Research and Development of Bioengineering Pesticides, Bacillus thuringiensis WG-001
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Published:
Mar 30, 2018
Keywords:
Bacillus thuringiensis, insecticidal cystal protein genes, genetically modified strains, gene expression and regulation
Main Article Content
Abstract
The biopesticides produced by Bacillus thuringiensis have been widely used in the world. On the foundation of exploiting the resource of Bacillus thuringiensis, insecticidal crystal protein genes and helper protein genes were cloned, the appropriate recipient strains were screened, different kinds of vectors were constructed, and expression and regulation of insecticidal crystal protein gene were studies. Environmental safe, highly effective genetically modified Bacillus thuringiensis strains with stable expression have been constructed. Bacillus thuringiensis WG-001 was the first genetically modified pesticide strain which is safety used as a commercial product in China.
Keywords: Bacillus thuringiensis, insecticidal cystal protein genes, genetically modified strains, gene expression and regulation
Corresponding author: E-mail: yz41@mail.hzau.edu.cn
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References
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[23] Cheng Ping, Wang Qingfeng, and Yu Ziniu, The promoters of Bacillus thuringiensis ICP genes and their transcriptional regulation, Microbiology, 26(2), 1999, 130-134.
[24] Cheng Ping, Arthur I. Aronson, and Yu Ziniu, Effect of diferrent crylA promoter upstream mutants in Bacillus thuringiensis on the expression regulation of BtI-I-IacZ fusion, Chinese Journal of Agricultural Biotechnology, 7(4), 1999, 358-362.
[25] Cheng Ping, Wu L, Yu Ziniu, and Arthur I. Aronson, Subspecies-dependent regulation of Bacillus thuringiensis protoxin genes, Applied and Environmental Microbiology, 65(5), 1999, 1849-1853.
[26] Cheng Ping, Arthur I. Aronson, and Yu Ziniu, Study about Bacillus thuringiensis deletional upstream of cry1A promoter, Biotechnology, 10(4), 2000, 3-8.
[27] Zhang Hongyu, Zeng Xiaohui, Yu Ziniu, and Deng Wangxi, Characterization and toxicity of Bacillus thuringiensis isolates from warehouse to Spodoptera exigua (Lep., Noctuidae), Journal of Applied Entomology, 125, 2001, 211-214.
[28] Zhang Lili, Arthur I. Aronson, and Yu Ziniu, Effects of carbon sources on the expression of cry1D-lacZ in Bacillus thuringiensis, Hereditas,24(3), 2000, 301-304.
[29] T.C.Hodgman, Y.u Ziniu, J. Shen, and D.J. Ellar, Identification of a cryptic gene associated with an insertion sequence not preciously identified in Bacillus thuringiensis, FEMS Microbiology Letters, 114, 1993, 23-29.
[30] Sun Ming, Yu Ziniu, Liu Ziduo, and Li Fudi, Modification of Bacillus thuringiensis insecticidal crystal protein gene cry218, Chinese Journal of Biologinal Control, 12(3), 1996, 127-129.
[31] Wang Qingfeng, and Yu Ziniu, Modification of Bacillus thuringiensis insecticidal crystal protein cry1E, Journal of Huazhong Agricultural University, 17(5), 1998, 465-468.
[32] Shao Zongze, Liu Ziduo, and Yu Ziniu, Effects of the 20-Kilodalton helper protein on Cry1Ac production and spore formation in Bacillus thuringiensis, Applied and Environmental Microbiology 67(12), 2001, 5362-5369.
[33] Shao Zongze, Liu Ziduo, and Yu Ziniu, The Effects of 20KDa protein on the expression and crystallization of Cry1A in Bacillus thuringiensis. Biotechnology of Bacillus thuringiensis (Eds. Yu Ziniu, Sun Ming, and Liu Ziduo), 3, 1999, 122-125. Science Press New York.
[34] Lu Songqing, Liu Ziduo, and Yu Ziniu, Transfer of cry1C gene into Bacillus thuringiensis by electroporation to construct strain with broader insecticidal activity, Journal of Biological Engineering, 16(4), 2000, 56-59.
[35] Yue Chaoyin, Liu Ziduo, Li Lin, Jiang Hao, Wei Fang, and Yu Ziniu, Study of insecticidal crystal protein gene cry3A transformant into Bacillus thuringiensis, Journal of Wuhan University (Natural Science Edition), 44, 1998, 51-52.
[36] Yue Chaoyin, and Yu Ziniu, Studies on transformation of transpositive plasmids into Bacillus thuringiensis, Journal of Huazhong Agricultural University, 20(4), 2001, 363-364.
