Mating System of the Edible Mushroom, Pleurotus cystidiosus
Article Sidebar
Main Article Content
Abstract
Mating system or sexual system determines sexual compatibility in fungi. The aim of this research was to determine the type of mating system of the dibble commercial mushroom, Pleurotus cystidiosus. The experiments were carried out by crossing each pair of the 14 single spore isolates (SSIs, monokaryons) from one single fruiting body of P. cystidiosus in all combinations, on MEA plates, and incubated at 30°C for 3 weeks, followed by clamp connection examinations. The presence or absence of clamps indicates compatible or incompatible mating, respectively. The ratio number of compatible matings to the number of total mating of P. cystidiosus was determined to be 1:4. This ratio indicates that the sexuality of the species is an example of tetrapolar (bifactorial) heterothallism. The 14 SSIs were also separated into 4 groups according to the four mating types (A1B1, A1B2, A2B1, and A2B2) of the species.
Keywords: mating system, mating type, incompatibility, tetrapolar, Pleurotus spp.
Corresponding author: E-mail: kdpannee@kmitl.ac.th
Article Details
Copyright Transfer Statement
The copyright of this article is transferred to Current Applied Science and Technology journal with effect if and when the article is accepted for publication. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this contribution is original and that he/she has full power to make this grant. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors.
Here is the link for download: Copyright transfer form.pdf
References
[2] J.R. Raper, Genetics of Sexuality in Higher Fungi. Ronald Press Company, New York, 1966.
[3] K.A. Bartholomen, A.L. Marion, C.P. Novotny, and R.C. Ullrich, A Case Study of Fungal Development and Genetics: Schizophyllum commune. In C.J. Bos (editor: Fungal Genetics, Principles and Practices, New York: Marcel Dekker, Inc., 1996.)
[4] R.H. Peterson, and C.S. Ridley, A New Zealand Pleurotus with Multiple-Species Sexual Compatibility, Mycologia, 88(2), 1996, 198-207.
[5] R.T. Moore, Mating Type Factors in Pleurotus cystidiosus, Trans Br. Mycol. Soc., 85(2), 1985, 345-357.
[6] R.H. Petersen, and I. Krisai-greihuber, Type Specimen Studies in Pleurotus, Persoonia, 17, 1999, 1-20.
[7] J.E. Roxon, and S.C. Jong, Sexuality of the Edible Mushroom Pleurotus sajor-caju, Mycologia, 69, 1977, 203-205.
[8] O.S. Isikhuemhen, J. Moncalvo, F. Nerud, and R. Vigalys, Mating Compatibility and Phylogeography in Pleurotus tuberregium, Myc. Res., 104(6), 2000, 732-737.