Improvement in Biomass Production of a Microalga Chlorella sp. S2 Using Starch Processing Wastewater
Main Article Content
Abstract
Microalgae are promising resources for high-quality dietary supplements, pharmaceutical and biofuel production. This study attempted to improve the biomass of a microalga by cultivation in starch processing wastewater. A microalga identified as Chlorella sp. S2 by morphological criterion was isolated from the facultative pond of a noodle making plant. It was able to grow in the starch processing wastewater without addition of nutrients. To increase the biomass productivity of Chlorella sp. S2, inorganic nitrogen sources (NaNO3, NH4Cl and KNO3) were added into the starch processing wastewater. The optimum nitrogen source was potassium nitrate at 7.5 mM of nitrogen, which increased the number of Chlorella sp. S2 up to 1.41´107 cells/ml and the specific growth rate was 0.351 d-1. Under sunlight, the microalga Chlorella sp. S2 also produced high biomass concentration (2.23±0.04 g/l). It means that microalgal cultivation using starch processing wastewater is a great process to produce biomass. In addition, it is able to reduce organic carbon in the wastewater and reduce cost.
Keywords: microalgae; Chlorella; cultivation; starch processing wastewater; biomass production
Corresponding author: Tel.: (+66) 866395551
E-mail: siripornyos@hotmail.com
Article Details
Copyright Transfer Statement
The copyright of this article is transferred to Current Applied Science and Technology journal with effect if and when the article is accepted for publication. The copyright transfer covers the exclusive right to reproduce and distribute the article, including reprints, translations, photographic reproductions, electronic form (offline, online) or any other reproductions of similar nature.
The author warrants that this contribution is original and that he/she has full power to make this grant. The author signs for and accepts responsibility for releasing this material on behalf of any and all co-authors.
Here is the link for download: Copyright transfer form.pdf
References
Hodaifa, G., Sanchez, S., Martinez, E. and Orpez, R., 2013. Biomass production of Scenedesmus bliquus from mixtures of urban and olive-oil mill wastewaters used as culture medium. Applied Energy, 104, 345-352.
Lam, M.K. and Lee, K.T., 2013. Effect of carbon source towards the growth of Chlorella vulgaris for CO2 bio-mitigation and biodiesel production. International Journal of Greenhouse Gas Control, 14, 169-176.
Takeshita, T., Ota, T., Yamazaki, T., Hirata, A., Zachleder, V. and Kawano, S., 2014. Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresource Technology, 158, 127-134.
Li, X., Xu, H. and Wu, Q., 2007. Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors. Biotechnology and Bioengineering, 98, 764-771.
Katiyar, R., Gurjar, B.R., Bharti, R.K., Kumar, A., Biswas, S. and Pruthi, V., 2017. Heterotrophic cultivation of microalgae in photobioreactor using low cost crude glycerol for enhanced biodiesel production. Renewable Energy, 113, 1359-1365.
Ende, S.S.W. and Noke, A., 2019. Heterotrophic microalgae production on food waste and by-products. Journal of Applied Phycology, 31, 1565-1571.
Endo, H., Nakajima, K., Chino, R. and Shirota, M., 2014. Growth characteristics and cellular components of Chlorella regularis, heterotrophic fast growing strain. Agrcultural and Biological Chemistry, 38, 9-18.
Gonzalez, I.E., Parashar, A. and Bressler, D., 2014. Heterotrophic growth and lipid accumulation of Chlorella protothecoides in whey permeate, a dairy by-product stream, for biofuel production. Bioresource Technology, 155, 170-176.
Xie, Z., Lin, W., Liu, J. and Luo, J., 2020. Mixotrophic cultivation of Chlorella for biomass production by using pH-stat culture medium: Glucose-Acetate-Phosphorus (GAP). Bioresource Technology, 313, https://doi.org/10.1016/j.biortech.2020.123506
Mahapatra, D.M., Chanakya, H.N. and Ramachandra, T.V., 2013. Euglena sp. as a suitable source of lipids for potential use as biofuel and sustainable wastewater treatment. Journal of Applied Phycology, 25, 855-865.
Nakanishi, K. and Deuchi, K., 2014. Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris. Journal of Bioscience and Bioengineering, 117, 617-619.
Heredia, A.T., Wei, W., Ruan, R. and Hu, B., 2011. Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass and Bioenergy, 35, 2245-2253.
