Main Article Content
A rapid, precise, low cost, selective and sensitive paper-based electrochemical device for the determination of H2O2 in milk is described here. Commercially available varnish and a simple hand drawing method were used to develop the hydrophobic pattern to generate a hydrophilic detection zone on the filter paper. The electrode system was fabricated on the detection zone in order to detect H2O2 electrochemically. A commercially available graphite pencil and conductive silver ink were used to fabricate the counter electrode and pseudo-reference electrode, respectively. A paste of Prussian blue (PB) modified graphite, unmodified graphite and phenol-formaldehyde polymer were used to fabricate a PB modified graphite working electrode on paper. This modified electrode showed electrocatalytic activity towards the reduction of H2O2 and it was successfully used for the chronoamperometric detection of H2O2 at 0 V vs Ag reference electrode in 0.1 mol l-1 phosphate buffer, buffered at pH 6.0 in 0.1 mol l-1 KCl. Under optimum conditions, the calibration curve for the H2O2 determination was linear from 5 to 50 mmol l-1 with a detection limit (LoD = + 3SD) of 4.0 mmol l-1. In addition, the PB modified graphite electrode showed selectivity for H2O2 detection in the presence of ascorbic acid, sucrose and citric acid.
Keywords: low-cost device; H2O2 detection; paper-based device; electrochemical device; modified graphite-based electrode
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 Puiu, M., Mirceski, V. and Bala, C., 2021. Paper-based diagnostic platforms and devices. Current Opinion in Electrochemistry, 27, 100726, https://doi.org/10.1016/j.coelec.2021. 100726
 Liana, D.D., Raguse, B., Gooding, J.J. and Chow, E., 2012. Recent advances in paper-based sensors. Sensors, 12(9), 11505-11526.
 Cate, D.M., Adkins, J.A., Mettakoonpitak, J. and Henry, C.S., 2015. Recent developments in paper-based microfluidic devices. Analytical Chemistry, 87(1), 19-41.
 Nery, E.W. and Kubota, L.T., 2013. Sensing approaches on paper-based devices: A review. Analytical and Bioanalytical Chemistry, 405(24), 7573-7595.
 Mahadeva, S.K., Walus, K. and Stoeber, B., 2015. Paper as a platform for sensing applications and other devices: A review. ACS Applied Materials & Interfaces, 7(16), 8345-8362.
 Silva, R.A.B., Montes, R.H.O., Richter, E.M. and Munoz, R.A.A., 2012. Rapid and selective determination of hydrogen peroxide residues in milk by batch injection analysis with amperometric detection. Food Chemistry, 133(1), 200-204.
 Ping, J., Mao, X., Fan, K., Li, D., Ru, S., Wu, J. and Ying, Y., 2010. A Prussian blue-based amperometric sensor for the determination of hydrogen peroxide residues in milk. Ionics, 16(6), 523-527.
 Ping, J., Wu, J., Fan, K. and Ying, Y., 2011. An amperometric sensor based on Prussian blue and poly(o-phenylenediamine) modified glassy carbon electrode for the determination of hydrogen peroxide in beverages. Food Chemistry, 126(4), 2005-2009.
 Siimenson, C., Kruusma, J., Anderson, E., Merisalu, M., Sammelselg, V., Lust, E. and Banks, C.E., 2012. Prussian blue modified solid carbon nanorod whisker paste composite electrodes: Evaluation towards the electroanalytical sensing of H2O2. International Journal of Electrochemistry, 2012(1), https://doi.org/10.1155/2012/238419.
 Moscone, D. and D’ottavi, D., 2001. Construction and analytical characterization of Prussian blue-based carbon paste electrodes and their assembly as oxidase enzyme sensors. Analytical Chemistry, 73(11), 2529-2535.
 Lima, L.S., Rossini, E.L., Pezza, L. and Pezza, H.R., 2020. Bioactive paper platform for detection of hydrogen peroxide in milk. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 227, 117774, https://doi.org/10.1016/j.saa.2019.117774.
 Karyakin, A., 2001. Prussian blue and its analogues: Electrochemistry and analytical applications. Electroanalysis, 13(10), 813-819.
 Karimi, A., Husain, S.W., Hosseini, M., Azar, P.A. and Ganjali, M.R., 2018. Rapid and sensitive detection of hydrogen peroxide in milk by enzyme-free electrochemiluminescence sensor based on a polypyrrole-cerium oxide nanocomposite. Sensors and Actuators B: Chemical, 271, 90-96.
 Gulera, M., Turkoglu, V., Kivrak, A. and Karahan, F. 2018. A novel nonenzymatic hydrogen peroxide amperometric sensor based on Pd@CeO2-NH2 nanocomposites modified glassy carbon electrode. Materials Science and Engineering, 90, 454-460.
 Borràs-Brull, M., Blondeau, P. and Riu, J., 2021. The use of conducting polymers for enhanced electrochemical determination of hydrogen peroxide. Critical Reviews in Analytical Chemistry, 51(3), 204-217.