Inhibitory Effect of Water Soluble Fraction of Monascus-Fermented Rice on Lipid Accumulation in 3T3 L1 Adipocyte
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Abstract
Excessive lipid accumulation in the body causes people to become overweight and obese, conditions that are associated with an amplified risk of serious diseases. The fungi of genus Monascus produce various secondary metabolites such as monacolins, citrinin and fungal pigments, which are water-insoluble and have inhibitory potency for the lipid accumulation in adipocytes. However, water-soluble adipogenesis inhibitors derived from Monascus-fermented products have not yet been reported. In this study, we investigated the inhibitory activity against intracellular lipid accumulation of water-soluble fractions of Monascus-fermented red mold rice (RMR) and red mold barley (RMB) on murine 3T3-L1 cells. Water soluble fractions of ten different Monascus strains were used and the inhibitory activity of their water-soluble fractions on lipid accumulation by differentiated 3T3-L1 cells was evaluated for 8 days using oil red staining. The water-soluble fraction from Monascus pilosus NBRC4507 fermented RMR cultivated at 30°C for 14 days was selected since it showed comparatively the lowest relative lipid accumulation (62±1.2%), which indicated the highest inhibitory activity of lipid accumulation in adipocytes. To study the presence of monacolin and citrinin in the water soluble fractions, thin-layer chromatography was done and the results showed that the water-soluble fractions tested were free from both monacolin and citrinin. Therefore, the present study strongly suggested that the water-soluble components, except for monacolin and citrinin, in the water soluble fraction obtained from Monascus pilosus NBRC4507-fermented rice can be used as functional food material to control overweight and obesity.
Keywords: lipid accumulation; Monascus; red mold rice; water soluble fraction; cultivation conditions
*Corresponding author: Tel.: (+94) 773311010
E-mail: mnkaumal@sci.cmb.ac.lk
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References
Ailhaud, G., 1982. Adipose cell differentiation in culture. Molecular and Cell Biochemistry, 49, 17-31.
Kopelman, P.G., 2000. Obesity as a medical problem. Nature, 404, 635-643.
Bray, G.A., 2004. Medical consequences of obesity. The journal of Clinical Endocrinology and Metabolism, 89, 2583-2589.
Rosen, E.D. and Spiegelman, B.M., 2000. Molecular regulation of adipogenesis. Annual Review of Cell and Developmental Biology, 16, 145-171.
Arunachalam, C. and Narmadhapriya, D., 2011. Monascus fermented rice and its beneficial aspects: a new review. Asian Journal of Pharmaceutical and Clinical Research, 4, 29-31.
Erdoğrul, Ö. and Azirak, S., 2004. Review of the studies on the red yeast rice (Monascus purpureus). Turkish Journal of Biotechnology, 2, 37-49.
Feng, Y., Shao, Y. and Chen, F., 2012. Monascus pigments. Applied Microbiology and Biotechnology, 96, 1421-1440.
Yasuda, M., Tachibana, S. and Kuba-Miyara, M., 2012. Biochemical aspects of red koji and tofuyo prepared using Monascus fungi. Applied Microbiology and Biotechnology, 96, 49-60.
Chen, W.P., Ho, B.Y., Lee, C.L., Lee, C.H. and Pan, T.M., 2008. Red mold rice prevents the development of obesity, dyslipidemia and hyperinsulinemia induced by high-fat diet. International Journal of Obesity, 32(11), 1694-1704.
Jeon, T., Hwang, S.G., Hirai, S., Matsui, T., Yano, H., Kawada, T. and Lim, B.O., 2004. Park DK. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sciences, 75, 3195-3203.
Lee, C.L., Wen, J.Y., Hsu, Y.W. and Pan, T.M., 2013. Monascus-fermented yellow pigments monascin and ankaflavin showed antiobesity effect via the suppression of differentiation and lipogenesis in obese rats fed a high-fat diet. Journal of Agricultural and Food Chemistry, 61, 1493-1500.
Endo, A., 1979. Monacolin K, a new hypocholesterolemic agent produced by a Monascus species. The Journal of Antibiotics, 32, 852-854.
Endo, A., 1980. Monacolin K, a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase. The Journal of Antibiotics, 33, 334-336.
Wang, J., Lu, Z., Chi, J., Wang, W., Su, M., Kou, W., Yu, P., Yu, L., Chen, L., Zhu, J.S. and Chang, J., 1997. Multicenter clinical trial of the serum lipid-lowering effects of a Monascus purpureus (red yeast) rice preparation from traditional Chinese medicine. Current Therapeutic Research, Clinical and Experimental, 58(12), 946-978.
Kalaivani, M., Sabitha, R. and Kalaiselvan, V., 2010. Health benefits and clinical impact of major nutrient, red yeast rice: a review. Food and Bioprocess Technology, 3, 333-339.
Blanc, P.J., Laussac, J.P., Bars, J.L., Bars, P.L., Loret, M.O., Pareilleux, A., Prome, D., Prome, J.C., Santerre, A.L. and Goma, G., 1995. Characterization of monascidin A from Monascus as citrinin. International Journal of Food Microbiology, 27, 201-213.
