Mango Seed Kernel Extract as a Natural Antioxidant in Minced Fish During Frozen Storage

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Praphan Pinsirodom*
Chaianun Namngam
Ruchira Taprap
Katherine Gabrielle Thompson


Mango seed kernel extract (MSKE) has been reported to contain high content of phenolic compounds, and exhibit strong in vitro antioxidant activity. This study aimed to evaluate the application of MSKE as natural antioxidant in frozen minced fish products. The effect of MSKE at different concentrations (0%, 1%, 2% and 3% w/w) on retarding lipid and protein oxidation in minced fish samples during frozen storage at -18 ºC was investigated and compared to the chemical antioxidant, BHT (butylated hydroxytoluene) at 0.01% w/w. Physicochemical parameters related to the oxidative stability of minced fish samples including pH, color, peroxide value (PV), thiobarbituric acid reactive substances (TBARS), conjugated diene (CD), protein carbonyl, sulfphydryl groups (SH), and total volatile base nitrogen (TVB-N) were determined. The results showed that the pH values had significantly increased in all samples being treated with MSKE and BHT after 12 weeks of frozen storage. Samples treated with 3% MSKE or BHT were significantly lower in lightness (L*) and redness (a*) but presented no significant effects on yellowness (b*).  Moreover, MSKE at all concentrations studied, and BHT, significantly delayed the increase in values of PV, TBARS, CD, protein carbonyl, and TVB-N and the decrease in values of SH compared to the control sample. At the end of storage, no significant odd odor and flavor assessed by sensory test were detected in frozen minced fish. In conclusion, MSKE at 3% w/w proved to be as efficient as BHT in retarding lipid and protein oxidation in minced fish during frozen storage.

Keywords: mango seed kernel extract; lipid oxidation; protein oxidation; minced fish