[2] Schnepf, H.E., and Whiteley, H.R., Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli, Proc. Natl Acad. Sci. USA, 78, 1981, 2893-2897.
[3] http://www.biols.susx.ac.uk/home/Neil_Crickmore/B.t/index.html
[4] Sun, M, and Yu.Z, Nucleiotide sequence of cry218 in Bacillus thuringiensis YBT-1520, 1997, Accession number AJ002514.
[5] Liu Ziduo, Li Lin, Sun Ming, and Yu Ziniu, Cloning of Bacillus thuringiensis subsp. israelensis helper protein genes site-directed mutagenesis by PCR, Journal of Wuhan University (Natural Science Edition), 44, 1998, 13-15.
[6] Liu Ziduo, Sun Ming, Yu Ziniu, and A. Zaritsky, Interaction of P19, cytA and 20 kDa protein gene from Bacillus thuringiensis subsp. israelensis, Journal of Agricultural Biotechnology, 5(3), 1997, 292-296.
[7] Liu Ziduo, Sun Ming, Chen Yahua, Yu Ziniu, R. Manassherob, E. Bendov, and A. Zaritsky, The influence of the 20kDa protein from Bacillus thuringiensis subsp. israelensis on the cytolytic activity of CytA, Acta Genetica Sinica, 26(1), 1999, 81-86.
[8] Liu Ziduo, Sun Ming, Yu Ziniu, Zaritsky. A., Ben-Dov E., and Manasherob B., Preliminary study of p19 gene from Bacillus thuringiensis subsp. israelensis, Acta Microbiologica Sinica, 39(2), 1999, 114-119.
[9] Liu Ziduo, Shao Zongze, Zheng Rong, Jiang Hao, Sun Ming, and Yu Ziniu, Effects of the 20kDa protein gene on expression of pesticidal crystal protein gene in wild-type Bt strains. Biotechnology of Bacillus thuringiensis (Eds. Yu Ziniu, Sun Ming, and Liu Ziduo), 3, 1999, 107-112. Science Press. New York).
[10] Zhang Qiong, Sun Ming, Liu Ziduo, and Yu Ziniu, A novel plasmid replication origin from Bacillus thuringiensis YBT-1520, 1999, Accession number AF202532.
[11] Sun Ming and Yu Ziniu, Characteriaztion of a small plasmid in Bacillus thuringiensis YBT-1520, 1998, Acession number AF050161.
[12] Wei Fang, Sun Ming Zhang Qiong, and Yu Ziniu, Three Plasmid’s Replicons cloned from subsp. tenebrionis and subsp. kurstaki, Biotechnology of Bacillus thuringiensis (Eds. Yu Ziniu, Sun Ming, and Liu Ziduo), 3, 1999, 140-141. Science Press. New York.
[13] Wu Lan, Sun Ming, and Yu Ziniu, A new resolution vector with cry1Ac10 gene based on Bacillus thuringiensis transposon Tn4430, Acta Microbiologica Sinica, 14(3), 2000, 264-269.
[14] Sun Ming, Yue Chaoyin, and Yu Ziniu, Construction of integrative vector with Bacillus thuringiensis transposon Tn4430, Chinese Journal of Agricultural Biotechnology, 8(4), 2000, 321-326.
[15] Li Lin, Yang Chao, Liu Ziduo, Li Fudi, and Yu Ziniu, From Bacillus thuringiensis and studies on their transformation properties, Acta Microbiologica, 40(1), 2000, 85-90.
[16] Sun Ming, Dai Jingyuan, Luo Xiaxia, Yu Ling, Liu Ziduo, and Yu Ziniu, Characterization of Bacillus thuringiensis YBT-1520 with high toxicity against Helicoverpa armigera Larvae, Virologica Sinica, 1996, 42-45.
[17] Lu Songqing, Liu Ziduo, Zeng Xiaohui, and Yu Ziniu, The relation between insecticidal crystal protein genes of several Bacillus thuringiensis strains and their toxicity to Spodoptera exigua, Journal of Wuhan University (Natural Science Edition), 1999, (45), 13-16.
[18] Liu M., Sun M., and Yu Z, Bioassay to assess the toxicity of Bacillus thuringiensis to Daikon Leaf Beetle (Coleoperea: Chrysomelidae), Journal of Invertebrate Pathology, 74(3), 1999, 255-260.
[19] Zhang LiLy, Rian Grant, and Arthur Aronson, Regulation of the packaging of, Bacillus –thuringiensis δ-Endotoxins into inclusions, Applied and Environmental Microbioliology, 2001, 5032-5036.
