Antibacterial Activity of Crude Ethanolic Extracts and Essential Oils of Spices Against Salmonellae and Other Enterobacteria
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Abstract
Crude ethanolic extracts and essential oils of 14 spices including cardamom, cinnamon, clove, coriander, cumin, garlic, ginger, holy basil, kaffir lime leaves and peels, lemongrass, mace, nutmeg, black and white pepper, and turmeric were examined for their antibacterial activity against 20 serotypes of Salmonella and 5 species of other enterobacteria using disk diffusion method as preliminary screening. Of these, 9 crude ethanolic extracts and 11 essential oils were selected to determine the minimum inhibitory concentrations (MICs) using microbroth dilution test. Among all ethanolic extracts, clove extract had the most inhibitory effect on the growth of all bacterial strains tested. Oils of clove and kaffir lime peels exhibited greater antibacterial activity against all tested strains, compared to other spice oils. The oils of cardamom, coriander, and cumin were also potent inhibitors of bacterial growth, showing the lowest MIC of 4.2 μl/ml to most bacterial strains tested. Both oil and ethanolic extract of kaffir lime peels showed greater antibacterial action, compared to the extracts of kaffir lime leaves. In general, inhibitory activity of spice oils was greater than that of their own ethanolic extracts. Of all serotypes of Salmonella tested, Salmonella Typhimurium (non-DT104 strain) is the most susceptible strain to both forms of spice extracts. On the other hand, Salmonella Derby and Salmonella Rissen were the most resistant strains to extracts, followed by Salmonella Agona and Salmonella Typhimurium DT104. Escherichia coli was more susceptible to most of the spice oils than other non-salmonellae strains tested.
Keywords: Salmonella, enterobacteria, spice extract, essential oil, antibacterial activity
Corresponding author: E-mail: knsuree@kmitl.ac.th
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References
[2] Sauer, C.J., Majkowski, J., Green, S. and Eckel, R. 1997 Foodborne Illness Outbreak Associated with a Semi-Dry Fermented Sausage Product, Journal of Food Protection, 60, 1612-1617.
[3] Wallace, D.J., van Gilder, T., Shallow, S., Fiorentino, T., Segler, S.D., Smith, K.E., Shiferaw, B., Etzel, R., Garthright, W.E., Angulo, F.J. and Group, F.W. 2000 Incidence of Foodborne Illness Reported by the Foodborne Diseases Active Surveillance Network (Foodnet)-1997, Journal of Food Protection, 63, 807-809.
[4] Moore, J.E. 2004 Gastrointestinal Outbreaks Associated with Fermented Meats, Meat Science, 67, 565-568.
[5] Pontello, M., Sodano, L., Nastasi, A., Mammina, C., Astuli, M., Domenichini, M., Belluzzi, G., Soccini, E., Silvestri, M.G., Gatti, M., Gerosa, E. and Montagna, A. 1998 A Community-Based Outbreak of Salmonella Enterica Serotype Typhimurium Associated with Salami Consumption in Northern Italy, Epidemiology and Infection, 120(3), 209-214.
[6] Nanasombat, S. 1996 Comparison of Rambach Agar, MSRV Medium and Other Differential Media for Detection of Salmonella in High aw Foods and Low aw Foods. Research Report. Faculty of Science, KMITL, Bangkok.
[7] Nanasombat, S., Prasertsin, V., Graisin, K., Shain, H. and Thanaboripat, D. 2002 Efficacy of New Enzyme-Linked Immunosorbent Assay for Detection of Salmonella in Foods. Government Pharmaceutical Organization Report, Bangkok.
[8] Hirasa, K. and Takemasa, M. 1998 Spice Science and Technology. New York, Marcel Dekker Inc.
[9] Nevas, M., Korhonen, A.-R., Lindtröm, M., Turkki, P. and Korkeala, H. 2004 Antibacterial Efficiency of Finnish Spice Essential Oils against Pathogenic and Spoilage Bacteria, Journal of Food Protection, 67(1), 199-202.
[10] Shelef, L.A. 1983 Antimicrobial Effects of Spices, Journal of Food Safety, 6, 29-44.
[11] Kim, J., Marshall, M.R. and Wei, C. 1995 Antibacterial Activity of Some Essential Oil Components against Five Foodborne Pathogens, Journal of Agricultural and Food Chemistry, 43(11), 2839-2845.
[12] Juven, B.J., Kanner, J., Schved, F. and Weisslowicz, H. 1994 Factors that Interact with the Antibacterial Action of Thyme Essential Oil and its Active Constituents, Journal of Applied Bacteriology, 76, 626-631.
[13] Lachowicz, K.J., Jones, G.P., Briggs, D.R., Bienvenu, F.E., Wan, J., Wilcock, A. and Coventry, M.J. 1998 The Synergistic Preservative Effects of the Essential Oils of Sweet Basil (Ocimum basilicum L.) against Acid-Tolerant Food Microflora, Letters in Applied Microbiology, 26, 209-214.
