Exploitation of Aqueous Plant Extracts for Reduction of Fungal Growth and Detoxification of Aflatoxins
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Abstract
Aflatoxins are secondary metabolites of the molds Aspergillus flavus and Aspergillus parasiticus which can grow on a wide variety of agricultural commodities, known to be potent toxic and carcinogenic to test animals. Recent evidences indicate that these toxins may also be involved in the etiology of human liver cancer in certain parts of the world. To remove these aflatoxins from food and feed, the use of plant extracts for their anti-microbial and antifungal properties have been a subject of wide interest. Regarding the low phytotoxicity, more systemically and easily biodegradable nature, the antifungal products from higher plants may afford as the most suitable source for the development of effective fungicide. The selected plant extracts include Terminalia chebula (Karakkayalu), Solanum xanthocarpum (Mullavanga), Syzygium cumini (Neredu),Capsicum annuum (Mirapakayalu), Ipomoea mauritiana (Nelagummadi). Results indicate that only S. xanthocarpum fruit extract showed inhibitory property against aflatoxin production. Terminalia chebula and Capsicum annuum extracts also inhibited toxin production of A. parasiticus by 54%, 60% and of A. flavus by 45%, 52%, respectively. Plants having capacity for inhibiting aflatoxin did not exert identical effects on the growth of A. flavus and A. parasiticus. This is evident that whole plant extract of Ipomoea mauritiana only inhibited the growth by 73% and toxin inhibition by 30%. Fruit extracts inhibited the growth by 50% or more and there was no correlation between the inhibition of growth and aflatoxin production. The results show that some plants possess antifungal, antitoxic properties and that aflatoxin production is significantly reduced in some cases.
Keywords: Aflatoxin, Detoxification, Aqueous plant extracts, A. flavus, A. parasiticus.
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References
[2] Richard, J. 1990. Romer TM Labs. Guide to Mycotoxins. Vol 1. Mycotoxins-an overview, U. S. A.
[3] United Nations Food and Agricultural Organization (FAO), World Health Organization,Geneva, 2002. Evaluation of Certain Mycotoxins in Food. Fifty-sixth Report of the Joint FAO/WHO Expert Committee on Food Additives, World Health organization, Geneva.
[4] Ioannou-Kakouri, L., Aletrali, A., Christou, E., Hadjioannou-ralli, A., Koliou, A. and Akkelidou, D. 1999. Surveillance and control of aflatoxins B1, B2, G1, G2, and M1 in foodstuffs in the Republic of Cyprus: 1992-1996. J. AOAC. 82,883- 892.
[5] Ito, Y., Peterson, S. W., Wicklow, D. T. and Goto, T. 2001. Aspergillus pseudotamarii a new aflatoxin producing species in Aspergillus section. Flavi. Mycol. Res., 105, 233-239.
[6] Kurtzman, C. P., Horn, B. W. and Hesseltine, C. W. 1987. Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus parasiticus. Antonie van Leeuwenhoek, 53, 147-158.
[7] Peterson, S. W., Ito, Y., Horn, B. W. and Goto, T. 2001. Aspergillus bombycis, a new aflatoxigenic species and genetic variation in its sibling species, A. nomius. Mycologia, 93,689-703.
[8] Sun, Zeng-Mei and Qi. Zu-tong. 1991. A new aflatoxin producing species of Sect. Flavi of Aspergillus. Acta Mycolog. Sin10, 22-26.
[9] El-kady, I., Maraghy, S. El. and Zihri, A. 1994. Mycotoxin producing potential of some isolates of Aspergillus flavus and Europium groups from meat products. Microbiol. Res., 149,297-307.
[10] Saito, M. and Tsuruta, O. 1993. A new variety of Aspergillus flavus from tropical soil in Thailand and its aflatoxin productivity. Proc. Jpn. Assoc. Mycotoxicol, 37, 31-36.
[11] Waal beek, W., Scott, P. M. and Thatcher, F. S. 1968. Mycotoxins from some food-borne fungi. Can. Microbiol J, 14,131-137.
[12] Hesseltine, C. W. 1973. Recent reports for the control of mycotoxins in cereals. Pure App. Chem, 35, 251.
[13] Mojatahedi, H., Danesh, D., Haghighi, B. and Barnett, R. 1974. Post-harvest pathology and mycotoxin contamination of Iranian pistachio nuts. Phytopathol. 68(12), 1800-1804.
[14] Shank, R. C., Goudan, J. E., Wogan, G. N., Nondasuta, A. and Subhamani, B. 1973. Directory aflatoxin and human liver cancer. Food Cosmetics Toxicol.10, 71.
