The Genetic Characterization of Pseudomonas strains Isolated from Aquatic Animals Based on the 16S-23S rRNA Intergenic SpacerRegions
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Abstract
Pseudomonas species are being used as a biological control, probiotics and bioremediation tools.Nevertheless, phenotypic properties of pseudomonads are the most heterogeneous among the species. Consequently, a thorough characterization of this genus is needed for accurate identification and may lead to a better understanding of the diversity of this genus. Here, 16S-23S rRNA internal spacer regions (ITS1) of Pseudomonas isolated from various aquatic animals were analyzed. PCR amplification of ITS1 region of the studied isolates generated one, two or three bands ranging from 384 bp to 705 bp. The analysis of the DNA products revealed that two genes namely tRNAIle and tRNAAla were detected in all six studied strains. Phylogenetic analysis using the unweighted pair group method of clustering (UPGMA) revealed 3 phylogenetic clusters including Pseudomonas aeruginosa, P.putida and P. fluorescens. This report for the first time suggests that there was intercistronic heterogeneity of the ITS1 among the pseudomonad strains in aquaculture environment in Thailand.
Keywords: Pseudomonas, 16S-23S rRNA, ITS1, aquatic animal
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