Antibacterial Activity of Extracts Derived from the Unicellular Green Algae, Chlorella spp.

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Weena Choochote*
Pongtorn Kruawanishtham
Phansa Phumprasert
Wittawas Janvechsakda

Abstract

Antibacterial substances from five strains of unicellular green algae, Chlorella spp., were extracted in water: methanol : chloroform (1 : 2 : 1, v/v). The highest yield of crude algal extract (0.24 g/g dry weight) was obtained from Chlorella spp. A. 0505. Various concentrations of the extracts were tested against 6 genera of bacteria for antibacterial activity by the disc diffusion method. It was found that the crude algal extracts (4 mg/disc) from Chlorella vulgaris TISTR 8580 strongly inhibited the growth of Staphylococcus aureus and Streptococcus pyogenes. Moreover, the extracts from Chlorella spp. A. 0505 and Chlorella vulgaris TISTR 8261 have been found to effectively inhibit the growth of Pseudomonas aeruginosa and Bacillus subtilis, respectively.


Keywords: Antibacterial activity, unicellular green algae, Chlorella spp.


Corresponding author: E-mail: Keweena@kmitl.ac.th

Article Details

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Original Research Articles

References

[1] Chang, T., S. Ohta, N. Ikegami, H. Miyata, T. Kashimoto and M. Konko. Antibiotic substances produced by a marine green alga, Dunaliella primolecta. Biores. Technol. 44, 1993, 149 – 153.
[2] Vlachos, V., AT. Critchley and AV. Holy. Antimicrobial activity of extracts from selected southern African marine macroalgae. South African J. of science. 93, 1997, 328 – 332.
[3] Pushparaj, B., E. Pelosi and F. Juttner. Toxicological analysis of the marine cyanobacterium Nodularia harveyana. J. appl. Phycol. 10, 1999, 527 – 530.
[4] Anggadiredja, J., LB. Astuti, W. Sujatmiko and H. Purwoto. Antifungal activity of Laurencia sp. : a preliminary study. In : Proceedings of the 2nd Asia-Pacific marine biotechnology conference and 3rd Asia-Pacific conference on algal biotechnology. 7 – 10 May 1997 Phuket Thailand. 1997, 249 - 254.
[5] Patterson, GML., KK. Baker, CL. Baldwin, CM. Bolis, FR. Capland, LK. Larsen, IA. Levine, RE. Moore, CS. Nelson, KD. Tschappat, GD. Tuang, MR. Boyd, JH. Caedellina, RP. Collins, KR. Gustafson, KM. Snader, OS. Weislow and RA. Lewin. 1993 Antiviral activity of cultured blue-green algae (Cyanophyta). J. Phycol. 29 : 125-130.
[6] Schlegel, I., NT. Done, ND. Chazal and GD. Smith. Antibiotic activity of new cyanobacterial isolates from Australia and Asia against green algae and cyanobacteria. J. appl. Phycol. 10, 1999, 417-479.
[7] Matsubara, K., Y. Matsuura, K. Hori and K. Miyazawa. An anticoagulant proteoglycan from the marine green alga, Codium pugniformis. J. appl. Phycol. 12, 2000, 9 – 14.
[8] Pratt, R., TC. Dniels, JJ. Eiler, JB. Gunnisor, WD. Kumler, JF. Oneto and LA. Strait. Chlorellin, an antibacterial substance from Chlorella. Science. 99, 1994, 351 – 352.
[9] Shamsudin, L. Useful Biochemical components of selected Malaysian brown seaweeds. In : Proceedings of the 2nd Asia-Pacific marine biotechnology conference and 3rd Asia-Pacific conference on algal biotechnology. 7-10 May 1997 Phuket Thailand. 1997, 235-242.
[10] Qingxiang, L., Y. Xiaojun, F. Xiao and H. Lijun. Studies on extraction procedure of phlorotannins. In : Proceedings of the 2nd Asia-Pacific marine biotechnology conference and 3rd Asia-Pacific conference on algal biotechnology. 7-10 May 1997 Phuket. Thailand. 1997, 231-233.
[11] Kodani, S., K. Ishida and M. Murakami. Dehydroradiosumin, a trypsin inhibitor from the cyanobacterium Anabaena cylindrica J. Nat. Prod. 61, 1998, 6(854-856).
[12] Attasampunna, P. TISTR culture collection fifth edition Bangkok MIRCEN. Thailand Institute of Scientific and Technological Research Bangkok, Thailand, 1995.
[13] Robles Centeno, PO., DL. Ballantine and WH. Gerwick. Dyanmics of antibacterial activity in three species of Caribbean marine algae as a function of habitat and life history. In: Robles Centeno, PO. And DL. Ballantine. 1999. Effects of culture conditions on production of antibiotically active metabolites by the marine alga Spyridia filamentosa (Ceramiaceae, Rhodophyta). I. Light. J. appl. Phycol. 10, 1996, 453-460.