Construction of a Binary Vecter Harboring Bt Gene and Its Transfer to Trichoderma harzianum
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Abstract
A binary vertor harboring Bt gene was constructed and was transferred into biocontrol fungus T. harzianum by Agrobacterium tumefaciens-mediated transformation system. Southern blot analysis showed that the Bt gene was integrated into the genome of T. harzianum, and the transformants contained single copies of the gene. The transformation efficiency was 50-160 transformants per 106 spores.
Keywords: Agrobacterium tumefaciens, Bacillus thuringiensis, Trichoderma harzianum, genetic transformation, plasmid construction
Corresponding author: E-mail: cast@kmitl.ac.th
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References
[2] P.J.C. Cannon, Prospects and progress for Bacillus thuringiensis-based pesticides. Pest. Sci., 37, 1993, 331-335.
[3] M.G. Obukowicz et al., Integration of the deltaendotoxin gene of Bacillus thuringiensis into the chromosome of root colonizing strains of Peseudomonads using Tn5. Gene 45, 1986, 327-331.
[4] Liu Yunxia et al., Transformation of Bt gene to endophytes of Oryza sativa. Chinese Journal of Biological Control, 15(2), 1995, 70-72.
[5] Yan Peisheng et al, Progress of filamentous fungal genetic engineering. Progress of Biological Engineering, 19(1), 1999, 36-41.
[6] P. Bundock, et al., Transkingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO Journal 14, 1995, 3206-3214.
[7] de Groot et al., Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nature Biotechnology 16, 1998, 839-842.
[8] R.J. Gouka et al., Trnasformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination. Nature Biotechnology 19, 1999, 598-601.
[9] R.O. Abuoden et al., Genetic transformation of Coccidioides immitis facilitated by Agrobacterium tumefaciens. Journal of Infectious Diseases 181, 2000, 2106-2110.
[10] S.F. Covert et al., Agrobacterium tumefaciens-mediated transformation of Fusarium circinatum. Mycol. Res 105(3), 2000, 259-264.
[11] P. Russo et al., Cloning, sequence and expression of a β-tublin-encoding gene in the homobasidiomycete Schizophyllum commune. Gene, 119, 1992, 175-182.
[12] E.D. Mullins et al., Agrobacterium-mediated transformation of Fusariume oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 91, 2000, 173-180.
[13] Q.K. Hoang et al., Agrobacterium tumefaciens-mediated expression of cry genes in Trichoderma harzianum. XXI fungal Genetics Conference, Asilomar, California March 2001.
[14] Q.Yang et al., Study on the transformation of Trichoderma spp. with resistance gene to Carbendazim. Chinese Science Bulletin 43(22), 1998, 2423-2426.