Optimal Condition for Chromosomal Imbalances Analysis of Cancer Genome by Comparative Genomic Hybridization

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Published: Mar 30, 2018
Keywords:
chromosome imbalances, comparative genomic hybridization, cancer genome

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Supattar Poeaim
Department of Applied Biology, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
Budsaba Rerkamnuaychoke
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Thailand.
Amara Campiranon
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.

Abstract

Comparative Genomic Hybridization (CGH) is a technique for analysis of copy number changes throughout the genome based on the hybridization of differentially labeled tumor (test DNA) and normal DNA (reference DNA) to normal human metaphase chromosome (target DNA). In this report, we suggested some critical steps such as metaphase preparation and an average fragment length of DNA probe which obtains a uniform and intense hybridization signal. CGH technique will show gain and loss of DNA sequence regions in cancer genome. Those regions may harbor candidate genes such as oncogenes and tumor suppressor genes playing important roles in the initiation and progression of cancer.


Keywords: chromosome imbalances, comparative genomic hybridization, cancer genome


Corresponding author: E-mail: [email protected]


 

Article Details

Issue
Vol. 4 No. 1 (2004)
Section
Original Research Articles

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References

[1] Kallionimie, A., Kallionimie, O – P., Sudar, D., Rutovitz, D., Gray, J.W., Waldman, F.M. and Pinkel, D. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors, Science, 258, 1992, 818-821.
[2] Gebhart, E. and Liehr, T. Patterns of genomic imbalances in human solid tumors (Review), International Journal of oncology, 16(2), 2000, 383-399.
[3] Hermsen, M.A., Meijer, G.A., Baak, J.P., Joenje, H. and Walboomers, J.J. Comparative genomic hybridization: a new tool in cancer pathology, Human Pathology, 27(4), 1996, 342-349.
[4] Kallionimie, O-P., Kallionimie, A., Piper, J., Isola, J., Waldman, F.M., Gray, J.W. and Pinkel, D. Otimizing comparative genomic hybridization for analysis of DNA sequences copy number changes in solid tumors, Gene Chromosomes Cancer, 10, 1994, 231-243.
[5] Karhu, R., Kahkonen, M., Kuukasjarvi, T., Pennanen, S., Tirkkonen, M. and Kallioniemi, O-P. Quality control of CGH: Impact of metaphase chromosomes and the dynamic range of hybridization, Cytometry, 28, 1997, 198-205.
[6] Houldworth, J. and Chaganti, R.S.K. Comparative genome hybridization : an overview, American Journal of Pathology, 145, 1994, 1253-1260.
[7] Ried, T., Knutzen, R., Steinbeck, R., Blegen, H., Schrock, E., Heselmeyer, K., du Manoir, S. and Auer, G. Comparative genomic hybridization reveals a specific pattern of chromosomal gains and losses during the genesis of colorectal tumors, Genes Chromosomes Cancer, 15(4), 1996, 234-245.
[8] Schlegel, J., Stumm, G., Scherthan, H., Bocker, T., Zirngibl, H., Ruschoff, J. and Hofstadter, F. Comparative genomic in situ hybridization of colon carcinomas with replication error, Cancer Research, 55, 1995, 6002-6005.