In Vitro Propagation and Genetic Fidelity Assessment of Hedychium longicornutum Griff. ex Baker, a Vulnerable Zingiberaceous Plant of Thailand
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Abstract
Hedychium longicornutum Griff. ex Baker is a Zingiberaceous plant that has been used for traditional medicines and ornamental purposes. Though this plant is a vulnerable plant of Thailand, it has a high potential to be promoted as a new economic ornamental plant due to its exotic inflorescences. However, the usage of this valuable plant on a large scale is restricted since the population of H. longicornutum in natural habitat is limited. This current study established a micropropagation protocol to ensure sustainable use of this plant for commercial and conservation purposes. In vitro axenic plants of H. longicornutum were initiated from seeds. Optimum shoot multiplication medium was evaluated by culturing leafy-shoot bases on Murashige and Skoog’s (MS) medium supplemented with 0-35 µM of N6-benzyladenine (BA) or thidiazuron (TDZ). BA supplemented media were better than those TDZ media since no abnormalities of shoot morphology, shoot elongation, and root regeneration were found. MS supplemented with 15 µM BA was revealed to be the most effective medium for shoot multiplication (9.75 leafy-shoots/explant with 9.54 cm height). Regenerated shoots spontaneously rooted on MS medium. The in vitro raised plants were successfully grown ex vitro and showed the highest survival rate (100%) on planting substrate consisting of 2 garden soil mixture: 1 smashed charcoal. The genetic fidelity of the micropropagated plants compared with mother plants grown in the field was assessed by random amplified polymorphic DNA (RAPD) technique. The results consistently demonstrated the genetic uniformity of the regenerants. This is the first report of an established in vitro true-to-true type propagation in H. longicornutum.
Keywords: Zingiberaceae; micropropagation; cytokinin; direct organogenesis; genetic fidelity
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E-mail: ngarmnij.chu@mahidol.edu
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References
Royal Botanic Gardens Kew, 2018. Hedychium J.Koenig. [online]. Available at: http://plantsoftheworldonline.org/taxon/urn:lsid:ipni.org:names:30080711-2.
Sakhanokho, H.F. and Rajasekaranb, K., 2010. Pollen biology of ornamental ginger (Hedychium spp. J. Koenig). Scientia Horticulturae, 125, 129-135.
Larsen, K. and Larsen, S., 2006. Ginger of Thailand. Chiang Mai: Queen Sirikit Botanic Garden, the Botanical Garden Organization.
Eusebio, J.E. and Umali, B.E., 2004. Inventory, documentation and status of medicinal plants research in Philippines. In: P.A. Batugal, J. Kanniah, L.S. Young and J.T. Oliver, eds., Medicinal Plants Research in Asia, Vol. 1: The Framework and Project Workplans, Selangor: International Plant Genetic Resource Institute-Regional office for Asia, the Pacific and Oceania (IPGRI-APO), pp.147-183.
Tavares, W.R., Barreto, M.D.C. and Seca, A.M.L., 2020. Uncharted source of medicinal products: the case of the Hedychium genus. Medicines, 7(23), https://doi.org/10.3390/medicines7050023.
Rodpradit, S., Songnun, K. and Shusuwanaruk, K., 2017. In vitro microrhizome induction in Hedychium stenopetalum Lodd. Acta Horticulturae, 1167, 163-168.
Behera, S., Kamila, P.K., Rout, K.K., Barik, D.P., Panda, P.C. and Naik, S.K., 2018. An efficient plant regeneration protocol of an industrially important plant, Hedychium coronarium J. Koenig and establishment of genetic and biochemical fidelity of the regenerants. Industrial Crops and Products, 126, 58-68.
Mohanty, P., Behera, S., Swain, S.S., Barik, D.P. and Naik, S.K., 2013. Micropropagation of Hedychium coronarium J. Koenig through rhizome bud. Physiology and Molecular Biology of Plants, 19(4), 605-610.
Chamchumroon, V., 2017. Threatened Plants in Thailand. Bangkok: Department of National Parks, Wildlife and Plant Conservation.
Sirirugsa, P., 1998. Thai Zingiberaceae: species diversity and their uses. Pure and Applied Chemistry, 70, 2111-2118.
