Main Article Content
The natural reagents from Vigna unguiculata subsp. sesquipedalis were studied and applied in
the determination of tetracycline by UV-Vis spectrophotometry. The method was based on a complexation formation between tetracycline and iron (III) derived from natural plant extract in acetate buffer at pH 5 to give a yellow complex with the optimum absorption at 430 nm. Parameters related to the extraction efficiency of the natural reagent and the factors that affected the determination of tetracycline were examined. Under optimum conditions, linearity was obtained over the range of 1.00 - 20.00 mg l-1. The limit of detection (LOD, 3σ) and limit of quantification (LOQ, 10σ), calculated following IUPAC, were 0.65 and 2.15 mg l-1, respectively. The repeatability and reproducibility for determining 10.00 mg l-1 of tetracycline (n=11) were 3.43%and 5.14%, respectively. The proposed method was successfully applied to the determination of tetracycline in pharmaceutical formulations. The results obtained by the proposed method were in good agreement with the label values verified by the student t-test at the 95% confidence level.
Keywords: Vigna unguiculata subsp. sesquipedalis; tetracycline; UV-Vis spectrophotometer
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 Chopra, I., Hawkey, P.M. and Hinton, M., 1992. Tetracyclines, molecular and clinical aspects. Journal of Antimicrobial Chemotherapy, 29(3), 245-277.
 Katiyar, S.K. and Elend T.D., 1991. Enhanced antiparasitic activity of lipophilic tetracyclines: role of uptake. Antimicrobial Agents and Chemotherapy, 35, 2075-2080.
 Cinquina, A.L., Longo, F., Anastasi, G., Giannetti, L. and Cozzoani, R., 2003. Validation of a high-performance liquid chromatography method for the determination of oxytetracycline, tetracycline, chlortetracycline and doxycycline in bovine milk and muscle. Journal of Chromatography A, 987(1-2), 227-233.
 Arabsorkhi, B. and Sereshti H., 2018. Determination of tetracycline and cefotaxime residues in honey by micro-solid phase extraction based on electrospun nanofibers coupled with HPLC. Microchemical Journal, 140, 241-247.
 Charoenraks, T., Chuanuwatanakul, S., Honda, K., Yamaguchi, Y. and Chailapakul, O., 2005. Analysis of tetracycline antibiotics using HPLC with pulsed amperometric detection. Analytical Sciences, 21(3), 241-247.
 Hsiao, Y.M., Ko, J.L. and Lo, C.C., 2001. Determination of tetracycline and streptomycin in mixed fungicide products by capillary zone electrophoresis. Journal of Agricultural and Food Chemistry, 49(4), 1669-1674.
 Islas, G., Rodriguez, J.A., Perez-Silva, I., Miranda, J.M. and Ibarra, I. S., 2018. Solid-phase extraction and large-volume sample stacking-capillary electrophoresis for determination of tetracycline residues in milk. Journal of Analytical Methods in Chemistry, 2018, 1-7.
 Palaharn, S., Charoenraks, T., Wangfuengkanagul, N., Grudpan, K. and Chailapakul, O., 2003. Flow injection analysis of tetracycline in pharmaceutical formulation with pulsed amperometric detection. Analytica Chimica Acta, 49, 191-197.
 Wong, A., Scontri, M., Materon, E.M., Lanza, M.R.V. and Sotomayor, M.D.P.T., 2015.
Development and application of an electrochemical sensor modified with multi-walled carbon nanotubes and graphene oxide for the sensitive and selective detection of tetracycline. Journal of Electroanalytical Chemistry, 757, 250-257.
 Sun, C.Y., Su, R.F., Bie, J.X., Sun, H.J., Qiao, S.G., Ma, X.Y., Sun R. and Zhang, T.H., 2018.
Label-free fluorescent sensor based on aptamer and thiazole orange for the detection of tetracycline. Dyes and Pigments, 149, 867-875.
