Optimization of Single Cell Protein Production from Cassava Processing Wastewater by Mixed Culture of Endomycopsis fibuligera TISTR 5097 and Candida utilis TISTR 5046
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Abstract
Optimization of single cell protein production from cassava processing wastewater by mixed culture of E. fibuligera TISTR 5097 and C. utilis TISTR 5046 was studied. E. fibuligera TISTR 5097 and C. utilis TISTR 5046 were mixed with a volume ratio of 1 : 4 and corn steep liquor was used as nitrogen source. The results showed that the maximum cell dry weight of mixed culture was 4.89 g/L and protein content was 0.47 g protein/g dry weight at initial pH 4.0 in a shake flask culture at 25 °C and shaking speed of 200 rpm. The optimum agitation speed, aeration rate for the growths of theses yeasts in a fermenter were 300 rpm, 1.5 vvm with cell dry weight of 6.97 g/L
Keywords: single cell protein, cassava processing wastewater, Endomycopsis fibuligera, Candida utilis, optimization.
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