Screening of Fungi for the Control of Aspergillus parasiticus
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Published:
Mar 30, 2018
Keywords:
Trichoderma, Aspergillus parasiticus, aflatoxin, biocontrol
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Abstract
Fungal isolates previously selected from soil [KMC 5, SRS 4, 480512 I08 (1) and 480519 I06 (3)] were screened for their ability to produce antifungal substances against aflatoxin producing Aspergillus parasiticus IMI 102566. The production of antifungal substances by these isolates was investigated in yeast extract sucrose broth under static condition. Culture filtrates of these isolates were subsequently tested against A. parasiticus on PDA. The filtrates of all isolates produced varying degrees of inhibition against A. parasiticus with the highest inhibition zone of 33.75 mm. These isolates were identified as Trichoderma and the other two isolates were Penicillium sp.
Keywords: Trichoderma, Aspergillus parasiticus, aflatoxin, biocontrol
* Corresponding author: Tel: 662-3298000 Fax: 662-3298412
E-mail: ktdusane@kmitl.ac.th
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References
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[31] Bissett, J. 1991b. A revision of the genus Trichoderma. III. Section Pachybasium. Canadian Journal of Botany , 69, 2373-2417.
[32] Bissett, J. 1991c. A revision of the genus Trichoderma. IV. Additional notes on section Longibarchiatum. Canadian Journal of Botany, 69, 2418-2420.
[33] Bissett, J. 1992. Trichoderma atroviride. Canadian Journal of Botany, 70, 639-641.
[34] Liu, R., Yang, Q., Thanaboripat, D. and Thunsukon, P. 2004. Screening of Trichoderma species for biological control activity on Aspergillus flavus. KMITL Science Journal , 4 (1), 81-89.
[35] Reddy, C. S., Reddy, K. R. N., Muralidharan, K. and Mangala, U.N. 2008. Aflatoxigenic Aspergilli and aflatoxin contamination of rice and its management in India. [Online]. Available:www.aspergillus.org.uk/secure/conferences/confabstracts/ posters/ AAA%203rd% 20poster/118.pdf
[36] Reddy, K. R. N., Reddy, C. S. and Muralidharan, K. 2009. Potential of botanicals and biocontrol agents on growth and aflatoxin production by Aspergillus flavus infecting rice grains. Food Control 20, 173-178.
[37] Odebode, A. C. 2006. Control of postharvest pathogens of fruits by culture filtrate from antagonistic fungi. Journal of Plant Protection Research, 46 (1), 1-5.
[38] Ghisalberti, E. I. and Sivasithamparam, K. 1991. Antifungal antibiotics produced by Trichoderma spp. Soil Biology and Biochemistry, 23, 1011-1020.
[39] Haran, S., Schickler, A. and Chet, I. 1996. Differential expression of Trichoderma harzianum chitinases during mycoparasitism. Phytopathology , 86, 980-985.
[40] Benhamou, N. and Chet, I. 1993. Hyphal interaction between Trichodema harzianum and Rhizoctonia solani: Ultrastructure and gold chemistry of the mycoparasitic process. Phytopathology, 83, 1062-1071. KMITL Sci. Tech. J. Vol. 9 No. 2 Jul. - Dec. 2009 102
[41] Gachomo. E. W. and Kotchoni, S. O. 2008. The use of Trichoderma harzianum and T. viride as potential biocontrol agents against peanut microflora and their effectiveness in reducing aflatoxin contamination of infected kernels. Biotechnology, 7, 1-9.
[42] Vey, A., Hoag, I. R. E. and Butt, T. M. 2001. Toxic metabolites of fungal biocontrol agents. In: T. M. Butt, C. Jackson and N. Magan, Ed. Fungi as Biocontrol Agents: Progress,
Problems and Potential. CAB International Publishing, Bristol, p.311.
[43] Benhamou, N. and Cherif, M. 1990. Cytochemical aspects of chitin breakdown during the parasitic action of Trichodema sp. on Fusarium oxysporum f. sp. radicis-lycopersici. Phytopathology , 71, 1406-1414.
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[46] Yang, L., Xie, J., Jiang, D., Fu, Y., Li, G. and Lin, F. 2008. Antifungal substances produced
by Penicillium oxalicum strain PY-1-potential antibiotics against plant pathogenic fungi. World Journal of Microbiological Biotechnology, 24, 909-915.
[47] Ma, Y., Chang, Zhi-zhou, Zhao, Jiang-tao and Zhou, Ming-guo. 2008. Antifungal activity of Penicillium stratisporum Pst10 and its biocontrol effect on Phytophthora root rot of chilli pepper. Biological Control, 44 (1), 24-31.
62, 4096-4038.
