Biosynthesis of Silver Nanoparticles from Truffles and Mushrooms and Their Applications as Nanodrugs

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Mustafa Nadhim Owaid*


This study aims to seek a new bio-agent as a reducer to synthesize silver nanoparticles (AgNPs). Dried fruiting bodies of truffles were used to mycosynthesize AgNPs, which were described by optical vision, UV-visible spectroscopy, SEM, AFM, Zetasizer, Granularity Cumulation Distribution, and FTIR analyses. The brown color is a sign of mycosynthesizing AgNPs. The UV-visible spectrum exhibited an absorption peak of 450 nm. Images of SEM showed that most of the AgNPs have an average of sizes reached 72 nm with irregular and spherical shapes. The concentration 10 mg/well exhibited 9.5 mm, and 20 mm toward Klebsiella, and P. aeruginosa, respectively. The synergism effect between gentamicin antibiotic and desert truffle-synthesized AgNPs is remarkable to increase the antibacterial activity. The zone of inhibition recorded 18 mm, 18.3 mm, and 20 mm by the conjugation between gentamicin and 5 mg/well of AgNPs compared with 21 mm, 20.6 mm and 23 mm by the conjugation between gentamicin and 10 mg/well against S. aureus, Klebsiella, and P. aeruginosa, respectively.

Keywords: green chemistry; antibacterial activity; Tirmania; mushroom; AgNPs

*Corresponding author: Tel.: (+964) 7902651440




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Alizadeh, V., Eimani, B.G. and Amjady, F., 2018. Genomic effect of silver nanoparticles in Staphylococcus aureus bacteria. Journal of Water and Environmental Nanotechnology, 3(1), 51-57.

Amendola, V., Bakr, O.M. and Stellacci, F., 2010. A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: Effect of shape, size, structure, and assembly. Plasmonics, 5(1), 85-97.

Awad, M.A.G., Al Olayan, E.M., Alsaif, S.S.A., Siddiqui, M.I. and Elkhadragy, M.F., 2017. Synthesis of Metal Nanoparticles Using an Extract of Terfeziaceae. US. Pat. 9,637,807.

Bokhary, H., Suleiman, A. and Basalah, M., 1989. The fatty acid components of the desert truffle ‘AI Kamah’ of Saudi Arabia. Journal of Food Protection, 52, 668-669.

Bradai, L., Bissati, S. and Chenchouni, H., 2014. Desert truffles of the North Algerian Sahara: diversity and bioecology. Emirates Journal of Food and Agriculture, 26, 429-439.

Dogan, H. and Aydin, S., 2013. Determination of antimicrobial effect, antioxidant activity and phenolic contents of desert truffle in Turkey. Journal of Research in Pharmacy,10(4), 52-58.

Dogan, H., Duman, R., Ozkalp, B. and Aydin, S., 2013. Antimicrobial activities of some mushrooms in Turkey. Pharmaceutical Biology, 51(6), 707-711.

Fayaz, A., Balaji, K., Girilal, M., Yadav, R., Kalaichelvan, P. and Venketesan, R., 2010. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against Gram positive and Gram-negative bacteria. Nanomedicine, 6, 103-109.

Gajos, M., Ryszka, F. and Geistlinger, J., 2014. The therapeutic potential of truffle fungi: A patent survey. Acta Mycologica, 49, 305-318.

Goh, E.G., Xu, X. and McCormick, P.G., 2014. Effect of particle size on the UV absorbance of zinc oxide nanoparticles. Scripta Materialia, 78-79, 49-52.

Janakat, S., Al-Fakhiri, S. and Sallal, A.-K., 2004. A promising peptide antibiotic from Terfezia claveryi aqueous extract against Staphylococcus aureus in vitro. Phytotherapy Research, 18, 810-813.

Janakat, S., Al-Fakhiri, S. and Sallal, A.K., 2005. Evaluation of antibacterial activity of aqueous and methanolic extracts of the truffle Terfezia claveryi against Pseudomonas aeruginosa. Saudi Medical Journal, 26(6), 952-955.

Janakat, S. and Nassar, M., 2010. Hepatoprotective activity of desert truffle (Terfezia claveryi) in comparison with the effect of Nigella sativa in the rat. Pakistan Journal of Nutrition, 9(1), 52-56.

