cDNA Microarray Technology for the Analysis of Gene Expression
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Abstract
DNA Microarray technology is a new and powerful tool for studying a genome-wide gene expression in many orgamism. This technology is a high throughput, highly parallel RNA expression assay technique that permits quantitative analysis of RNAs transcribed from both known and unknown genes. This review describes array manufacturing, experimental design, and its application to gene discovery and expression. Microarray technology id rapidly becoming a central platform for functional genomics.
Keywords: microarray, gene expression, functional genomics
Corresponding author: E-mail: [email protected]
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References
[2] Liang, P. and Pardee, A.B. 1992 Differential Display of Eukaryotic Messenger RNA by means of the Polymerase Chain Reaction, Science, 257(5072), 967-971.
[3] Schena, M., Shalon, D., Davis, R.W. and Brown, P.O. 1995 Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray, Science, 270(5235), 467-470.
[4] Lipshutz, R.J., Fodor, S.P., Gingeras, T.R. and Lockhart, D.J. 1999 High Density Synthetic Oligonucleotide Arrays, Nat Genet, 21(1 Suppl), 20-24.
[5] Shalon, D., Smith, S.J. and Brown, P.O. 1996 A DNA Microarray System for Analyzing Complex DNA Samples using Two-color Fluorescent Probe Hybridization, Genome Res., 6(7), 639-645.
[6] DeRisi, J., Penland, L., Brown, P.O., Bittner, M.L., Meltzer, P.S., Ray, M., Chen, Y., Su, Y.A. and Trent, J.M. 1996 Use of a cDNA Microarray to Analyse Gene Expression Patterns in Human Cancer, Nat. Gent, 14(4), 457-460.
[7] Lockhart, D.J. and Winzeler, E.A. 2000 Genomics, Gene Expression and DNA Arrays, Nature, 405(6788), 827-836.
[8] Jordan, B.R. 1998 Large-scale Expression Measurement by Hybridization Methods: from High-density Membranes to “DNA”, J. Biochem (Tokyo), 124(2), 251-258.
[9] Whitney, L.W., Becker, K.G., Tresser, N.J., Caballero-Ramos, C.I., Munson, P.J., Prabhu, V.V., Trent, J.M., McFarland, H.F. and Biddison, W.E. 1999 Analysis of Gene Expression in Multiple Sclerosis Lesions using cDNA Microarrays, Ann Neurol, 46(3), 425-428.
[10] Hughes, T.R., Mao, M., Jones, A.R., Burchard, I., Marton, M.J., Shannon, K.W., Lefkowitz, S.M., Ziman, M., Schelter, J.M., Meyer, M.R., Kobayashi, S., Davis, C., Dai, H., He, Y.D., Stephaniants, S.B., Cavet, G., Walker, W.L., West, A., Coffey, E., Shoemaker, D.D., Stoughton, R., Blanchard, A.P., Friend, S.H. and Linsley, P.S. 2001 Expression Profiling using Microarrays Fabricated by an Ink-jet Oligonucleotide Synthesizer, Nat. Biotechnol., 19(4), 342-347.
[11] Tao, H., Bausch, C., Richnond, C., Blattner, F.R. and Conway, T. 1999 Functional Genomics: Expression Analysis of Escherichia coli Growing on Minimal and Rich Media, J. Bacteriol., 18(20), 6425-6440.
[12] Washburn, M.P., Koller, A., Oshiro, G., Ulaszek, R.R., Plouffe, D., Deciu, C., Winzeler, E. and Yates, J.R., 3rd, 2003 Protein Pathway and Complex Clustering of Correlated mRNA and Protein Expression Analyses in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. U S A, 100(6), 3107-3112.
[13] Wang, R., Guegler, K., LaBrie, S.T. and Crawford, N.M. 2000 Genomic Analysis of a Nutrient Response in Arabidopsis Reveals Diverse Expression Patterns and Novel Metabolic and Potential Regulatory Genes Induced by Nitrate, Plant Cell, 12(8), 1491-1509.
[14] Arnold, C.N., McElhanon, J., Lee, A., Leonhart, R. and Siegele, D.A. 2001 Global Analysis of Escherichia coli Gene Expression during the Acetate-induced Acid Tolerance Response, J. Bacteriol., 183(7), 2178-2186.
[15] Gasch, A.P., Huang, M., Metzner, S., Botstein, D., Elledge, S.J. and Brown, P.O. 2001 Genomic Expression Responses to DNA-damaging Agents and the Regulatory Role of the Yeast ATR Homolog Mec1p, Mol. Biol. Cell, 12(10), 2987-3003.
[16] Zheng, M., Wang, X., Templeton, L.J., Smulski, D.R., LaRossa, R.A. and Storz, G. 2001 DNA Microarray-mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide, J. Bacteriol., 183(15), 4562-4570.
[17] Perou, C.M., Jeffrey, S.S., van de Rijn, M., Rees, C.A., Eisen, M.B., Ross, D.T., Pergamenschikov, A., Williams, C.F., Zhu, S.X., Lee, J.C., Lashkari, D., Shalon, D., Brown, P.O. and Botstein, D. 1999 Distinctive Gene Expression Patterns in Human Mammary Epithelial Cells and Breast Cancers, Proc. Natl. Acad. Sci. USA, 96(16), 9212-9217.
[18] DeRisi, J.L., Iyer, V.R. and Brown, P.O. 1997 Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale, Science, 278(5338), 680-686.
[19] Marton, M.J., DeFisi, J.L., Bennett, H.A., Iyer, V.R., Meyer, M.R., Roberts, C.J., Stoughton, R., Burchard, J., Slade, D., Dai, H., Bassett, D.E., Jr., Hartwell, L.H., Brown, P.O. and Friend, S.H. 1998 Drug Target Validation and Identification of Secondary Drug Target Effects using DNA Microarrays, Nat. Med., 4(11), 1293-1301.