Physiological and Metabolic Modifications in Response to Nanocarbon in Callus of Indica Rice Cultivar

Article Sidebar

PDF
Published: Oct 2, 2019
Keywords:
callus; growth; metabolite; nanocarbon; rice

Main Article Content

Thanawat Sutjaritvorakul
Faculty of Science and Technology, Pathumwan Institute of Technology, Bangkok, Thailand
Sutee Chutipaijit*
College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand

Abstract

Nanocarbon has been shown to be implicated in the response of plants to an assortment of positive or negative impacts. In the present research, the effects of nanocarbon on cell growth and secondary metabolite production on rice callus were studied. Different concentrations of nanocarbon (0-1000 mg l−1) were added into callus induction media to investigate the effects on the growth of callus, and secondary metabolite production in indica rice callus (Oryza sativa L. cv. Pathumthani1). The growth of callus was measured in terms of the size and weight of callus, and secondary metabolite production was determined based on the levels of total phenolic compounds and flavonoid production. The results showed that the callus induction, the callus growth and the secondary metabolite production in rice callus after treatment with nanocarbon were significantly different when compared to control (without treatment with nanocarbon). The rice callus treated with low (100 and 200 mg l-1) and moderate (400 and 600 mg l-1) level of nanocarbon displayed an enhancement in callus growth, total phenolic compounds and flavonoid production. However, the induction of callus, cell growth, and secondary metabolite production were decreased when rice callus was treated with high concentrations of nanocarbon (800 and 1000 mg l-1). The results of this research showed that the nanocarbon application of 400-600 mg l-1 had potential to induce the cell growth and secondary metabolite production. This research suggests further investigation in using different concentrations of nanocarbon on other plant species for potential pharmaceutical application.
Keywords: callus; growth; metabolite; nanocarbon; rice

*Corresponding author: Tel.: +668-6667-7036 Fax: +662-329-8265


                                           E-mail: natadee24@hotmail.com

Article Details

Issue
Vol. 20 No. 1 (2020)
Section
Original Research Articles

Copyright Transfer Statement

 The copyright of this article is transferred to Current Applied Science and Technology journal with effect if and when the article is accepted for publication. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, electronic form (offline, online) or any other reproductions of similar nature.

 The author warrants that this contribution is original and that he/she has full power to make this grant. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors.

Here is the link for download: Copyright transfer form.pdf

 

 

References

Mandal, C., Bera, S., Dey, N. and Adak, M., 2016. Physiological alterations of Salvinia natans L. exposed to aluminium stress and its interaction with polyamine. Plant Science Today, 3, 195-206.

Wang, W., Yang, C., Tang, X., Gu, X., Zhu, Q., Pan, K. and Ma, D., 2014. Effects of high ammonium level on biomass accumulation of common duckweed Lemna minor L. Environmental Science and Pollution Research, 21, 14202-14210.

Ardebili, N.O., Saadatmand, S., Niknam, V. and Khavari-Nejad, R.A., 2014. The alleviating effects of selenium and salicylic acid in salinity exposed soybean. Acta Physiologiae Plantarum, 36, 3199-3205.

Habibi, G., 2013. Effect of drought stress and selenium spraying on photosynthesis and antioxidant activity of spring barley. Acta agriculturae Slovenica, 101, 31-39.

Ozkan, G., Sagdic, O., Gokturk, R.S., Unal, O. and Albayrak, S., 2010. Study on chemical composition and biological activities of essential oil and extract from Salvia pisidica. LWT-Food Science and Technology, 43, 186-190.

Shaw, A.K., Ghosh, S., Kalaji, H.M., Bosa, K., Brestic, M., Zivcak, M. and Hossain, Z., 2014. Nano-CuO stress induced modulation of antioxidative defense and photosynthetic performance of Syrian barley (Hordeum vulgare L.). Environmental and Experimental Botany, 102, 37-47.

Asgari-Targhi, G., Iranbakhsh, A. and Ardebili, Z.O., 2018. Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micropropagation of Capsicum annuum. Plant Physiology and Biochemistry, 127, 393-402.

Gogos, A., Knauer, K. and Bucheli, T.D., 2012. Nanomaterials in plant protection and fertilization, current state, foreseen applications, and research priorities. Journal of Agricultural and Food Chemistry, 60, 9781-9792.

Pandey, A.C., Sanjay, S.S. and Yadav, R.S., 2010. Application of ZnO nanoparticles in influencing the growth rate of Cicer arietinum. Journal of Experimental Nanoscience, 5, 488-497.

