Resistance to Quinone-outside Inhibitor (QoI) Fungicides in Colletotrichum Species Isolated from Anthracnose Disease Occurring in Thailand
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Abstract
Colletotrichum species are plant pathogens causing anthracnose diseases in many crops that result in significant reduction of quality and quantity of the crop products. Management of these diseases has become increasingly difficult recently due to the development of fungicide resistance in pathogens. Thus, monitoring for fungicide sensitivity in the pathogen is important to manage these diseases. The isolates of Colletotrichum species were isolated from anthracnose disease on various crops collected during 2016-2017 in central and southern Thailand. They were tested for their mycelium growth sensitivity to azoxystrobin, a quinone-outside inhibitor (QoI) fungicide and their internal transcribed spacer (ITS) and 5.8S regions of rDNA were analyzed. The results showed that eleven out of twenty four isolates of Colletotrichum species were resistant to azoxystrobin. They were able to grow on PDA amended with 100 mg/l azoxystrobin in the presence of salicylhydroxamic acid (SHAM) at 100 mg/l. Moreover, two isolates of azoxystrobin-resistant Colletotrichum spp. produced much larger lesions than azoxystrobin-sensitive isolates on mango fruits after treatment with 100 mg/l azoxystrobin. This is the first report on the occurrence of azoxystrobin resistance in Colletotrichum species present in Thailand and will contribute to the management of these important diseases in the future.
Keywords: anthracnose; azoxystrobin; Colletotrichum; fungicide resistance; mango; QoIs
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References
[2] Prihastuti, H., Cai, L., Chen, H., McKenzie, E.H.C. and Hyde, K.D., 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity, 39, 89-109.
[3] Kuenpech, W. and Akarapisan, A., 2014. Biological control of anthracnose disease in Lady’s Slipper using Bacillus subtilis isolate B6. Journal of Agricultural Technology, 10, 449-457.
[4] Photita, W., Taylor, P.W.J., Ford, R., Hyde, K.D. and Lumyong, S., 2005. Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Diversity, 18, 117-133.
[5] Chantrasri, P., 2019. Control of Anthracnose Disease in Mango Orchard. Science and Technology Research Institute. [online] Available at: https://stri.cmu.ac.th/article_ detail.php?id=11.
[6] Plant Protection Group, Nakhon Si Thammarat Provincial Agricultural Extension Office, 2013. Dried Chili Disease or Anthracnose. News of forecasting epidemics and pests. [online] Available at: http://www.nakhonsri.doae.go.th/web/files/antrac.pdf.
[7] Fungicide Resistance Action Committee, 2018. FRAC Code List ©*2018: Fungicides sorted by mode of action. [online] Available at: http://www.phi-base.org/images/fracCodeList.pdf.
[8] Kongtragoul, P., Nalumpang, S., Miyamoto. Y., Izumi, Y. and Akimitsu, K., 2011. Mutation at codon 198 of Tub2 gene for carbendazim resistance in Colletotrichum gloeosporioides causing mango anthracnose in Thailand. Journal of Plant Protection Research, 51, 377-384.
[9] Nalumpang, S., Miyamoto, Y., Miyake, C., Izumi, Y., Akitmitsu, K. and Kongtragoul, P., 2010. Point mutations in the beta-tubulin gene conferred carbendazim-resistant phenotypes of Colletotrichum gloeosporioides causing ‘Nam Dok Mai’ mango anthracnose. International Journal of Agricultural Technology, 6, 365-378.
[10] Fernández-Ortuño, D., Torés, J.A., de Vicente, A. and Pérez-García, A., 2008. Mechanisms of resistance to QoI fungicides in phytopathogenic fungi. International Microbiology, 11, 1-9.
[11] Gisi, U., Sierotzki, H., Cook, A. and McCaffery, A., 2002. Mechanisms influencing the evolution of resistance to Qo inhibitor fungicides. Pest Management Science, 58, 859-867.
[12] Sierotzki, H., Wullschleger, J. and Gisi, U., 2000. Point mutation in cytochrome b gene conferring resistance to strobilurin fungicides in Erysiphe graminis f sp. tritici field isolates. Pesticide Biochemistry and Physiology, 68, 107-112.
[13] Office of Agricultural Economics, 2018. Quantity and value of imports of agricultural hazardous substances in 2011-2017. [online] Available at: http://oldweb.oae.go.th/ economicdata/pesticides.html.
[14] Avila-Adame, C., Olaya, G. and Köller, W., 2003. Characterization of Colletotrichum graminicola isolates resistant to strobilurin-related QoI fungicides. Plant Disease, 87, 1426-1432.
[15] Inada, M., Ishii, H., Chung, W.H., Yamada, T., Yamaguchi, J. and Furuta, A., 2008. Occurrence of strobilurin resistant strains of Colletotrichum gloeosporioides (Glomerella cingulata), the causal fungus of strawberry anthracnose. Japanese Journal of Phytopathology, 74, 114-117.
[16] Wong, F.P., Midland, S.L. and Karla, A.C., 2007. Occurrence and distribution of QoI-resistant isolates of Colletotrichum cereale from annual bluegrass in California. Plant Disease, 91, 1536-1546.