*Corresponding author: Tel.: (+66) 23298526





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[1] Ayala-Zavala, J.F., Vega-Vega, V., Rosas-Dominguez, C., Palafox-Carlos, H., Villa Rodriguez J.A., Siddiqui, M.W., Dávila-Aviña, J.E. and González-Aguilar, G.A., 2011. Agro-industrial potential of exotic fruit by- products as a source of food additives. Food Research International, 44, 1866-1874.
[2] Jahurul, M.H.A., Zaidul, I.S.M., Ghafoor, K., Al-Juhaimi, F.Y., Nyam, K.-L., Norulaini, N.A.N., Sahena, F., Omar, A.K.M., 2015. Mango (Mangifera indica L.) by-products and their valuable components: a review. Food Chemistry, 183, 173-180.
[3] Ahmed, A., Saeid, D., Eman, A. and Reham, E., 2007. Egyptian mango by-product 1. Compositional quality of mango seed kernel. Food Chemistry, 103, 1141-52.
[4] Namngam, C. and Pinsirodom, P., 2017. Antioxidant properties selected enzyme inhibition capacities and a cosmetic cream formulation of Thai mango seed kernel extracts. Tropical Journal of Pharmaceutical Research, 16, 9-16.
[5] Namngam, C., Pinsirodom, P. and Boonyuen, S., 2018. Fractionation, antioxidant and inhibitory activity of Thai mango seed kernel extracts. Czech Journal of Food Science, 36, 8-15.
[6] El-Kady, T.M.A., El-Rahman, M.K.A., Toliba, A.O. and El-Maty, S.M.A., 2016. Evaluation of mango seed kernel extract as natural occurring phenolic rich antioxidant compound. Bulletin of the National Nutrition Institute of the Arab Republic of Egypt, 48, 214-243.
[7] Pereirai, A.L.F., Vidal, T.F., Teixeira, M.C., Oliveira, P.F., Pompeu, R.C.F.F., Vieira, M.M.M. and Zapata, J.F.F., 2011. Antioxidant effect of mango seed extract and butylated hydroxytoluene in bologna-type mortadella during storage. Ciência e Tecnologia de Alimentos, Campinas, 31(1), 135-140.
[8] Ahmadi, M., Razavilar, V., Motallebi, A.A., Esmailzadeh Kenari, R. and Khanipour, A.A., 2014. Effects of hydroalcoholic and water extracts of Nettle leaf (Urtica dioica L.) on chemical properties of superchilled minced meat of common Kilka (Clupeonella cultriventris caspia). Journal of Food Quality and Hazards Control, 1, 85-88.
[9] Lourenço, S.C., Moldão-Martins, M. and Alves, V.D., 2019. Antioxidants of natural plant origins: From sources to food industry applications. Molecules, 24, 4132, 3390/molecules24224132.
[10] Viji, P., Venkateshwarlu, G., Ravishankar, C.N. and Gopal, T.K.S., 2017. Role of plant extracts as natural additives in fish and fish products - A review. Fishery Technology, 54, 145-154.
[11] Gai, F., Gasco, L., Ortoff, M., Gonzales-Rodrguez, A. and Parisi, G., 2014. Effect of green tea natural extract on quality parameters and lipid oxidation during storage of tench (Tinca tinca) fillets. Journal of Applied Ichthyology, 30(1), 64-71.
[12] Özen, B.Ö. and Soyer, A., 2018. Effect of plant extracts on lipid and protein oxidation of mackerel (Scomber scombrus) mince during frozen storage. Journal of Food Science and Technology, 55 (1), 120-127.
[13] Bligh, E.G. and Dyer, W.J., 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917.
[14] Grunwald, E.W. and Richards, M.P., 2006. Mechanism of heme protein-mediated lipid oxidation using hemoglobin and myoglobin variants in raw and heated washed muscle. Journal of Agricultural and Food Chemistry, 54(21), 8271-8280.
[15] Maqsood, S., Benjakul, S. and Kamal-Eldin, A., 2012. Haemoglobin-mediated lipid oxidation in the fish muscle: a review. Trends in Food Science and Technology, 28, 33-43.
[16] Frankel, E. N., Huang, S.W., Aeschbach, R. and Prior, E., 1996. Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol and rosmarinic acid, in bulk oil and oil-in-water emulsion. Journal of Agricultural and Food Chemistry, 44, 131-135.
[17] Levine, R.L., Williams, J.A., Stadtman, E.R. and Shacter, E., 1994. Carbonyl assays for determination of oxidatively modified proteins. Method in Enzymology, 233, 346-357.
[18] Ellman, G.L., 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82, 70-77.
[19] Ingridy, S.R., Ligianne, D.S., LiaFerraz, A.S., Lika, A., Pedrp, G.C., Claudio, R.G., Solange, T.C., Marcos, J.M. and Marilia, O., 2014. Shelf life and quality study of minced Tilapia with Nori and Hijiki seaweeds as natural additives. The Science World Journal, 2014, 1-7.
[20] Meilgaard, M., Civille, G.V. and Carr, T.B., 1999. Sensory Evaluation Techniques. 3rd ed. Boca Raton: CRC Press.
[21] Suvanich, V., Jahncke, M.L. and Marshall, D.L. 2000. Changes in selected chemical quality characteristics of channel catfish frame mince during chill and frozen storage. Journal of Food Science, 65(1), 24-29.