[15] Schatzmayr, G., Zehner, F., Täubel, M., Schatzmayr, D., Klimitsch, A., Loibner, A. P. and Binder, E. M. 2006. Microbiologicals for deactivating mycotoxins. Mol. Nutr. Food. Res, 50, 543-551.
[16] Bhatnagar, D., Lillehoj, E. B. and Bennett, J. W. 1991. Biological detoxification of mycotoxins. In: Smith JE, Henderson, RS editors. Mycotoxins and Animal Foods. 816-826. CRCPress, Boston.
[17] Bata, A. and Lásztity R. 1999. Detoxification of mycotoxin-contaminated food and feed by microorganisms. Trends Food Sci. Technol, 10, 223-228.
[18] Mishra, H. N., Chitrangada, D. 2003. A review on biological control and metabolism of aflatoxin. Crit. Rev. Food Sci. Nutr., 43(3), 245-264.
[19] Dorner, J. W. 2004. Biological control of aflatoxin contamination of crops. J Toxicol - Toxin Rev, 23(2&3), 425-450.
[20] Shetty, P. H. and Jespersen, L. 2006. Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends Food Sci. Technol, 17, 48-55.
[21] Grabarkiewicz-Saczesna, J., Golinski, P., Chelkowski, P. J. and Szebiotko, K. 1985. Mycotoxins in cerals grain, Part XI, Simple multidetection procedure for determination of 11 mycotoxins in cereals. Nahrung Food, 3, 229-240.
[22] Bokhari, F. M. and Shaker, S. A. 2008. Ochratoxin A contaminated Arabian coffee seeds: effects on liver, kidney functions and structure in mice: Role of roasting and Vitamin C. Advances Biol. Res, 2(1-2), 17-25.
[23] Dos Santos, V. M., Dorner, J. W. and Carrira, F. 2003. Isolation and toxigenicity of Aspergillus fumigatus from moldy silage. Mycopathologia, 156(2), 133-138.
[24] Roberts, B. A. and Patterson, D. S. P. 1984. Detection of twelve mycotoxins safety. New York, NY: Marcel Dekker (1), 279-306.
[25] El-Shanawan, A. A., Mustafa, M. E. and Barakat, A. 2005. A Fungal populations and mycotoxins in silage in assuit and sohag governorates in Egypt, with special reference to characteristic Asperigilli toxins. Mycopathologia, 1598(2), 281-289.
[26] Wogan, G. N. and Goldblatt, L. A. 1969. Aflatoxins. Academic Press, New York.
[27] Singh, O. M. and Singh, T. P. 2010. J. Scientific Industrial Research ,69, 732-740
[28] Fewell, A. M., Roddick, J. G. and Weissenberg, M. 1994. Phytochemistry, 37, 1007-1011
[29] Dixit, S. L. and Misra, S. B. 1977. Screening of some medicinal plants for anti-fungal activity. Geobios (4), 129-132.
[30] Tripathi, S. C. and Dixit, S. N. 1977. Fungitoxic properties of Rosa chinesis Jacq. Experientia. 33, 207.
[31] Misra, P. R. S., Sinha, K. K. and Bilgrami, K. S. 1979. Effect of aqueous plant extracts on aflatoxin production and growth of A. parasiticus. Bio.Bull.India (1), 35-42.
[32] Misra, S. B. and Dixit, S. N. 1978. Antifungal properties of leaf extracts of Ranunculus scleratus. L. Experientia 34, 1442-1443.
[33] Reddy, K. R. N., Raghavender, C. R., Reddy, B. N. and Salah, B. 2010. African J. Biotechnology, 9 (27), 4247-4250.
[34] Reddy, K. R. N., Reddy, K. and Muralidharan, K. 2009. Food Control 20, 173-178.
[35] Safara, M., Zaini, F., Hashemi, S. J., Mahmoudi, M., Khosravi, A. R. and Shojai-Aliabadi, F. 2010. Iranian J. Publ Health 39(2), 24-29.
[36] Fardos Bokhari and Magda Mohammad Aly. 2009. African J. Food Science, 3(3), 068-076.
[37] Frisvada, J. C., Skouboeb, P. and Robert, A. S. 2005. Systematic and Applied Microbiology,28, 442-453.
[38] Sandoz Kumar, R., Karthikeyan, M. S., Mathiyazhagan, Mohan Kumar, G., Chandrasekar and Velazhahan, R. 2007. World J. Microbiol. Biotechnol. 27 (7), 34-47
[39] Sharmila Banu,G., Kumar, G. and Murugesan, A. G. 2009. Indian J. Clinical Biochemi. 24(4), 414-418.