[14] Elgayyar, M., Draughon, f.A., Golden, D.A. and Mount, J.A. 2001 Antimicrobial Activity of Essential Oils from Plants against Selected Pathogenic and Saprophytic Microorganisms, Journal of Food Protection, 64(7), 1019-1024.
[15] Tassou, C.C., Drosino, E.H. and Nychas, G.J.E. 1995 Effects of Essential Oil from Mint (Mental piperita) on Salmonella enteritidis and Listeria monocytogenes in Model Food Systems at 4˚C and 10˚C, Journal of Applied Bacteriology, 78, 593-600.
[16] Alzoreky, N.S. and Nakahara, K. 2002 Antibacterial Activity of Extracts from Some Edible Plants Commonly Consumed in Asia, International Journal of Food Microbiology, 80, 223-230.
[17] Swetwiwathana, A., Leutz, U., Lotong, N. and Fischer, A. 1999 Controlling of the Growth of Salmonella anatum in Nham, Fleischwortschaft, 9, 124-128.
[18] Jorgensen, J.H., Turnidge, J.D. and Washington, J.A. 1999 Antibacterial Susceptibility Tests: Dilution and Disk Diffusion Methods. In: Murray, P.R., Barron, E.J., Praller, M.A., Tenover, F.C. and Yolken, R.H., Eds. Manual of Clinical Microbiology. Washington, D.C. ASM Press, pp. 1526-1562.
[19] Oka, S. 1964 Mechanism of Antimicrobial Effect of Various Food Preservatives. In: Molin, N., Ed. Microbial Inhibitors in Food, The Fourth International Symposium of Food Microbiology, Stockholm, Sweden, Almqvist & Wiksell, pp. 3-16.
[20] de Guzman, C.C. and Siemonsma, J.S. 1999 Plant Resources of South-East Asia No 13: Spices. Bogor, Indonesia, Prosea Foundation.
[21] Beuchat, L.R. and Golden, D.A. 1989 Antimicrobials Occurring Naturally in Foods, Food Technology, 43(1), 134-142.
[22] Suksringam, B. 1975 Antiseptic Properties of Some Spices. M.S. Thesis. Kasetsart University, Bangkok.
[23] Farag, R.S., Daw, Z.Y., Hewed, F.M. and El-Baroty, G.S.A. 1989 Antimicrobial Activity of Some Egyptian Spice Essential Oils, Journal of Food Protection, 52(9), 665-667.
[24] Lawrence, B.M., Hogg, J.W., Terhune, S.T. and Podimuang, V. 1970 The Leaf and Peel Oils of Cirtus hystrix DC. Bangkok, ASRCT.
[25] Uribe, S., Ramirez, J. and Peña., A. 1985 Effect of β-pinene on Yeast Membrane Functions, Journal of Bacteriology, 131, 1195-1200.
[26] Ashurst, P.R. 1991 Food Flavourings. Scotland, Blackie and Son Ltd.
[27] Farrell, K.T. 1990 Spices, Condiments, and Seasonings. 2nd ed., New York, Van Nostrand Reinhold.
[28] Arora, D.S. and Kaur, J. 1999 Antimicrobial Activity of Spices, International Journal of Antimicrobial Agents, 12, 257-262.
[29] Conner, D.E. 1993 Naturally Occurring Compounds. In: Davidson, P.M. and Branen, A.L, Eds. Antimicrobials in Foods. New York, Marcel Dekker Inc., pp. 441-468.
[30] Johnson, M.G. and Vaughn, R.H. 1969 Death of Salmonella typhimurium and Escherichia coli in the Presence of Freshly Reconstituted Dehydrated Garlic and Onion, Applied Microbiology, 17, 903-905.
[31] Saleem, Z.M. and Al-Delaimy, K.S. 1982 Inhibition of Bacillus cereus by Garlic Extracts, Journal of Food Protection, 45(11), 1007-1009.
[32] Chaisawadi, S., Thongbutr, D., Methawiriyasilp, W., Chaisawadi, A., Pitakworarat, N., Jaturonrasamee, K., Khemkhaw, J. and Tanuthumchareon, W. 2003 Preliminary Study of Antimicrobial Activities on Medical on Herbs of Thai Food’s Ingredients. The Third World Congress on Medicinal and Aromatic Plants. Chiang Mai, Thailand.
[33] Sikkema, J., de Bont, J A.M. and Poolman, B. 1995 Mechanism of Membrane Toxicity of Hydrocarbons, Microbiological Reviews, 59(2), 201-222.
[34] Wilkins, K.M. and Board, R.G. 1989 Natural Antimicrobial Systems. In: Gould, G.W., Ed. Mechanisms of Action of Food Preservation Procedures. London, Elsevier, p. 285.
[35] Leistner, L. 1999 Combined Methods for Food Preservation. In: Rahman, M.S., Ed. Handbook of Food Preservation. New York, Marcel Dekker, pp. 457-485.