[20] Yu Xiniu, Dai Jinyuan, Chen Yahua, Sun Ming, Lou Xixia, Yu Ling and Liu Ziduo Characterization and toxins of subspecies chinensis with toxicity to house fly larvae (Musca domestica), Proc. VI International Colloquium on Intertebrate Pathology and Microbial Control, Iind International Conf. on Bacillus thuringiensis and XXVI Annual Meeting of the Society for Intertebrate Pathology, Montpellier, France, 1994, 172-176.
[21] T.C. Hodgman, Yu Ziniu, Sun Ming, T.Sawyer, C.M. Nicholls, and D.J. Ellar, Characterization of Bacillus thuringiensis strain which is toxic to the housefly Musca domestica, FEMS Microbiology Letters, 114, 1993, 17-22.
[22] Sun Ming, Liu Ziduo, and Yu Ziniu, Characterization of the insecticidal crystal protein genes of Bacillus thuringiensis YBT-1520, Acta Microbiologica Sinica, 40(4), 2000, 365-371.
[23] Cheng Ping, Wang Qingfeng, and Yu Ziniu, The promoters of Bacillus thuringiensis ICP genes and their transcriptional regulation, Microbiology, 26(2), 1999, 130-134.
[24] Cheng Ping, Arthur I. Aronson, and Yu Ziniu, Effect of diferrent crylA promoter upstream mutants in Bacillus thuringiensis on the expression regulation of BtI-I-IacZ fusion, Chinese Journal of Agricultural Biotechnology, 7(4), 1999, 358-362.
[25] Cheng Ping, Wu L, Yu Ziniu, and Arthur I. Aronson, Subspecies-dependent regulation of Bacillus thuringiensis protoxin genes, Applied and Environmental Microbiology, 65(5), 1999, 1849-1853.
[26] Cheng Ping, Arthur I. Aronson, and Yu Ziniu, Study about Bacillus thuringiensis deletional upstream of cry1A promoter, Biotechnology, 10(4), 2000, 3-8.
[27] Zhang Hongyu, Zeng Xiaohui, Yu Ziniu, and Deng Wangxi, Characterization and toxicity of Bacillus thuringiensis isolates from warehouse to Spodoptera exigua (Lep., Noctuidae), Journal of Applied Entomology, 125, 2001, 211-214.
[28] Zhang Lili, Arthur I. Aronson, and Yu Ziniu, Effects of carbon sources on the expression of cry1D-lacZ in Bacillus thuringiensis, Hereditas,24(3), 2000, 301-304.
[29] T.C.Hodgman, Y.u Ziniu, J. Shen, and D.J. Ellar, Identification of a cryptic gene associated with an insertion sequence not preciously identified in Bacillus thuringiensis, FEMS Microbiology Letters, 114, 1993, 23-29.
[30] Sun Ming, Yu Ziniu, Liu Ziduo, and Li Fudi, Modification of Bacillus thuringiensis insecticidal crystal protein gene cry218, Chinese Journal of Biologinal Control, 12(3), 1996, 127-129.
[31] Wang Qingfeng, and Yu Ziniu, Modification of Bacillus thuringiensis insecticidal crystal protein cry1E, Journal of Huazhong Agricultural University, 17(5), 1998, 465-468.
[32] Shao Zongze, Liu Ziduo, and Yu Ziniu, Effects of the 20-Kilodalton helper protein on Cry1Ac production and spore formation in Bacillus thuringiensis, Applied and Environmental Microbiology 67(12), 2001, 5362-5369.
[33] Shao Zongze, Liu Ziduo, and Yu Ziniu, The Effects of 20KDa protein on the expression and crystallization of Cry1A in Bacillus thuringiensis. Biotechnology of Bacillus thuringiensis (Eds. Yu Ziniu, Sun Ming, and Liu Ziduo), 3, 1999, 122-125. Science Press New York.
[34] Lu Songqing, Liu Ziduo, and Yu Ziniu, Transfer of cry1C gene into Bacillus thuringiensis by electroporation to construct strain with broader insecticidal activity, Journal of Biological Engineering, 16(4), 2000, 56-59.
[35] Yue Chaoyin, Liu Ziduo, Li Lin, Jiang Hao, Wei Fang, and Yu Ziniu, Study of insecticidal crystal protein gene cry3A transformant into Bacillus thuringiensis, Journal of Wuhan University (Natural Science Edition), 44, 1998, 51-52.
[36] Yue Chaoyin, and Yu Ziniu, Studies on transformation of transpositive plasmids into Bacillus thuringiensis, Journal of Huazhong Agricultural University, 20(4), 2001, 363-364.