Application of Non-Thermal Processing for Preservation of Orange Juice

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Jarupan Kuldiloke
Mohammad Naghi Eshtiaghi*

Abstract

The application of non-thermal processing for inactivation of yeast (Saccharomyces cerevisiae) in orange juice was investigated and compared with conventional thermal processing. The results showed that less than 1 log of yeast in orange juice could be inactivated during thermal processing at 50 0C and 15 min treated time. In contrast high pressure treatment at moderate temperature (50°C) and 100 to 300 MPa resulted in total (up to 6 log ) inactivation of yeast in orange juice. Supercritical CO2/liquid CO2 treatment at ≥6 MPa and 50 °C was sufficient to inactive yeast up to 6 log after 15 min treatment time. For high electric field pulses, the field strength used in this study(15 kV/cm) was high enough to reduce the yeast number up to 2 log after 60 pulses in orange juice.Inactivation of yeast in orange juice using ultrasound is possible. Ultrasound amplitude at ≥40%(≥80 W) combined with moderate temperature (50 0C) resulted in up to 6 log inactivation of yeast in orange juice after 15 min treatment.


KEYWORDS: non-thermal processing, orange juice, yeast inactivation, ultrasound


E-mail: egmne@mahidol.ac.th

Article Details

Section
Original Research Articles

References

[1] Raso, J., Calseron, M. L., Gongora, M., Barbos-Canovas, G. and Swanson, B. G. 1998 Inactivation of mold ascospores and conidiospores suspended in fruit juices by pulsed electricfields, Journal of Food Science and Technology (LWT), 31, 668–672.
[2] Arnsson. K., Lindgren, M., Johansson, B. R. and Ronner, U. 2001 Inactivation of microorganisms using pulsed electric fields: the influence of process parameters on Escherichia coli, Listeria innocua, Leuconostoc mesenteroides and Saccharomyces cerevisiae, Innovative Food Science & Emerging Technologies, 2, 41–54.
[3] Sale, A. J. H. and Hamilton, W. A. 1968 Effect of high electric fields on microorganisms III. Lysis of erythrocytes and protoplasts, Bioghim Biophys Acta 163, 37–43.
[4] Raso, J. and Heinz, V. 2006 Pulsed Electric Fields Technology for the Food Industry. Springer.
[5] Hoover, D. G., Metrick. C., Papineau, A. M. Farkas, D. F. and Knorr, D. 1989 Biological effects of high hydrostatic pressure on food microorganism. Food Technology, 43(3), 99–107.
[6] Morita, R. Y. 1975. Psychrophilic Bacteria, Bacteriological Review, 39, 144–167.
[7] Ballestra, P., Silva, A. A. D.and Cuq, J. L. 1996. Inactivation of Escherichia coil by carbon dioxide under pressure, Journal of Food Science, 61, 829–836.
[8] Erkmen, O. 1997. Antimicrobial effect of pressurized carbon dioxide on Staphylococcus aureus in broth and milk, Lebensm. Wiss. u. Technology, 30, 826–829.
[9] Hass, G. J., Prescott, H. E., Dudley, E., Dik, R., Hintlian, C. and Keane, L. 1989. Inactivation of microorganisms by carbon dioxide under pressure, Journal of Food Safety, 9, 253.
[10] Kamihira, M., Taniguchi, M. and Koybsyashi, T. 1987. Sterilization of microorganisms with supercritical carbon dioxide, Agricultural Biological Chemistry, 2, 407.
[11] Truong, J. M., Boff, D. B. and Shellhamer, T. H. 2002. Effects of carbon dioxide in highpressure processing on pectin methylesterase in single strength orange juice., Journal of Food Science 67 (8), 3058–3062.
[12] Tedjo, W., Eshtiaghi, M. N. and Knorr, D. 2000. Impact of supercritical carbon dioxide and high pressure on lipoxygenase and peroxidase activity, Journal of Food Science, 65 (8), 1284–1287.
[13] Suslik, K. S. 1988. Ultrasounds: its Chemical, Physical and Biological Effects, New York,VHC Publishers.
[14] Lillard, H. S. 1994. Decontamination of poultry skin by sonication, Food Technology, 48, 72–73.
[15] Sala, F.J., Burgos, J., Condón, S., López, P. and Raso, J. 1995. Effect of heat and ultrasound on microorganisms and enzymes. In New Methods of Food Preservation, pp. 176–204. G. W.Gould (ed.). Blackie Academic and Professional.
[16] Toepfel, S., Heinz, V. And Knorr, D. 2007. High intensity pulsed electric fields applied for food preservation, Chemical Engineering and Processing, 46, 537–546.
[17] Spilimbergo, S., Mantonan, D. and Dalser, A. 2007. Supercritical gases pasteurization of apple juice. Journal of Supercritical Fluids, 40, 485–489.
[18] Parton, T., Elvassore, N., Bertucco, A. and Bertoloni, G. 2007. High pressure CO2 inactivation of food: A multi-batch reactor system for inactivation kinetic determination, Journal of Supercritical Fluids, 40, 490–496.
[19] Chen, C. and Tseng, C-W. 1996. Effect of high hydrostatic pressure on the temperature dependence of Saccharomyces cerevisiae and Zygosaccharomyces rouxii. Process Biochemistry, 32 (4), 337–343.
[20] Raso J, Pagan R, Condón S, Sala J. F. 1998. Influence of the temperature and pressure on the lethality of ultrasound, Applied and Environmental Microbiology; 64, 465–471.
[21] Berliner, S. 1984. Application of ultrasonic processors, International Biotechnology Laboratory, 2, 42.
[22] Guerrero, S., López-Malo, A. and Alzamora, S. M. 2001. Effect of ultrasound on the survival of Saccharomyces cerevisiae: Influence of temperature, pH and amplitude, Innovative Food Science & Emerging Technologies, 2, 31–39.