Construction of a Binary Vecter Harboring Bt Gene and Its Transfer to Trichoderma harzianum
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Abstract
A binary vertor harboring Bt gene was constructed and was transferred into biocontrol fungus T. harzianum by Agrobacterium tumefaciens-mediated transformation system. Southern blot analysis showed that the Bt gene was integrated into the genome of T. harzianum, and the transformants contained single copies of the gene. The transformation efficiency was 50-160 transformants per 106 spores.
Keywords: Agrobacterium tumefaciens, Bacillus thuringiensis, Trichoderma harzianum, genetic transformation, plasmid construction
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