[22] Viji, P., Tanuja, S., George, N., Zynudheen, A.A. and Latitha, K.V., 2014. Quality characteristic and shelf life of Sutchi catfish (Pangasianodon hypophthalmus) steaks during refrigerate storage. International Journal of Agricultural Science and Food Technology, 5(2), 105-116.
[23] Tesoriere, L., Butera, D., Gentile, C. and Livrea, M.A., 2007. Bioactive components of caper (Capparis spinosa L.) from Sicily and antioxidant effects in a red meat simulated gastric digestion. Journal of Agricultural and Food Chemistry, 55, 8465-8471.
[24] Zamora, R., Alaiz, M. and Hidalgo, F. J., 1999. Modification of histidine residues by 4, 5)- Epoxy-3-alkenals. Chemical Research in Toxicology, 12, 654-660.
[25] Sriket, P. and La-ongnual, T., 2018. Quality changes and discoloration of Basa (Pangasius bocourti) fillet during frozen storage. Journal of Chemistry, 2018, 2018/5159080.
[26] Sahari, M. A., Nazemroaya, S. and Rezaei. R., 2009. Fatty acid and biochemical changes inmackerel (Scomberomorus commerson) and shark (Carcharhinus dussumieri) fillets during frozen storage. American-Eurasian Journal of Sustainable Agriculture, 3, 519-527.
[27] Tang, S., Kerry, J.P., Sheehan, D., Buckley, D.J. and Morrissey, P.A., 2001. Antioxidative effect of added tea catechins on susceptibility of cooked red meat, poultry and fish patties to lipid oxidation. Food Research International, 34, 651-657.
[28] Banani, D. and Suchandra, C., 2015. Impact of assort spices on lipid quality alteration of refrigerated fish muscle. International Food Research Journal, 22(1), 304-310.
[29] Sarabi, M., Keramat, J. and Kadivar, M., 2017. Antioxidant effect of rosemary extract and BHT on the quality of coated fried Escolar (Lipidocybium avobrunium) fish fillets during frozen storage. Food Research International, 24(2), 525-533.
[30] Lukaszewicz, M., Szopa, J. and Krasowska, A., 2004. Susceptibility of lipids from different flax cultivars to peroxidation and its lowering by added antioxidants. Food Chemistry, 88, 225-231.
[31] Papuc, C., Maria, C., Valentin, N., Costin, P. and Corina, P., 2012. Increase of the stability to oxidation of lipids and proteins in carp muscle (Cyprinus carpio) subject to storage by freezing by polyphenols extracted from sea buckthorn fruits (Hippophae rhamnoides). Revista De Chimie (Bucharest), 63(12), 1198-1203.
[32] Maqsood, S. and Benjakul, S., 2010. Comparative studies of four different phenolic compounds on in vitro antioxidative activity and the preventive effect on lipid oxidation of fish oil emulsion and fish mince. Food Chemistry, 119, 123-132.
[33] Gardner, H.W., 1979. Lipid hydroperoxides reactivity with proteins and amino acids a review. Journal of Agricultural and Food Chemistry, 43, 651-656.
[34] Popova, T., Marinova, P., Vasileva, V., Gorinov, Y. and Lidji, K., 2009. Oxidative changes in lipids and proteins in beef during storage. Archiva Zootechnica, 12(3), 30-38.
[35] Baron, C.P., Kjaergard, I.V.H., Jessen, F. and Jacobsen, C., 2007. Protein and lipid oxidation during frozen storage of rainbow trout (Oncorhydchus mykiss). Journal of Agricultural and Food Chemistry, 55, 8118-8125.
[36] Sabeena, F., Helene, D.G. and Charlotte, J., 2012. Potato peel extract as a natural antioxidant in chilled storage of minced hourse mackerel (Trachurus trachurus): effect on lipid and protein oxidation. Food Chemistry, 13, 842-851.
[37] Siebet, K.J., Troukhanova, N.V. and Lynn, P.Y., 1996. Nature of polyphenol-protein interactions. Journal of Agricultural and Food Chemistry, 44, 8-85.
[38] Zhang, R., Xiong, S. You, J., Hu, Y., Liu, R. and Yin, T., 2017. Effects of ozone treatments on the physicochemical changes of myofibrillar proteins from Silver Carp (Hypophthalmichthys molitrix) during frozen storage. Journal of Food Quality, 2017,
[39] Wang, T., Li, Z., Yuan, F., Lin, H. and Pavas, T.R., 2016. Effects of brown seaweed polyphenols, α‐tocopherol, and ascorbic acid on protein oxidation and textural properties of fish mince (Pagrosomus major) during frozen storage. Journal of the Science of Food and Agriculture, 97(4), 1102-1107.
[40] Bekhit, A.E.A., Giteru, S.G., Holman, B.W.B. and Hopkins, D.L., 2021. Total volatile basic nitrogen and trimethylamine in musclefoods: Potential formation pathways and effects on human health. Comprehensive Reviews in Food Science and Food Safety, 20, 3620-3666.
[41] Cheng, J.H., Sun, D.W. and Wei, Q., 2017. Enhancing visible and near-infrared hyperspectral imaging prediction of TVB-N level for fishfillet freshness evaluation by filtering optimal variables. Food Analytical Methods, 10(6),1888-1898.
[42] Khammuang, S. and Sarnthima, K., 2011. Antioxidant and antibacterial activities of selected varieties of Thai mango seed extract. Pakistan Journal of Pharmaceutical Sciences, 24, 37-42.