Sabater-Vilar, M., Maas, R.F.M. and Fink-Gremmels, J., 1999. Mutagenicity of commercial Monascus fermentation products and the role of citrinin contamination. Mutation Research, 444, 7-16.
Liu, B.H., Wu, T.S., Su, M.C. and Yu, F.Y., 2005. Evaluation of citrinin occurrence and cytotoxicity in Monascus fermentation products. Journal of Agricultural and Food Chemistry, 53, 170-175.
Endo, A. and Kuroda, M., 1976. Citrinin, an inhibitor of cholesterol synthesis. The Journal of Antibiotics, 29, 841-843.
McIntosh, G.H., Whyte, J., McArlthur, R. and Nestel, P.J., 1991. Barley and wheat foods: influence on plasma cholesterol concentrations in hypercholesterolemic men. American Journal of Clinical Nutrition, 53, 1205-1209.
Lambo, A.M., Öste, R. and Nyman, M.E.G.L., 2005. Dietary fibre in fermented oat and barley -glucan rich concentrates. Food Chemistry, 89, 283-293.
Talati, R., Baker, W.L., Pabilonia, M.S., White, C.M. and Coleman, C.I., 2009. The effects of barley-derived soluble fiber on serum lipids. Annals of Family Medicine. 7, 157-163.
Roh, C. and Jung, U., 2012. Screening of crude plant extracts with anti-obesity activity. International Journal of Molecular Sciences, 13, 1710-1719.
Jaivel, N. and Marimuthu, P., 2010. Isolation and screening of lovastatin producing microorganisms. International Journal of Engineering, Science and Technology, 2, 2607-2611.
Pepeljnjak, S., Šegvić, M. and Ožegović, L., 2002. Citrininotoxinogenicity of Penicillium spp. isolated from decaying apples. Brazilian Journal of Microbiology, 33, 134-137.
Jou, P.C., Ho, B.Y., Hsu, Y.W. and Pan, T.M., 2010. The effect of Monascus secondary polyketide metabolites, monascin and ankaflavin, on adipogenesis and lipolysis activity in 3T3-L1. Journal of Agricultural and Food Chemistry, 58, 12703-12709.
Tokinaga, K., Oeda, T., Suzuki, Y. and Matsushima, Y., 2006. HMG-CoA reductase inhibitors (statins) might cause high elevations of creatine phosphokinase (CK) in patients with unnoticed hypothyroidism. Endocrine Journal, 53, 401-405.
Wang, J.J., Lee, C.L. and Pan, T.M., 2003. Improvement of monacolin K, -aminobutyric acid and citrinin production ratio as a function of environmental conditions of Monascus purpureus NTU 601. Journal of Industrial Microbiology and Biotechnology, 30, 669-676.
Hsu, F.L., Wang, P.M., Lu, S.Y. and Wu, W.T., 2002. A combined solid-state and submerged cultivation integrated with adsorptive product extraction for production of Monascus red pigments. Bioprocess and Biosystem Engineering, 25, 165-168.
Johns, M.R. and Stuart, D.M., 1991. Production of pigments by Monascus purpureus in solid culture. Journal of Industrial Microbiology, 8(1), 23-28.
Wen, Q., Cao, X., Chen, Z., Xiong, X., Liu, J., Cheng, Z., Zheng, Z., Long, C., Zheng, B. and Huang, Z., 2020. An overview of Monascus fermentation process for monacolin K production. Open Chemistry, 18, 10-21.
Hong, S.Y., Oh, J.H. and Lee, I., 2011. Simultaneous enrichment of deglycosylated ginsenosides and monacolin K in red ginseng by fermentation with Monascus pilosus. Bioscience Biotechnology and Biochemistry, 75, 1490-1495.
Priatni, S., Damayanti, S., Saraswaty, V., Ratnaningrum, D. and Singgih, M., 2014. The utilization of solid substrates on Monascus fermentation for anticholesterol agent production. Procedia Chemistry, 9, 34-39.
Carvalho, J.C., Oishi, B.O., Woiciechowski, A.L., Pandey, A., Babith, S. and Soccolm, C.R., 2007. Effect of substrates on the production of Monascus biopigments by solid-state fermentation and pigment extraction using different solvents. Indian Journal of Biotechnology, 6, 194-199.
Qureshi, M.J., Mallikarjun, C. and Kian, W.G., 2015. Enhancement of solubility and therapeutic potential of poorly soluble lovastatin by SMEDDS formulation adsorbed on directly compressed spray dried magnesium aluminometasilicate liquid loadable tablets: A study in diet induced hyperlipidemic rabbits. Asian Journal of Pharmaceutical Science, 10, 40-56.
European Commission, 2014. Commission Regulation (EU) No 212/2014 of 6 March 2014 amending Regulation (EC) No 1881/2006 as regards maximum levels of the contaminant citrinin in food supplements based on rice fermented with red yeast Monascus purpureus. EFSA Journal, 67, 3-4.
Urraca, J.L., Huertas-Pérez, J.F., Cazorla, G.A., Gracia-Mora, J., García-Campaña, A.M. and Moreno-Bondi, M.C., 2016. Development of magnetic molecularly imprinted polymers for selective extraction: Determination of citrinin in rice samples by liquid chromatography with UV diode array detection. Analytical and Bioanalytical Chemistry, 408, 3033-3042.