Chemical Compounds, Anticancer and Antioxidant Activities of Volatile Oil from Piper sarmentosum Roxb., Polyscias fruticose Harms. And Polygonum odoratum Lour.

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Published: Mar 30, 2018
Keywords:
GC-MS, Volatile oil, Piper sarmentosum Roxb., Polyscias fruticose Harms., Polygonum odoratum Lour., Anticancer, Antioxidant

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Rungthip Kawaree*
Pharmaceutical Sciences Department, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand.
Weerachai Phutdhawong
Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.
Porntipa Picha
The National Cancer Institute, Department of Medical Services, Ministry of Public Health, Bangkok, Thailand.
Jarunya Ngamkham
The National Cancer Institute, Department of Medical Services, Ministry of Public Health, Bangkok, Thailand.
Sombat Chowwanapoonpohn
Pharmaceutical Sciences Department, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand.

Abstract

The volatile oils from fresh leaves of Piper sarmentosum Roxb., Polyscias fruticose Harms. and Polygonum odoratum Lour. Were isolated by hydrodistillation and analysed through a combination of gas chromatography-mass spectrometry (GC-MS). The essential oils were obtained in 0.0124, 0.1384 and 0.0567% yield, respectively. All volatile oils exist as a yellow liquid. The major constituents of Piper sarmentosum Roxb. were β-caryphyllene (42.51%), α-selinene (11.27%), (+)-β-selinene (11.24%), β-elemene (10.85%) and α-humulene (6.99%). The identified constituents of Polyscias fruticose Harms. include α-bergamotene (20.25%), γ-elemene (15.28%), and germacrene d (13.29%). Two peaks with relative areas of 25.19% and 14.53%, respectively remained unidentified. The major constituents of Polygonum odoratum Lour. Were dodecanal (39.54%), decanal (21.14%), β-caryphyllene (10.10%), 1-tetradeconal (8.84%) and euparone (7.36%). In addition, the volatile oils from Polyscias fruticose Harms. and Polygonum odoratum Lour. Showed significant pharmacological effect (ED50 << 30µg/ml) to anticancer activity, while Piper sarmentosum Roxb. oil exhibited only weak cytotoxic effect (ED50 > 30 µg/ml), from primary screening tests with P388 (mouse lymphocytic leukemia) cells. Moreover, none of the components exhibited antioxidant activity (IC50 values of >> 100µg/ml) in a DPPH radical scavenging assay.


Keywords: GC-MS, Volatile oil, Piper sarmentosum Roxb., Polyscias fruticose Harms., Polygonum odoratum Lour., Anticancer, Antioxidant


Corresponding author: E-mail: t_chem@hotmail.com

Article Details

Issue
Vol. 6 No. 2b (2006)
Section
Original Research Articles

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