Dimkpa, C.O., Latta, D.E., McLean, J.E., Britt, D.W., Boyanov, M.I. and Anderson, A.J., 2013. Fate of CuO and ZnO nano and micro particles in the plant environment. Environmental Science and Technology, 47, 4734-4742.

Adhikari, T., Kundu, S., Biswas, A.K., Tarafdar, J.C. and Rao, A.S., 2012. Effect of copper oxide nano particle on seed germination of selected crops. Journal of Agricultural Science and Technology, 2, 815-823.

Rahmani, F., Peymani, A., Daneshvand, E. and Biparva, P., 2016. Impact of zinc oxide and copper oxide nano-particles on physiological and molecular processes in Brassica napus. Indian Journal of Plant Physiology, 21, 122-128.

Zaytseva, O. and Neumann, G., 2016. Carbon nanomaterials: production, impact on plant development, agricultural and environmental applications. Chemical and Biological Technologies in Agriculture, 3, 17.

Peng, Q., Dearden, A.K., Crean, J., Han, L., Liu, S., Wen, X. and De, S., 2014. New materials graphyne, graphdiyne, graphone, and graphane: review of properties, synthesis, and application in nanotechnology. Nanotechnology, Science and Applications, 7, 1-29.

Chutipaijit, S. and Sutjaritvorakul, T., 2018. Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (Oryza sativa L.). Chemical Speciation and Bioavailability, 30, 1-8.

Ata-Ul-Karim, S.T., Liu, X.J., Lu, Z.Z., Yuan, Z.F., Zhu,Y. and Cao, W.X., 2016. In-season estimation of rice grain yield using critical nitrogen dilution curve. Field Crops Research, 195, 1-8.

Adams, J., Wright, M., Wagner, H., Valiente, J., Britt, D. and Anderson, A., 2017. Cu from dissolution of CuO nanoparticles signals changes in root morphology. Plant Physiology and Biochemistry, 110, 108-117.

Yang, Z., Chen, J., Dou, R., Gao, X., Mao, C. and Wang, L., 2015. Assessment of the phytotoxicity of metal oxide nanoparticles on two crop plants, maize (Zea mays L.) and rice (Oryza sativa L.). International Journal of Environmental Research and Public Health, 12, 15100-15109

Li, L., Qu, R., De Kochko, A., Frauquet, C. and Beachy, R.N., 1993. An improved rice transformation method using the biolistic method. Plant Cell Reports, 12, 250-255.

Hoffmann, A. and Poorter, H., 2002. Avoiding bias in calculations of relative growth rate. Annals of Botany, 80, 37-42.

Alothman, M., Bhat, R. and Karim, A.A., 2009. Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chemistry, 115, 785-788.

Pedro, A.C., Granato, D. and Rosso, N.D., 2016. Extraction of anthocyanins and polyphenols from black rice (Oryza sativa L.) by modeling and assessing their reversibility and stability. Food Chemistry, 191, 12-20.

Brand-Williams, W., Cuvelier, M.E. and Berset. C., 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25-30.

Ivanova, K., Dimitrova, V., Georgieva, T. and Markovska, Y., 2015. Effect of soil salinity on growth, gas exchange and antioxidant defence of two Paulownia lines. Genetics and Plant Physiology, 4, 163-173

Gupta, S.D. and Jatothu, B., 2013. Fundamentals and applications of light emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechnology Reports, 7, 211-220.

Samart, S. and Chutipaijit, S., 2018. Modifications of morphological and physiological characteristics of pigmented-rice seedlings by application of titanium dioxide nanoparticles. AIP Conference Proceedings, 2010, 020003.

Wang, X., Han, H., Liu, X., Gu, X., Chen, K. and Lu, D., 2012. Multi-walled carbon nanotubes can enhance root elongation of wheat (Triticum aestivum) plants. Journal of Nanoparticle Research, 14, 1-10.

Tiwari, D.K., Dasgupta-Schubert, N., Villasenor Cendejas, L.M., Villegas, J., Carreto Montoya, L. and Borjas Garcia, S.E., 2014. Interfacing carbon nanotubes (CNT) with plants: enhancement of growth, water and ionic nutrient uptake in maize (Zea mays) and implications for nanoagriculture. Applied Nanoscience, 4, 577-591.

Husen, A. and Siddiqi, K.S., 2014. Carbon and fullerene nanomaterials in plant system. Journal of Nanotechnology, 12, 1-10.

Khodakovskaya, M., Dervishi, E., Mahmood, M., Xu, Y., Li, Z., Watanabe, F. and Biris, A.S., 2009. Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano, 3, 3221-3227.

Villagarcia, H., Dervishi, E., de Silva, K., Biris, A.S. and Khodakovskaya, M.V., 2012. Surface chemistry of carbon nanotubes impacts the growth and expression of water channel protein in tomato plants. Small, 8, 2328-2334.