Hodgkison, R., Balding, S.T., Akbar, Z. and Kunz, T., 2003. Roosting ecology and social organization of the spotted-winged fruit bat, Balionycteris maculata (Chiroptera: Pteropodidae), in a Malaysian lowland dipterocarp forest. Journal of Tropical Ecology, 19, 667-676.
Olander, S.B., 2020. Hedychium longicornutum. The IUCN Red List of Threatened Species 2020. [online] Available at: https://dx.doi.org/10.2305/IUCN.UK.2020-2.RLTS.T117356150A1242 83162.en.
Hu, X., Tan, J., Chen, J., Li, Y. and Huang, J., 2020. Efficient regeneration of Hedychium coronarium through protocorm-Like Bodies. Agronomy, 10(8), 1068, https://doi.org/10.3390/agronomy10081068.
George, E.F., Hall, M.A. and De-Klerk, G.J., 2008. Plant Propagation by Tissue Culture. 3rd ed. Dordrecht: Springer.
Parida, R., Mohanty, S. and Nayak, S., 2013. In vitro propagation of Hedychium coronarium Koen. through axillary bud proliferation. Plant Biosystems, 147(4), 905-912.
Behera, S., Kar, S.K., Rout, K.K., Barik, D.P., Panda, P.C. and Naik, S.K., 2019.
Assessment of genetic and biochemical fidelity of field-established Hedychium coronarium J. Koenig regenerated from axenic cotyledonary node on meta-topolin supplemented medium. Industrial Crops and Products, 134, 206-215.
Sakhanokho, H.F., Kelly, R.Y. and Rajasekaran, K. 2009. Somatic embryogenesis in Hedychium bousigonianum. HortScience, 44, 1487-1490.
Chuengpanya, R., Karoojee, S., Lekbamroong, M., Muangkroot, A. and Chuenboonngarm, N., 2020. Effect of N6-benzyladenine on in vitro propagation of Hedychium coccineum Buch.-Ham. ex Sm. and Hedychium ellipticum Buch.-Ham. ex Sm. Srinakharinwirot Science Journal, 36(2), 91-107.
Behera, S., Rout, K.K., Panda, P.C. and Naik, S.K., 2020. Production of non-embryogenic synthetic seeds for propagation and germplasm transfer of Hedychium coronarium J. Koenig. Journal of Applied Research on Medicinal and Aromatic Plants, 19, https://doi.org/10.1016/j.jarmap.2020.100271.
Bisht, S., Bisht, N.S. and Bhandari, S., 2012. In vitro plant regeneration from seedling explants of Hedychium coronarium J. Koenig. Journal of Medicinal Plants Research, 6(43), 5546-5551.
Huang, P.L. and Tsai, C.C., 2002. Micropropagation of Hedychium coronarium Koenig. via somatic embryogenesis. Journal of the Chinese Society for Horticultural Science, 48, 239-246.
Mohanty, S., Parida, R., Sahoo, S. and Nayak, S., 2014. In vitro conservation of nine medicinally and economically important species of Zingiberaceae from Eastern India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 84(3), 799-803.
Rungjindamai, C., Thammasiri, K., Chuenboonngarm, N. and Jenjittikul, T., 2014. Micropropagation of Hedychium coronarium. Acta Horticulturae, 1025, 223-229.
El-Sadat, N., Soliman, A. and El-Zeiny, O., 2020. The effect of plant growth regulators on shoot formation from corm explant of Hedychium coronarium. Scientific Journal of Flowers and Ornamental Plants, 7(4), 401-407.
Tu, H.Y., Zhang, A.L., Xiao, W. Lin, Y.R., Shi, J.H., Wu, Y.W., Wu, S.T., Zhong, C.H. and Mo, S.X., 2018. Induction and identification of tetraploid Hedychium coronarium through thin cell layer culture. Plant Cell, Tissue and Organ Culture, 135, 395-406.
Verma, M. and Bansal, Y.K., 2012. Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig. Indian Journal of Experimental Biology, 50, 904-909.