[17] Young, J.R., Tomaso-Peterson, M, Tredway, L.P. and Cerda, K., 2010. Occurrence and molecular identification of azoxystrobin resistant Colletotrichum cereale isolates from golf course putting greens in the southern United States. Plant Disease, 94, 751-757.
[18] Hu, M.J., Grabke, A., Dowling, M.E., Holstein, H.J. and Schnabel, G., 2015. Resistance in Colletotrichum siamense from peach and blueberry to thiophanate-methyl and azoxystrobin. Plant Disease, 99, 806-814.
[19] Forcelini, B.B., Seijo, T.E., Amiri, A. and Peres, N.A., 2016. Resistance in strawberry isolates of Colletotrichum acutatum from Florida to quinone-outside inhibitor fungicides. Plant Disease, 100, 2050-2056.
[20] Piccirillo, G., Carrieria, R., Polizzib, G., Azzaroc, A., Lahoza, E., Fernández-Ortuñod, D. and Vitaleb, A., 2018. In vitro and in vivo activity of QoI fungicides against Colletotrichum gloeosporioides causing fruit anthracnose in Citrus sinensis. Scientia Horticulturae, 236, 90-95.
[21] Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I., Martin-Mex, R., Nexticapan-Garcez, A. and Perez-Brito, D., 2015. Sensitivity of Colletotrichum truncatum to four fungicides and molecular characterization of thiabendazole-resistant isolates. Plant Disease, 99, 1590-1595.
[22] Kim, S., Min, J., Back, D., Kim, H., Lee, S. and Kim, K., 2016. Assessment of QoI resistance in Colletotrichum spp. isolated from boxthorn and apple in Korea. Phytopathology, 106 (Suppl. 12), 71.
[23] Saitoh, K., Togashi, K., Arie, T. and Teraoka, T., 2006. A simple method for a mini-preparation of fungal DNA. Journal of General Plant Pathology, 72, 348-350.
[24] Ishii, H., Zhen, F., Hu, M., Li, X. and Schnabel, G., 2016. Efficacy of SDHI fungicides, including benzovindiflupyr, against Colletotrichum species. Pest Management Science, 72, 1844-1853.
[25] White, T.J., Bruns, T., Lee, S. and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J. eds. PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, pp. 315-322.
[26] Mongkolporn, O. and Taylor, P.W.J., 2018. Chili anthracnose: Colletotrichum taxonomy and pathogenicity. Plant Pathology, 67, 1255-1263.
[27] De Silva, D.D., Ades, P.K., Crous, P.W. and Taylor, P.W.J., 2016. Colletotrichum species associated with chili anthracnose in Australia. Plant Pathology, 66, 254-267.
[28] Noor, N.M. and Zakaria, L., 2018. Identification and characterization of Colletotrichum spp. associated with chili anthracnose in peninsular Malaysia. European Journal Plant Pathology, 151, 961-973.
[29] Phoulivong, S., Cai, L., Chen, H., McKenzie, H.E.H.C., Abdelsalam, K., Chukeatirote, E. and Hyde, K. D., 2010. Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Diversity, 44, 33-43.
[30] Sharma, G., Gryzenhout, M., Hyde, K.D., Pinnaka, A.K. and Shenoy, B.D., 2015. First report of Colletotrichum asianum causing mango anthracnose in South Africa. Plant Disease, 99, 725.
[31] de Souza, A., Delphino Carboni, R.C., Wickert, E., de Macedo Lemos, E.G. and de Goes, A., 2013. Lack of host specificity of Colletotrichum spp. isolates associated with anthracnose symptoms on mango in Brazil. Plant Pathology, 62, 1038-1047.
[32] Rakesh, P.K. and Singh, R., 2017. Anthracnose of mango incited by Colletotrichum gloeosporioides: A comprehensive review. International Journal of Pure & Applied Bioscience, 5, 48-56.
[33] Krishnapillai, N. and Wilson Wijeratnam, R.S., 2014. First Report of Colletotrichum asianum causing anthracnose on Willard mangoes in Sri Lanka. New Disease Reports, 29, 1.
[34] Mo, J., Zhao, G., Li, Q., Solangi, G.S., Tang, L., Guo, Tangxun and Huang, S., 2018. Identification and characterization of Colletotrichum species associated with mango anthracnose in Guangxi, China. Plant Disease, 102, 1283-1289.
[35] Lima, N.B., Batista, M.V.D.A., De Morais Jr, M.A., Barbosa, M.A.G., Michereff, S.J., Hyde, K.D. and Câmara, M.P.S., 2013. Five Colletotrichum species are responsible for mango anthracnose in northeastern Brazil. Fungal Diversity, 61, 75-88.
[36] Cannon, P.F., Damm, U., Johnston, P.R. and Weir, B.S., 2012. Colletotrichum - current status and future directions. Studies in Mycology, 73, 181-213.
[37] He, L., Li, X., Gao, Y., Li, B., Mu, W. and Liu, F., 2019. Characterization and fungicide sensitivity of Colletotrichum spp. from different hosts in Shandong, China. Plant Disease, 103, 34-43.