Canas, J.E., Long, M., Nations, S., Vadan, R., Dai, L., Luo, M., Ambikapathi, R., Lee, E.H. and Olszyk, D., 2008. Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species. Environmental Toxicology and Chemistry, 27, 1922-1931.

Flores, D., Chacón, R., Alvarado, L., Schmidt, A., Alvarado, C. and Chaves, J., 2014. Effect of using two different types of carbon nanotubes for blackberry (Rubus adenotrichos) in vitro plant rooting, growth and histology. American Journal of Plant Sciences, 5, 3510-3518.

Khodakovskaya, M.V., Kim, B.S., Kim, J.N., Alimohammadi, M., Dervishi, E., Mustafa, T. and Cernigla, C.E., 2013. Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Small, 9, 115-123.

Kumari, A., Baskaran, P. and Van Staden, J., 2016. In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant. Plant Cell, Tissue and Organ Culture, 124, 97-104.

Liao, D.Y., Chai, Y.C., Wang, S.H., Chen, C.W. and Tsai, M.S., 2015. Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects. Journal of Food and Drug Analysis, 23, 294-302.

Mahboubi, A., Kamalinejad, M., Ayatollahi, A.M. and Babaeian, M., 2014. Total phenolic content and antibacterial activity of five plants of Labiatae against four foodborne and some other bacteria. Iranian Journal of Pharmaceutical Research, 3, 559-566.

Sergeant, K., Kieffer, P., Dommes, J., Hausman, J.F. and Renaut, J., 2014. Proteomic changes in leaves of poplar exposed to both cadmium and low-temperature. Environmental and Experimental Botany, 106, 112-123.

Song, S.Y., Chen, Y., Chen, J., Dai, X.Y. and Zhang, W.H., 2011. Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress. Planta, 234, 331-345.

Chutipaijit, S., 2016. Changes in physiological and antioxidant activity of indica rice seedlings in response to mannitol-induced osmotic stress. Chilean Journal of Agricultural Research, 76, 455-462.

Ghorbanpour, M., 2015. Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide. Indian Journal of Plant Physiology, 20, 249-256.

Aghajani, Z., Pourmeidani, A. and Ekhtiyari, R., 2013. Effect of nano-silver on stages of plant growth and yield and composition of essential of Thymus kotchyanus Boiss. & Hohen. African Journal of Agricultural Research, 8, 707-710.

Hatami, M., Ghorbanpour, M. and Salehiarjomand, H., 2014. Nanoanatase TiO2 modulates the germination behavior and seedling vigority of the five commercially important medicinal and aromatic plants. Journal of Biological and Environmental Sciences, 8, 53-59.

Ghorbanpour, M. and Hatami, M., 2015. Changes in growth, antioxidant defense system and major essential oils constituents of Pelargonium graveolens plant exposed to nano-scale silver and thidiazuron. Indian Journal of Plant Physiology, 20, 116-123.

Yang, H., Liu, C., Yang, D., Zhang, H. and Xi, Z., 2009. Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: the role of particle size, shape and composition. Journal of Applied Toxicology, 29, 69-78.

Remya, N., Saino, H.V., Baiju, G.N., Maekawa, T., Yoshida, Y. and Sakthi, K.D., 2010. Nanoparticulate material delivery to plants. Plant Science, 179, 154-163.

Oloumi, H., Soltaninejad, R. and Baghizadeh, A., 2015. The comparative effects of nano and bulk size particles of CuO and ZnO on glycyrrhizin and phenolic compounds contents in Glycyrrhiza glabra L. seedlings. Indian Journal of Plant Physiology, 20, 157-161.

Mohammadia, R., Maali-Amiria, R. and Mantri, N.L., 2014. Effect of TiO2 nanoparticles on oxidative damage and antioxidant defense systems in chickpea seedlings during cold stress. Russian Journal of Plant Physiology, 61, 768-775.

Nagarajan, A., Arivalagan, U. and Rajagurua, P., 2011. In vitro root induction and studies on antibacterial activity of root extract of Costusigneus on clinically important human pathogens. Journal of Microbiology and Biotechnology Research, 1, 67-76.

El-Bakry, A.A., Mostafa, H.A.M. and Alam Eman, A., 2014. Antioxidant and antibacterial activity of callus and adventitious root extracts from Rumex vesicarius L. Journal of Medicinal Plants Research, 8, 479-488.

Baskaran, P., Kumari, A., Naidoo, D. and Van Staden, J., 2015. In vitro propagation and biochemical changes in Aloe pruinosa. Industrial Crops and Products, 77, 51-58.