 Townshend, A., Ruengsitagoon, W., Thongpoon, C. and Liawruangrath, S., 2005. Flow injection chemiluminescence determination of tetracycline. Analytica Chimica, 541, 103-109.
 Karthikeyan, G., Mohanraj, K., Elango, P.K. and Girishkumar, K., 2004. Synthesis, spectroscopic characterization and antibacterial activity of lanthanide-tetracycline complexes. Transition Metal Chemistry, 29(1), 86-90.
 Chen, R.W. and Huang, H.C., 2009. Transformation of tetracyclines mediated by Mn (II) and Cu (II) ions in the presence of oxygen. Environmental Science and Technology, 43, 401-407.
 Liawruangrath, S., Liawruangrath, B., Watanesk, S. and Ruengsitagoon, W., 2006. Flow injection spectrophotometric determination of tetracycline in a pharmaceutical preparation by complexation with aluminium (III). Analytical Science, 22(1), 15-19.
 Mahgoub, S.A., Khairy, M.E. and Kasem, A., 1974. Complex formation of uranyl acetate with tetracycline and its utilization for their microdetermination. Journal of Pharmaceutical Sciences, 63(9), 1451-1455.
 Silva, P. P., De Paula, F.C.S., Guerra, W., Silveira, J. N., Botelho, F.V., Vieira, L.Q., Bortolotto, T., Fischer, F.L., Bussi, G., Terenzi H. and Pereira-Maia, E.C. 2010. Platinum (II) compounds of tetracyclines as potential anticancer agents: cytotoxicity, uptake and interactions with DNA. Journal of the Brazilian Chemical Society, 21(7), 1237-1246.
 Sultan, M.S., Alzamil, Z.I. and Alarfaj, A.N., 1988. Complexometric-spectrophotometric assay of tetracyclines in drug formulations. Talanta, 35(5), 375.
 Palamy, S. and Ruengsitagoon, W., 2017. A novel flow injection spectrophotometric method using plant extracts as green reagent for the determination of doxycycline. Spectrochimica Acta Part A, 171, 200-206.
 Grenier, D., Huot, M. and Mayrand, D., 2000. Iron-chelating activity of tetracyclines and its impact on the susceptibility of actinobacillus actinomycetemcomitans to these antibiotics.
Antimicrobial Agents and Chemotherapy, 44(3), 763-766.
 Settheeworrarit, T., Hartwell, S.K., Lapanatnoppakhun, S., Jakmunee, J., Christian, G.D. and Grudpan, K., 2005. Exploiting guava leaf extract as an alternative natural reagent for flow injection determination of iron. Talanta, 68, 262-267.
 Iriti, M. and Varoni, E., 2017. Pulses, healthy, and sustainable food sources for feeding the planet. International Journal of Molecular Science, 18(2), 1-8.
 Kongjaimun, A., Kaga, A., Tomooka, N., Somta, P., Vaughan, D.A. and Srinives, P., 2012. The genetics of domestication of yardlong bean, Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis. Annals of Botany, 109 (6), 1185-1200.
 Kongjaimun, A., Somta, P., Tomooka, N., Kaga, A., Vaughan, A.D. and Srinives, P., 2013. QTL mapping of pod tenderness and total soluble solid in yardlong bean [Vigna unguiculata (L.) Walp. subsp. unguiculata cv.-gr. sesquipedalis]. Euphytica, 189(2), 217-223.
 Kumar, S., Yadav, S.S, Tripura, P. and Jatav, S.H., 2017. Use of phosphorus for maximization of mungbean (Vigna radiata L.) (Wilszeck) productivity under semi-arid condition of Rajasthan, India. International Journal of Current Microbiology and Applied Sciences, 6(2), 612-617.
 Miller, J.C. and Miller, J.N. 1993. Statistics for Analytical Chemistry, 3rd ed. New York: Ellis Horwood PTR Prentice Hall.