[2] Peterson, S. W., Ito, Y., Horn, B. W. and Goto, T. 2001. Aspergillus bombycis, a new aflatoxigenic species and genetic variation in its sibling species, A. nomius. Mycologia, 93,
689-703. KMITL Sci. Tech. J. Vol. 9 No. 2 Jul. - Dec. 2009 100
[3] Wilson, D. M. and Payne, G. A. 1994. Factors affecting Aspergillus flavus group infection and aflatoxin contamination of crops. In: David L. Eaton and John D. Groopman, Ed. The
Toxicology of Aflatoxins. Human Health, Veterinary, and Agricultural Significance. San Diego, Academic Press, Inc., pp. 383-406.
[4] Ito, Y., Peterson, W., Wicklow, D. T. and Goto, T. 2001. Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi. Mycological Research, 105 (2), 233-239.
[5] Carlile, M. J., Watkinson, S. C. and Gooday, G. W. 2001. The Fungi. 2nd ed. San Diego, Academic Press.
[6] Godet, M. and Manaut, F. 2010. Molecular strategy for identification in Aspergillus section Flavi. FEMS Microbiological Letter, 304 (2), 157-168.
[7] Bommakanti, A. S. and Waliyar, F. 2007. Importance of aflatoxins in human and livestock health. [Online]. Available: www.aflatoxin.info/health.asp
[8] Smith, J. E. and Moss, M. O. 1985. Mycotoxins. Formation, Analysis and Significance. Chichester, John Wiley & Sons.
[9] Smith, J. E. 2004. Biotechnology. 4th ed. Cambridge, Cambridge University Press.
[10] Sorenson, W. G, Jones, W., Simpson, J. and Davidson, J. I. 1984. Aflatoxin in respirable airborne peanut dust. Journal of Toxicology and Environmental Health, 14, 525-533.
[11] Montesano, R., Hainaut, P. and Wild, C. P. 1997. Hepatocellular carcinoma: From gene to public health. Review Journal of National Cancer Institute, 89, 1844-51.
[12] Groopmann, J. D. and Thomas, W. K. 1999.CRC Critical Reviews in Toxicology, Chapter 19, pp. 113-124.
[13] Probst, C., Njapau, H. and Cotty, P. J. 2007. Outbreak of an acute aflatoxicosis in Kenya in 2004: Identification of the causal agent. Applied Environmental Microbiology, 73 (8), 2762- 2764.
[14] Lewis, L., Onsongo, M., Njapau, H., Rogers, H. S., Luber, G., Kieszak, S., Nyamongo, J., Backer, L., Dahiye, A. M., Misore, A., DeCock, K., Rubin, C. and the Kenya Aflatoxicosis Investigation Group. 2005. Aflatoxin contamination of commercial maize products during an outbreak of acute aflatoxicosis in Eastern and Central Kenya. Environmental Health ิPerspective, 113, 1763-1767.
[15] Tassaneeyakul, W., Razzazi-Fazeli, E., Porasuphatana, S. and Bohm, J. 2004. Contamination of aflatoxins in herbal medicinal products in Thailand. Mycopathologia, 158, 239-244.
[16] Creppy, E. E. 2002. Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicology Letters, 127, 19-28.
[17] Krishnamurthy, Y. L. and Shashikala, J. 2006. Inhibition of aflatoxin B1 production of Aspergillus flavus, isolated from soybean seeds by certain natural plant products. Letters in Applied Microbiology, 43, 469-474.
[18] Promhirangul, P., Srianujata, S., Charoenkiatkul, S. and Kusamran, W. 1999. Study of aflatoxin content in noodle dishes sold in three areas of Bangkok. M.Sc. Thesis, Graduate School, Mahidol University, Bangkok, Thailand.
[19] Thanaboripat, D. 2002. Importance of aflatoxins. KMITL Science Journal 2 (1), 38-45.
[20] Campbell, R. 1989. Biological Control of Microbial Plant Pathogens. New York, Cambridge University Press.
[21] Calistru, C., Mclean, M. and Berjak, P. 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichodema species. I. Macroscopial and microscopical observations of fungal interactions. Mycopathologia, 139, 115-121. KMITL Sci. Tech. J. Vol. 9 No. 2 Jul. - Dec. 2009 101
[22] Mann, R. and Rehm, H. J. 1976. Degradation products from aflatoxin B1 by Corynebacterium rubrum, Aspergillus niger, Trichoderma viride and Mucor ambiguous. Applied Microbiology and Biotechnology, 2 (4), 297-306.
[23] Dorner, J. W. and Cole, R. J. 2002. Effect of application of nontoxigenic strains of Aspergillus flavus and A. parasiticus on subsequent aflatoxin contamination of peanuts in storage. Journal of Stored Products Research, 38, 329-339.
[24] Santamarina, M. P., Roselló, J., Llacer, R. and Sanchis, V. 2002. Antagonistic activity of Penicillium oxalicum Corrie and Thom, Penicillium decumbens Thom and Trichoderma harzianum Rifai isolates against fungi, bacteria and insects in vitro. Review Iberoam Micology, 19, 99-103.