Yeh, K.L., Chang, J.S. and Chen, W., 2010. Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP-31. Engineering in Life Sciences, 10, 201-208.
Kassas, H.Y. and Mohamed., L.A., 2014. Bioremediation of the textile waste effluent by Chlorella vulgaris. The Egyptian Journal of Aquatic Research, 40, 301-308.
Kumar, P.K., Krishna, S.V., Naidu, S.S., Verma, K., Bhagawan, D. and Himabindu, V., 2019. Biomass production from microalgae Chlorella grown in sewage, kitchen wastewater using industrial CO2 emissions: Comparative study. Carbon Resources Conversion, 2, 126-133.
Ruiz, J., Alvarez, P., Arbib, Z., Garrido, C., Barragan, J. and Perales, J.A., 2011. Effect of nitrogen and phosphorus concentration on their removal kinetic in treated urban wastewater by Chlorella vulgaris. International Journal of Phytoremediation, 13, 884-896.
Chen, C.-Y., Kuo, E.-W., Nagarajan, D., Ho, S.-H., Dong, C.-D., Lee, D.-J. and Chang, J.-S., 2020. Cultivating Chlorella sorokiniana AK-1 with swine wastewater for simultaneous wastewater treatment and algal biomass production. Bioresource Technology, 302, https://doi:10.1016/ j.biortech.2020.122814
Stein, J.R., 1973. Handbook of Phycological Methods: Culture Methods and Growth Measurements. London: Cambridge University Press.
Tansakul, P., Savaddiraksa, Y., Prasertsan, P. and Tongurai, C., 2005. Cultivation of the hydrocarbon-rich alga, Botyococcus braunii in secondary treated effluent from a sea food processing plant. Thai Journal of Agricultural Science, 38, 71-76.
Chaichalerm, S., Pokethitiyook, P., Yuan, W., Meetam, M., Sritong, K., Pugkaew, W., Kungvansaichol, K., Kruatrachue, M. and Damrongphol, P., 2012. Culture of microalgal strains isolated from natural habitats in Thailand in various enriched media. Applied Energy, 89, 296-302.
APHA, AWWA & WPCF, 2005, Standard Methods for the Examination of Water and Wastewater. Washington D.C.: American Public Health Association.
Chen, L., Wang, C., Wang, W. and Wei, J., 2013. Optimal conditions of different flocculation methods for harvesting Scenedesmus sp. cultivated in an open-pond system. Bioresource Technology, 133, 9-15.
Srinuanpan, S., Cheirsilp, B., Kitcha, W. and Prasertsan, P., 2017. Strategies to improve methane content in biogas by cultivation of oleaginous microalgae and the evaluation of fuel properties of the microalgal lipids. Renewable Energy, 113, 1229-1241.
Yeesang, C. and Cheirsilp, B., 2011. Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. Bioresource Technology, 102, 3034-3040.
Shi, X.M., Zhang, X.W. and Chen, F., 2000. Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources. Enzyme and Microbial Technology, 27, 312-318.
Richmond, A. 1986. Handbook of Microalgal Mass Culture. London: CRC Press.
Chu, F.F., Chu, P.N., Shen, X.F., Lam, P.K. and Zeng, R.J., 2014. Effect of phosphorus on biodiesel production from Scenedesmus obliquus under nitrogen-deficiency stress. Bioresource Technology, 152, 241-246.
Xie, M., Qiu, Y., Song, C., Qi, Y., Li, Y. and Kitamura, Y., 2018. Optimization of Chlorella sorokiniana cultivation condition for simultaneous enhanced biomass and lipid production via CO2 fixation. Bioresource Technology Report, 2, 15-20.
Miyamoto, K., 1997. Renewable Biological Systems for Alternative Sustainable Energy Production. Rome: Food and Agricultural Organization of the United Nations.
Shen, Y., Pei, Z., Yuan, W. and Mao, E., 2009. Effect of nitrogen and extraction method on algae lipid yield. International Journal of Agricultureal and Biological Engineering, 2, 51-57.
Converti, A., Casazza, A.A, Ortiz, E.Y., Perego, P. and Borghi, M.D., 2009. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing: Process Intensification, 48, 1146-1151.
Ruangsomboon, S., 2012. Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2. Bioresource Technology, 109, 261-265.