Optimization of Single Cell Protein Production from Cassava Processing Wastewater by Mixed Culture of Endomycopsis fibuligera TISTR 5097 and Candida utilis TISTR 5046
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Abstract
Optimization of single cell protein production from cassava processing wastewater by mixed culture of E. fibuligera TISTR 5097 and C. utilis TISTR 5046 was studied. E. fibuligera TISTR 5097 and C. utilis TISTR 5046 were mixed with a volume ratio of 1 : 4 and corn steep liquor was used as nitrogen source. The results showed that the maximum cell dry weight of mixed culture was 4.89 g/L and protein content was 0.47 g protein/g dry weight at initial pH 4.0 in a shake flask culture at 25 °C and shaking speed of 200 rpm. The optimum agitation speed, aeration rate for the growths of theses yeasts in a fermenter were 300 rpm, 1.5 vvm with cell dry weight of 6.97 g/L
Keywords: single cell protein, cassava processing wastewater, Endomycopsis fibuligera, Candida utilis, optimization.
Corresponding author: E-mail: cast@kmitl.ac.th
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References
[2] A. A. Coleman and B. Ooraikul, Charactertics of pure culture canola source fermentation, J. Food. Proc. Preserv, 13, 1989, 245-256.
[3] P. Pangri and D. Ochaikul, Production of single cell protein from cassava processing wastewater by mixed culture of Endomycopsis fibuligera TISTR 5097 and Candida utilis TISTR 5046, Biothailand 2003 : Technology for Life, 17 – 20 July 2003, Pattaya, Thailand,
[4] O. H. Lowry, M. J. Rosebrough and R. J. Randall, Protein measurement with folin phenol reagent, J. Biol Chem, 193, 1951, 265 – 275.
[5] S. A. Lemmel, R. C. Heimsch and L. I. Edward, Optimizing the continuous production of Candida utilis and Saccharomycopsis fibuligera on potato processing wastewater, Appl. And Environ. Microbiol. 37(2), 1979, 227-232.
[6] Le H. Trien, P. Tasakorn, S. Chavdej, T. Vitidsant and P. Piumsomboon, Production of single cell protein from cassava by mixed culture of Endomycopsis fibuligera TISTR 5097 and Candida utilis TISTR 5001, J. Sci Res Chula Univ., 25, 2000, 137 – 143.
[7] T. Hongpattarakere and A. H – Kittikun, Optimization of single cell protein production form cassava starch using Schwanniomyces castellii, J. Microbiol. Biotech, 11(6), 1995, 607-609.
