Factors of Callus and Combination of Plant Growth Regulators Effect On the Plant Regeneration from Scutellum of Indica Rice (Oryza sativa L. cv. KDML 105)
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Abstract
Factors effect on the plant regeneration of indica rice (Oryza sativa L. cv. KDML 105), callus age, desiccation, and combination of plant growth regulator, were investigated in this experiment. Compact and friable calli developed from scutellum cultured on NB basal medium containing 500 mg/l L-proline, 500 mg/l L-glutamine, 300 mg/l casein hydrolysate, 2 mg/l 2,4-D and 30 g/l sucrose. Treatments of four-week-old calli and partial desiccation before transferring clumps of embryogenic calli onto NBR medium showed higher rate of green bud and shoot regeneration than that of eight-week-old calli and non desiccation treatments. The adjustment of BA concentration in combination with 1 mg/l IAA was performed. Maximum shoot bud formation frequency (18.92%) was obtained in NBR medium containing 5 mg/l BA. Regeneration shoots were transferred to normal NB medium and planets were successfully grown in the green house.
Keywords: Indica rice, KDML 105, regeneration, callus age, partial desiccation, plant growth regulator
Corresponding author: E-mail: kskanokp@kmitl.ac.th
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