Verma, M. and Bansal, Y.K., 2013. Effect of additives on plant regeneration in Hedychium coronarium J. Koenig an endangered aromatic and medicinal herb. International Journal of Pharmaceutical Sciences Review and Research, 23, 105-110.
Verma, M. and Bansal, Y.K., 2014. Effect of a potent cytokinin thidiazuron (TDZ) on in vitro regeneration of Hedychium coronarium J. Koenig - a valuable medicinal plant. International Journal of Recent Biotechnology, 2(1), 38-44.
Wang, X., Hongyan, T. and Ailing, Z., 2016. Embryogenic callus induction and plant regeneration from Hedychium coronarium via somatic embryogenesis. Acta Horticulturae Sinica, 43, 1605-1612.
Klaharn, P., Pumisutapon, P., Songnun, K. and Rodpradit, S., 2019. Effects of BA and TDZ for in vitro shoot multiplication of three Hedychium species. Acta Horticulturae, 1298, 353-358.
Xiong, Y.H., Ma, G.H., Liu, N. and Huang, B.H., 2005. Tissue culture and rapid propagation of zygotic embryo of Hedychium forrestii. Guihaia, 25(3), 24l-244.
Dohling, S., 2012. In vitro propagation of Hedychium gardnerianum Sheppard ex Ker Gawl., an important ornamental plant. Keanean Journal of Science, 1, 31-35.
Sakhanokho, H.F., Kelly, R.Y. and Rajasekaran, K., 2008. First report of plant regeneration via somatic embryogenesis from shoot apex derived callus of Hedychium muluense. Journal of Crop Improvement, 21, 191-200.
Tripathi, B.K. and Bitaillon, C., 1985. In vitro plant regeneration of Hedychium roxburghii blume through rhizome-meristem culture. Plant Cell, Tissue and Organ Culture, 4(1), 11-17.
Giri, D. and Tamta, S., 2011. Effect of plant growth regulators (PGRs) on micropropagation of a vulnerable and high value medicinal plant Hedychium spicatum. African Journal of Biotechnology, 10(20), 4040-4045.
Giri, D. and Tamta, S., 2013. Induction of somatic embryogenesis in vulnerable medicinal plant Hedychium spicatum Buch-Ham ex Smith. Plant Tissue Culture and Biotechnology, 23(2), 147-155.
Koul, S., Raina, V. and Sharma, S.K., 2005. Conservation and propagation of high altitude medicinal and aromatic plant: Hedychium spicatum. Journal of Plant Biochemistry and Biotechnology, 14(1), 57-59.
Gordon, S.P., Chickarmane, V.S., Ohno, C. and Meyerowitz, E.M., 2009. Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem. Proceedings of the National Academy of Sciences of the United States of America, 106(38), 16529-16534.
Auer, C.A., Motyka, V., Brezinová, A. and Kamínek, M., 1999. Endogenous cytokinin accumulation and cytokinin oxidase activity during shoot organogenesis of Petunia hybrida. Physiologia Plantarum, 105(1), 141-147.
Ren, B., Zhu, Y., Zhang, J., Dong, S., Liu, P. and Zhao, B., 2016. Effects of spraying exogenous hormone 6-benzyladenine (6-BA) after waterlogging on grain yield and growth of summer maize. Field Crops Research, 188, 96-104.
Gavinlertvatana, P. and Prutpongse, P., 1991. Commercial micropropagation in Asia. In: P.C. Debergh and R.H. Zimmerman, eds. Micropropagation: Technology and Application. Dordrecht: Kluwer Academic Publishers, pp. 181-189.
Mok, M.C., Mok, D.W.S., Armstrong, D.J., Shudo, K., Isogai, Y. and Okamoto, T., 1982. Cytokinin activity of N-phenyl-N-1,2,3-thiadiazol-5-ylurea (thidiazuron). Phytochemistry, 21, 1509-1511.
Victor, J.M.R., Murthy, B.N.S., Murch, S.J., Krishnaraj, S. and Saxena, P.K., 1999. Role of endogenous purine metabolism in thidiazuron induced somatic embryogenesis of peanut (Arachis hypogea L). Plant Growth Regulation, 28, 41-47.
