance of aflatoxins in human and livestock health. [online]. Available at: http://www.aflatoxin.info/health.asp
[8] Smith, J.E. and Moss, M.O., 1985. Mycotoxins. Formation, Analysis and Significance. Chichester, John Wiley & Sons.
[9] Montesano, R., Hainaut, P. and Wild, C.P., 1997. Hepatocellular carcinoma: From gene to public health. Review, Journal of National Cancer Institute, 89, 1844-51.
[10] Groopmann, J.D. and Kensler, T.W., 1988. Aflatoxin exposure in human populations:measurements and relationship to cancer. CRC Critical Reviews in Toxicology, 19, 113-124.
[11] Tassaneeyakul, W., Razzazi-Fazeli, E., Porasuphatana, S. and Bohm, J., 2004. Contamination of aflatoxins in herbal medicinal products in Thailand, Mycopathologia, 158,239-244.
[12] Promhirangul, P., 1999. Study of Aflatoxin Content in Noodle Dishes sold in Three Areas of Bangkok. M.Sc. Thesis, Faculty of Graduate Studies, Mahidol University, Bangkok,Thailand.
[13] Thanaboripat, D., 2002. Importance of aflatoxins, KMITL Science Journal, 2(1), 38-45.
[14] Mondali, N.K., Mojumdar, A., Chatterje, S.K., Banerjee, A., Datta, J.K. and Gupta, S., 2009. Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium, Journal of Applied Science and Environmental Management, 13(1), 49-53.
[15] Campbell, R., 1989. Biological Control of Microbial Plant Pathogens. New York,Cambridge University Press.
[16] Calistru, C., Mclean, M. and Berjak, P., 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichodema species. I.Macroscopial and microscopical observations of fungal interactions, Mycopathologia, 139,115-121.
[17] Santamarina, M.P., Roselló, J., Llacer, R. and Sanchis, V., 2002. Antagonistic activity of Penicillium oxalicum Corrie and Thom, Penicillium decumbens Thom and Trichoderma harzianum Rifai isolates against fungi, bacteria and insects in vitro, ReviewIberoam Micology, 19, 99-103.
[18] Thanaboripat, D., Sappakitjanon, N., Prommi, L. and Chareonsettasilp, S., 2009. Screening of fungi for the control of Aspergillus parasiticus, KMITL Science and Technology Journal,9(2), 95-102.
[19] Wongsariya, K., Kuntonwan, S. and Sangvorawanna, S., 2005. Screening of Trichoderma spp. for Inhibition of Aflatoxin Producing Fungi. A Special Project. Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
[20] Nguefack, J., Leth, V., Zollo, A.P.H. and Mathur, S.B., 2004. Evaluation of five essential oils from aromatic plant of Cameroon for controlling food spoilage and mycotoxin producing fungi, International Journal of Food Microbiology, 94, 329-334.
[21] Calistru, C., Mclean, M. and Berjak, P., 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species: A study of the production of extracellular metabolites by Trichoderma species, Mycopathologia, 137,115-124.
[22] Vizcaino, J.A., Sanz, L., Basilio, A., Vicente, F., Gutierrez, S., Hermosa, M.R. and Monte,E., 2005. Screening of antimicrobial activities in Trichoderma isolate representing three Trichoderma sections, Mycological Research, 109(12), 1397-1406.
[23] Se-Gul, J., Kyung-Su, J., Eun-Hee, L., Won-Sik, K. and Jae-Yong, C., 2009. Isolation of 1’,3’- Dilinolenoyl-2’-Linoleoylglycerol with tyrosinase inhibitory activity from Flammulina velutipes, Journal of Microbiology and Biotechnology, 19(7), 681-684.
[24] Lorian, V., 1986. Antibiotics in Laboratory Medicine. Philadelphia, Lippincott Williams&Wilins.
[25] Jantarach, J. and Thanaboripat, D., 2010. The efficacy of ethyl acetate extract of Trichoderma culture broth on growth inhibition and aflatoxin production by Aspergillus flavus IMI 242684, KMITL Science and Technology Journal 10(1), 19-29.
[26] Yin, G., Wang, W., Sha, S., Liu, L. and Yu, X., 2010. Inhibition and control effects of the ethyl acetate extract of Trichoderma harzianum fermented broth against Botrytis cinerea,African Journal of Microbiology Research, 4(15), 1647-1653.
[27] Febles, C.I., Arias, A., Gil-Rodriguez, M.C., Hardisson, A. and Sierra Lopez, A., 1995. In vitro study of antimicrobial activity in algae (Chrorophyta, Phaeophyta and Rodophyta) collected from the coast of Tenerife, Anuario del Instituto de Estudios Canarios, 34, 181-192.
[28] Luckner, M., 1990. Secondary Metabolism in Microorganisms, Plants and Animals. 3rd ed. Springer-Verlag, Berlin.
[29] Yang, L., Xie, J., Jiang, D., Fu, Y., Li, G. and Lin, F., 2008. Antifungal substances produced by Penicillium oxalicum strain PY-1-potential antibiotics against plant pathogenic fungi. World Journal of Microbiological Biotechnology, 24, 909-915.
[30] Ma, Y., Chang, Z-Z., Zhao, J-T., and Zhou, M-G., 2008. Antifungal activity of Penicillium stratisporum Pst10 and its biocontrol effect on Phytophthora root rot of chilli pepper,Biological Control, 44(1), 24-31.