Preliminary Studies on the Creation of Anatomical Changes by Colchicine Treatment in Chinese Kale (Brassica alboglabra L.H. Bailey)

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Published: Mar 30, 2018
Keywords:
colchicine, Chinese kale, anatomical variants, polyploidization

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M. Teerarak*
Department of Horticulture, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology LadKrabang, Bangkok, Thailand.
S. Thitavasanta
Department of Horticulture, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology LadKrabang, Bangkok, Thailand.
S. Khamchutr
Applied Biology Program, Faculty of Science and Technology, Rambhai Barni Rajabhat University, Chantaburi, Thailand.

Abstract

Seeds of Chinese kale were treated with 0, 0.125, 0.25, 0.50, 1 and 2% colchicine for 6, 12 and 24 hours in order to form polyploidy plants and were sown in glass Petri dishes. The results showed that treatments of seeds with all colchicine concentrations had no effect on seed germination and colchicine did induce abnormality in the seedlings. In the continuous experiment, seeds of Chinese kale were soaked in 0, 0.1, 0.2, and 0.4% colchicine solutions for 12 hours and were grown in a greenhouse. The resulted indicated that the stomatal sizes both on the upper and lower epidermis of colchicine treated plant were significantly larger than those of the normal plants while the frequencies of the stomata were reduced significantly.


Keywords: colchicine, Chinese kale, anatomical variants, polyploidization


Corresponding Author Email: noomonmon@hotmail.com

Article Details

Issue
Vol. 6 No. 2b (2006)
Section
Original Research Articles

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References

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