[25] Wongsariya, K., Kuntonwan, S. and Sangvorawanna, S. 2005. Screening of Trichoderma spp. for inhibition of aflatoxin producing fungi. A Special Project. Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
[26] Calistru, C., Mclean, M. and Berjak, P. 1997. In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichodema species. A study of the production of extracellular metabolites by Trichoderma species. Mycopathologia, 137, 115-124.
[27] Lorian, V. 1986. Antibiotic in Laboratory Medicine. Philadelphia, Lippincott Williams & Wilins.
[28] Rifai, M. A. 1969. A revision of the genus Trichoderma. Mycol. Paper No 116. Commonwealth Mycological Institute, Kew.
[29] Bissett, J. 1984. A revision of the genus Trichoderma. I. Section Longibarchiatum sect. nov. Canadian Journal of Botany, 62, 924-931.
[30] Bissett, J. 1991a. A revision of the genus Trichoderma. II. Infrageneric classification. Canadian Journal of Botany, 69, 2357-2372.
[31] Bissett, J. 1991b. A revision of the genus Trichoderma. III. Section Pachybasium. Canadian Journal of Botany , 69, 2373-2417.
[32] Bissett, J. 1991c. A revision of the genus Trichoderma. IV. Additional notes on section Longibarchiatum. Canadian Journal of Botany, 69, 2418-2420.
[33] Bissett, J. 1992. Trichoderma atroviride. Canadian Journal of Botany, 70, 639-641.
[34] Liu, R., Yang, Q., Thanaboripat, D. and Thunsukon, P. 2004. Screening of Trichoderma species for biological control activity on Aspergillus flavus. KMITL Science Journal , 4 (1), 81-89.
[35] Reddy, C. S., Reddy, K. R. N., Muralidharan, K. and Mangala, U.N. 2008. Aflatoxigenic Aspergilli and aflatoxin contamination of rice and its management in India. [Online]. Available:www.aspergillus.org.uk/secure/conferences/confabstracts/ posters/ AAA%203rd% 20poster/118.pdf
[36] Reddy, K. R. N., Reddy, C. S. and Muralidharan, K. 2009. Potential of botanicals and biocontrol agents on growth and aflatoxin production by Aspergillus flavus infecting rice grains. Food Control 20, 173-178.
[37] Odebode, A. C. 2006. Control of postharvest pathogens of fruits by culture filtrate from antagonistic fungi. Journal of Plant Protection Research, 46 (1), 1-5.
[38] Ghisalberti, E. I. and Sivasithamparam, K. 1991. Antifungal antibiotics produced by Trichoderma spp. Soil Biology and Biochemistry, 23, 1011-1020.
[39] Haran, S., Schickler, A. and Chet, I. 1996. Differential expression of Trichoderma harzianum chitinases during mycoparasitism. Phytopathology , 86, 980-985.
[40] Benhamou, N. and Chet, I. 1993. Hyphal interaction between Trichodema harzianum and Rhizoctonia solani: Ultrastructure and gold chemistry of the mycoparasitic process. Phytopathology, 83, 1062-1071. KMITL Sci. Tech. J. Vol. 9 No. 2 Jul. - Dec. 2009 102
[41] Gachomo. E. W. and Kotchoni, S. O. 2008. The use of Trichoderma harzianum and T. viride as potential biocontrol agents against peanut microflora and their effectiveness in reducing aflatoxin contamination of infected kernels. Biotechnology, 7, 1-9.
[42] Vey, A., Hoag, I. R. E. and Butt, T. M. 2001. Toxic metabolites of fungal biocontrol agents. In: T. M. Butt, C. Jackson and N. Magan, Ed. Fungi as Biocontrol Agents: Progress,
Problems and Potential. CAB International Publishing, Bristol, p.311.
[43] Benhamou, N. and Cherif, M. 1990. Cytochemical aspects of chitin breakdown during the parasitic action of Trichodema sp. on Fusarium oxysporum f. sp. radicis-lycopersici. Phytopathology , 71, 1406-1414.
[44] Bold, H. C., Alexopoulos, C. J. and Delevoryas, T. 1987. Morphology of Plant and Fungi. 4th ed. New York. Harper and Row Publisher.
[45] Pohanka, A. 2006. Antifungal Antibiotics from Potential Biocontrol Microorganisms. Doctor’s dissertation. Swedish University of Agricultural Science.
[46] Yang, L., Xie, J., Jiang, D., Fu, Y., Li, G. and Lin, F. 2008. Antifungal substances produced
by Penicillium oxalicum strain PY-1-potential antibiotics against plant pathogenic fungi. World Journal of Microbiological Biotechnology, 24, 909-915.
[47] Ma, Y., Chang, Zhi-zhou, Zhao, Jiang-tao and Zhou, Ming-guo. 2008. Antifungal activity of Penicillium stratisporum Pst10 and its biocontrol effect on Phytophthora root rot of chilli pepper. Biological Control, 44 (1), 24-31.