The Effect of Molasses for Lysine Production by Arthrobacterium citreus NRRL 1258 and Corynebacterium glutamicum ATCC 21475
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Abstract
The essential amino acid L-lysine was produced in a batch fermentation by Arthrobacterium citreus NRRL 1258 and Corynebacterium glutamicum ATCC 21475. Molass, a food by-product from a sugar mill, was chosen for this study. The main objective was to replace glucose with molasses in order to make use of this by-product and also to reduce total production costs of L-lysine. The results revealed that the growth of Arthrobacterium citreus NRRL 1258 and Corynebacterium glutamicum ATCC 21475 did not follow the simple Monod’s kinetics. Growth inhibition by the product (L-lysine) occurred during fermentation. Substrate (molasses) inhibition at higher concentrations (100, 120 g/l) were exhibited against growth. The concentration of 40 g equivalent sugar/l molasses gave the highest lysine yield of 25.14 g/l for Arthrobacterium citreus NRRL 1258 and of 24.95 g/l for Corynebacterium glutamicum ATCC 21475. In addition, the statistical analysis showed that there was no significant difference of P>0.05 between molasses (40 g/l) and the synthetic glucose (80 g/l). This study clearly indicate that molasses (40 g/l) was a carbon suitable source in the lysine production process.
Keywords: Molasses, Arthrobacterium citreus, Corynebacterium glutamicum, Lysine Production
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References
[2] Collo, N., L. Brito and Nonus, M. 2000. Biosynthesis of L-lysine by Corynebacterium glutamicum Grown on Fish Silage. Bioresource Boitechnology. 73, 2000, 221-225.
[3] Kiefer, E.H. and Wittmann, C. 2002. Influence of glucose, fructose and sucrose as carbon sources on kinetics and stoichinometry of lysine production by Corynebacterium glutamicum, J. Ind. Microbiol. Biotechnol. 28, 338-343.
[4] Umerie, S.C., Ekwealor, I.A. and Nwagbo, I.O. 2003. Lysine production by Bacillus laterosporus from various carbohydrate and seed meals. Bioresource Biotechnology. 75, 249-252.
[5] Gessesse, A.1997. The use of nugmeal as a low-cost substrate for the production of alkaline protease by alkaliphilic Bacillus sp.AR-009 and some properties of the enzyme. Bioresource Technology. 62, 59-61.
[6] Ruklisha. M., Ruklisha, R., Ionina, Paegle, L. and Petrovica, G. 2001. Metabolism and lysine biosynthesis control in Brevibacterium flavum: impact of stringent response. In: Simon J.-P. and Durieux, A., Ed. Applied Microbiology. Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 51-57.
[7] Kimura, K. 2003. Metabolic engineering of glutamate production. In: Thommel, J. and Faurie, R. Ed. Advances in Biochemical Engineering/Biotechtechnology vol. 79, Springer-Verlag, Berlin Heidelberg, pp. 37-57.
[8] Pham, C.B., Galvez, C.F. and Padolina, W.G., 1992. Methionine production by batch fermentation from various carbohydrates. ASEAN Food Journal 7, pp. 34-37.
[9] Kazushige, O. and Charles E. G. 2000. Effect of carbon source on microfiltration of Corynebacterium glutamicum. J. of Membrane Science. 171, 263-271.