Determination of Microcystin LR from Blue-Green Algae (Microcystis aeruginosa Kutz.) Using Reversed-Phase High Performance Liquid Chromatography

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Published: Mar 30, 2018
Keywords:
Microcystis aeruginosa Kutz, reversed-phase HPLC, Microcystin LR, NMR spectroscopic technique

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Weerachai Phutdhawong*
Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.
Siwalee Rungrattanakhun
Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.
Waya Sengpracha
Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon pathom, Thailand.
Napattarapong Suvannachai
Department of Chemistry, Faculty of Science, Maejo University, Chiang Mai, Thailand.

Abstract

Microcystin-LR in Microcystis aeruginosa Kutz. collected from Sri Sakhett, Thailand was extracted and quantitatively analyzed using reversed-phase HPLC with UV detection at 238 nm. The HPLC analysis employed a C-18 column (4.6x125 mm., 5 micron), with methanol-water (60 : 40) at flow rate of 1.0 ml/min as the mobile phase. A linear calibration curve was obtained out using five concentrations in the range from 1 to 5 ppm (r2 =0.9919). A triplicate analysis of 0.5 g of sample yielded 2.93x10-4  3.68 x 10-5 g of microcystin-LR (5.85x10-2 7.35 x 10-3 % yield). NMR spectroscopy was also used to confirm the structure of the microcystin-LR standard.


Keywords: Microcystis aeruginosa Kutz, reversed-phase HPLC, Microcystin LR, NMR spectroscopic technique


Corresponding author: E-mail: weerachai@mju.ac.th

Article Details

Issue
Vol. 6 No. 2b (2006)
Section
Original Research Articles

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