Preliminary Studies on the Creation of Anatomical Changes by Colchicine Treatment in Chinese Kale (Brassica alboglabra L.H. Bailey)

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M. Teerarak*
S. Thitavasanta
S. Khamchutr


Seeds of Chinese kale were treated with 0, 0.125, 0.25, 0.50, 1 and 2% colchicine for 6, 12 and 24 hours in order to form polyploidy plants and were sown in glass Petri dishes. The results showed that treatments of seeds with all colchicine concentrations had no effect on seed germination and colchicine did induce abnormality in the seedlings. In the continuous experiment, seeds of Chinese kale were soaked in 0, 0.1, 0.2, and 0.4% colchicine solutions for 12 hours and were grown in a greenhouse. The resulted indicated that the stomatal sizes both on the upper and lower epidermis of colchicine treated plant were significantly larger than those of the normal plants while the frequencies of the stomata were reduced significantly.

Keywords: colchicine, Chinese kale, anatomical variants, polyploidization

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