Rapid Amplification of cDNA Ends (RACE)

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Published: Mar 30, 2018
Keywords:
RACE, PCR

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Liu Peigang
Bioengineering Department of Harbin, Institute of Technology, Harbin, China.
Yang Qian*
Bioengineering Department of Harbin, Institute of Technology, Harbin, China.

Abstract

Rapid amplification of cDNA ends is a polymerase chain reaction-based technique which was developed to facilitate the cloning of full-length cDNA 5’ and 3’ ends after a partial cDNA sequence has been obtained by other methods. While RACE can yield complete sequences of cDNA ends in only a few days, the RACE procedure frequently results in the exclusive amplification of truncated cDNA ends. Many investigators have suggested modifications to the RACE protocols to improve the effect of the technique.


Keywords: RACE, PCR


Corresponding author: E-mail: yangq@hope.hit.edu.cn

Article Details

Issue
Vol. 4 No. 1 (2004)
Section
Review Ariticle

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