Khadri, H., Aldebasi, Y.H. and Riazunnisa, K., 2017. Truffle mediated ( Terfezia claveryi) synthesis of silver nanoparticles and its potential cytotoxicity in human breast cancer cells (MCF-7). African Journal of Biotechnology, 16(22), 1278-1284.

Meghwal, M. and Goswami, T., 2012. Chemical composition, nutritional, medicinal and functional properties of black pepper: A review. Scientific Reports, 1(2), 11-15.

Muhsin, T.M. and Hachim, A.K., 2016. Characterization and antibacterial efficacy of mycosynthesized silver nanoparticles from the desert truffle Tirmania nivea. Qatar Foundation Annual Research Conference Proceedings, Doha, Qatar, March 22-23, 2016,

Muhsin, T. and Hachim, A., 2014. Mycosynthesis and characterization of silver nanoparticles and their activity against some human pathogenic bacteria. World Journal of Microbiology and Biotechnology, 30(7), 2081-2090.

Owaid, M.N., 2016. Biodiversity and bioecology of Iraqi desert truffles (Pezizaceae) during season 2014. Journal of Aridland Agriculture. 2, 22-25.

Owaid, M.N., 2018. Bioecology and uses of desert truffles (Pezizales) in the middle east. Walailak Journal of Science and Technology, 15(3), 179-188.

Owaid, M.N., Al-Saeedi, S.S.S. and Abed, I.A., 2017. Study on UV-visible for detection of biosynthesis of silver nanoparticles by oyster mushroom’s extracts. Journal of Water and Environmental Nanotechnology, 2(1), 66-70.

Owaid, M.N. and Ibraheem, I.J., 2017. Mycosynthesis of nanoparticles using edible and medicinal mushrooms. European Journal of Nanomedicine. 9(1), 5-23.

Owaid, M.N., Muslim, R.F. and Hamad, H.A., 2018. Mycosynthesis of silver nanoparticles using Terminia sp. desert truffle, Pezizaceae, and their antibacterial activity. Jordan Journal of Biological Sciences, 11(4), 401-405.

Owaid, M.N., Naeem, G.A., Muslim, R.F. and Oleiwi, R.S., 2020. Synthesis, characterization and antitumor efficacy of silver nanoparticle from Agaricus bisporus pileus, Basidiomycota. Walailak Journal of Science and Technology, 17(2), 75-87.

Owaid, M.N., Raman, J., Lakshmanan, H., Al-Saeedi, S.S.S., Sabaratnam, V. and Ali, I.A., 2015. Mycosynthesis of silver nanoparticles by Pleurotus cornucopiae var. citrinopileatus and its inhibitory effects against Candida sp. Materials Letters. 153, 186-190.

Petryayeva, E. and Krull, U.J., 2011. Localized surface plasmon resonance: Nanostructures, bioassays and biosensing- A review. Analytica Chimica Acta, 706(1), 8-24.

Pingale, S.S., Rupanar, S.V. and Chaskar, M., 2018. Plant- mediated biosynthesis of silver nanoparticles from Gymnema sylvestre and their use in phtodegradation of methyl orange dye. Journal of Water and Environmental Nanotechnology, 3(2), 106-115.

Radif, H.M., Owaid, M.N. and Al-bahrani, R.M., 2019. Evaluation influence of extract of Terfezia claveryi desert truffle, Pezizaceaem against Streptococcus pneumoniae, in vivo. Malaysian Journal of Biochemistry and Molecular Biology, 2, 21-25.

Shahverdi, A.R., Fakhimi, A., Shahverdi, H.R. and Minaian, S., 2007. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine: Nanotechnology, Biology, and Medicine, 3(2), 168-171.

Tel-Çayan, G., Devecİ, E., Çayan, F. and Duru, M.E., 2018. Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota truffle fungi from Turkey. Marmara Pharmaceutical Journal, 22(1), 59-65.

Vasil’kov, A.Y., Dovnar, R.I., Smotryn, S.M., Iaskevich, N.N. and Naumkin, A.V., 2018. Plasmon resonance of silver nanoparticles as a method of increasing their antibacterial action. Antibiotics, 7(3), 80,

Vijayan, S., Koilaparambil, D., George, T.K. and Shaikmoideen, J.M., 2016. Antibacterial and cytotoxicity studies of silver nanoparticles synthesized by endophytic Fusarium solani isolated from Withania somenifera (L.). Journal of Water and Environmental Nanotechnology, 1(2), 91-103.