Production of Cellulase by Trichoderma viride under Different Fermentation Conditions
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Abstract
Trichoderma viride has been widely to produce cellulose. The strain studied has the ability to fix nitrogen and produce ethylene that could reach 1.3×10-6 mol·L-1·d-1. In order to utilize this strain better, the production of different cellulolytic enzymes under different fermentation conditions was studied. The results showed that the optimal ratio of rice straw to wheat bran was 3 to 2 or 1 to 1; the optimal nitrogen source was NH4Cl; the optimal growth temperature was 27-30 °C and the highest production of cellulose enzyme was obtained on the fourth day using solid culture with rice straw and wheat bran. The production of β-glucosidase was very low using liquid medium (Mendel’s), however, the production could be enchanced when 0.2% glucose was added to the culture.
Keywords: cellulose, fixing nitrogen, ferment conditions, β-glucosidase
Corresponding author: E-mail: cast@kmitl.ac.th
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References
[2] Liu Ying, Studies on Screening of Aspergillus niger 9813X8 and Solid Fermentation Conditions, Journal of Hei Longjang August First Reclamation University, 12(4), 2000, 61-65.
[3] R. Triveni and T.R. Shamala Clarification of xanthan gum with extracellular enzymes secreted by Trichoderma koningii, Process Biochemistry, 34, 1999, 49-53.
[4] Fang Jing, Gao Pei-Ji Study on the Role of Cellobiose Dehydrogenase in Cellulose Degradation, Microbiology, 27(1), 2000, 15-18.
[5] Paul Christakopoulos, Dimitris Kekos, Basil J. Macris, Marc Calaeyssens, Mahalingeshwara and K. Bhat, Purification and characterization of a major Xylanas with cellulose and transferase activities from Fusarium oxysporum, Carbohydrate Research, 289, 1996, 91 – 104.
[6] A.L. Abdulah, R.P. Tengerdy and V.G. Murphy, Optimization of solid substrate fermentation of wheat straw. Biotechnol. Bioeng., 5(27), 1985, 20-27.
