Determination of Hepatitis B Surface Antigen Particles by Elisa

Main Article Content

U.N. Ekwenye*

Abstract

Hepatitis B surface Antigen (HBsAg) expressed in Pichia pastoris assemble into particulate structures. Enzyme-linked immunosorbent assay (ELISA) was used to determine these particles. The covalent attachment of enzymes to antibody molecules created an immunological tool possessing both high specificity and high sensitivity. The specificity, rapidity and inexpensive nature of ELISAs have insured their prominence in clinical and veterinary medicine.


Keywords: Hepatits B, Pichia pastoris, ELISA, clinical and veterinary medicine, Antigen


Corresponding author: E-mail:  ekwenyeuchechi@yahoo.com

Article Details

Section
Short Communications

References

[1] www.nfid.org/factheets/hephadult.html
[2] Boitieux, J.L., Desmet, G. and Thomas, D. 1978 Potentiometric Determination of Hepatitis B Surface Antigen in Biological Fluids, Clinical Chim Acta, 88(2) 329-336.
[3] Frosner, G.G., Schmcrus, H., Wieldmann, K.H., Zachoval, R., Bayerl, B., Backer, U., Gathof, G.A. and Sugg, U. 1982 Diagnostic Significance of Quantitative Determination of Hepatitis B Surface Antigen in Acute and Chronic Hepatitis Infection, European Journal of Clinical Microbiology, 1(1) 52-58.
[4] Chiaramonete, M. Heathcote, J. and Sherlock, S. 1977 Detection by Three Techniques of Hepatisis B Surface Antigen (HBsAg) and Determination of HBsAg and Anti-HBs Titres in Patients with Chronic Liver Disease, Gut, 18, 1-6.
[5] Higgins, D.R and Cregg, J.M. 1998 Pichia Protocols Humana Press. New Jersey, p. 268.
[6] Hermann, G., Rita, S. and Ridder, R. 1998 Secretion of SCFV Antibody fragments. In Pichia protocols edited by Higgins, D.R. and Cregg, J.M. Humana Press Inc. New Jersey, pp. 179-192.
[7] Vassileva, A., Chugh, D.A., Swaminathan, S. and Khanna, N. 2001 Effect of Copy Number on the Expression Levels of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia pastoris, Protein Expression and Purification, 21, 71-80.
[8] Cregg, J.M., Tschopp, S.F., Stillan, C.S.F., Stillman, C., Siegel, R., Akong, M., Craig, W.S., Buckholz, R.G, Madden, K.R., Kelaris, P.A., Davis, G.R, Smdey, B.L., Cruze J., Torregrososa, R., Velicelibi, G and Thill, G.P 1987 High Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia pastoris Biotechnology, 5, 479-495.
[9] Himan, B. Chugh, Swaminathan, S and Khana, N. 2001 Expression and Purification of Dengue Virus types 2 Envelope Protein as a Fusion with Hepatitis B Surface Antigen in Pichia pastors, Protein Expression and purification, 23, 84-96.