Genkov, T. and Ivanova, I., 1995. Effect of cytokinin-active phenyl urea derivatives on shoot multiplication, peroxidase and superoxide dismutase activities of in-vitro cultured carnation. Bulgarian Journal of Plant Physiology, 21(1), 73-83.
Mok, M.C., Mok, D., Turner, J. and Mujer, C., 1987. Biological and biochemical effects of cytokinins-active phenylurea derivatives in tissue culture systems. HortScience, 22(6), 1194-1197.
Bairu, M.W., Aremu, A.O. and Van Staden, J., 2011. Somaclonal variation in plants: causes and detection methods. Plant Growth Regulation, 63(2), 147-173.
Saha, M.M.R., Liang, Y., Cheng, J.J., Daroch, M., 2016. Astaxanthin-producing green microalga Haematococcus pluvialis: from single cell to high value commercial products. Frontiers in Plant Science, 7, https://doi.org/10.3389/fpls.2016.00531.
Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M. and Sadh, R.K., 2016. Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech, 6(1), https://doi.org/10.1007/s13205-016-0389-7.
Dewir, Y.H., Naidoo, Y. and da Silva, J.A.T., 2018. Thidiazuron-induced abnormalities in plant tissue cultures. Plant Cell Reports, 37(11), 1451-1470.
Saha, S., Kader, A., Sengupta, C. and Ghosh, P., 2012. In vitro propagation of Ocimum gratissimum L. (Lamiaceae) and its evaluation of genetic fidelity using RAPD marker. American Journal of Plant Sciences, 3, 64-74.
Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
Arzoo, K. and Biswas, S.K., 2013. Effect of plant extracts as seed treatments on growth parameters, seedlings mortality and biochemical changes in tomato. International Journal of Bio-resource and Stress Management, 4, 47-53.
Nwachukwu, E.O. and Umechuruba, C.I., 2001. Antifungal activities of some leaf extracts on seed-borne fungi of African yam, bean seed germination and seedling emergence. Journal of Applied Sciences and Environmental Management, 5(1), 29-32.
Beyaz, R., Ozgen, Y., Poyraz, I., Cavdar, A. and Yildiz, M., 2021. Effects of squirting cucumber fruit juice on seed germination and seedling growth in rapeseed. Fresenius Environmental Bulletin, 30(1), 48-53.
Carimi, F., Zottini, M., Formentin, E., Terzi, M. and Schiavo, F.L., 2003. Cytokinins: new apoptotic inducers in plants. Planta, 216(3), 413-421.
Saha, S., Mori, H. and Hattori, K., 2007. Synergistic effect of kinenin and benzyl adenine plays a vital role in high frequency regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria) in relation to ethylene production. Breeding Science, 57, 197-202.
Gaba, V.P., 2005. Plant growth regulators in plant tissue culture and development. In R.N. Trigiano and D.J. Gray, eds. Plant Development and Biotechnology. Florida: CRC Press, pp. 87-99.
Mok, M.C., Martin, R.C. and Mok, D.W., 2000. Cytokinins: biosynthesis metabolism and perception. In Vitro Cellular and Developmental Biology – Plant, 36(2), 102-107.
Bairu, M.W., Novák, O., Doležal, K. and Staden, J.V., 2011. Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments. Plant Growth Regulation, 63(2), 105-114.
Sim, G.E., Loh, C.S. and Goh, C.J., 2007. High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae). Plant Cell Reports, 26, 383-393.
Grzegorczyk-Karolak, I., Kuzma, L. and Wysokinska, H., 2015. The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina. Plant Cell, Tissue and Organ Culture, 122(3), 699-708.
Chuengpanya, R., Chuenboonngarm, N., Kongtook, N., Longsaward, R., Nopporncharoenkul, N., Muangkroot, A., Jenjittikul, T. and Soonthornchainaksaeng, P., 2019. In vitro propagation of Curcuma candida (Wall.) Techapr. and Škornick., a vulnerable plant of Thailand. Srinakharinwirot Science Journal, 35(2), 31-49.
Chandra, S., Bandopadhyay, R., Kumar, V. and Chandra, R., 2010. Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnology Letters, 32(9), 1199-1205.