Chemical Analysis and Bioactivities of Gaultheria procumbens L. Essential Oil: Herbicidal, Antioxidant and Antibacterial Activities
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Abstract
The chemical composition of wintergreen (Gaultheria procumbens L.) essential oil (WEO) was identified by gas chromatography-mass spectrometry (GC-MS) and evaluated for its biological activities. Methyl salicylate (100%) was the main compound in the wintergreen essential oil. The herbicidal efficacy of the WEO was confirmed by germination inhibition, radical and shoot length reduction, and phytotoxicity assessment with Echinochloa crus–galli and Amaranthus tricolor seedlings. The WEO significantly reduced the germination and seedling growth of both weeds. It showed highly significant inhibition of seed germination (93.37%) and seedling growth of A. tricolor at the highest dose (6 µL/petri dish) assayed. The WEO was formulated as an emulsifiable concentrate (EC-EO) for post-emergence application, and was applied at a range of concentrations from 10 to 80 mL/L. Both treated plant leaves appeared wilted and slightly discolored within 1 day of application (DAA). The visible weed control efficacy was most remarkable at 80 mL/L treatment, which was a level that ultimately killed the seedlings of both species at 3 DAA, suggesting promising herbicidal potential for the WEO. The WEO showed weak scavenging activity with a high IC50 value (IC50 >2 104 and >5 104 ppm) for DPPH scavenging and metal chelating assays, respectively. The WEO showed moderate antibacterial activity, and its zones of inhibition against bacterial test strains were 7.90±0.8 mm and 23.9±0.9 mm for Escherichia coli TISTR 780 and Staphylococcus aureus TISTR 1466, respectively. Therefore, the results suggest the possibility of using WEO as an active ingredient to produce natural herbicides.
Keywords: essential oil; EC-EO; Gaultheria procumbens; bioactivity; herbicidal activity; antibacterial activity; antioxidant activity
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E-mail: nutcha.manichart@gmail.com
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References
Bakhtiarizade, M. and Souri, M.K., 2019. Beneficial effects of rosemary, thyme and tarragon essential oils on postharvest decay of Valencia oranges. Chemical and Biological Technologies in Agriculture, 6(1), DOI: 10.1186/s40538-019-0146-3.
Sule, R.O., Condon, L. and Gomes, A.V., 2022. A common feature of pesticides: oxidative stress—the role of oxidative stress in pesticide-induced toxicity. Oxidative Medicine and Cellular Longevity, 2022, DOI: 10.1155/2022/5563759.
Singh, H.P., Batish, D.R. and Kohli, R.K., 2002. Allelopathic effect of two volatile monoterpenes against bill goat weed (Ageratum conyzoides L.). Crop Protection, 21(4), 347-350.
Singh, N. and Sheikh, J., 2022. Novel chitosan-gelatin microcapsules containing rosemary essential oil for the preparation of bioactive and protective linen. Industrial Crops and Products, 178, DOI: 10.1016/j.indcrop.2022.114549.
Singh, H., Batish, D., Setia, N. and Kohli, R., 2005. Herbicidal activity of volatile oils from Eucalyptus citriodora against Parthenium hysterophorus. Annals of Applied Biology, 146(1), 89-94.
Asbahani, A.E., Miladi, K., Badri, W., Sala, M., Addi, E.H.A., Casabianca, H., Mousadik, A.E., Hartmann, D., Jilale, A., Renaud, F.N.R. and Elaissari, A., 2015. Essential oils: From extraction to encapsulation. International Journal of Pharmaceutics, 483(1-2), 220-243.
Ghanbari, M., Souri, M.K. and Omidbaigi, R., 2017. Chemical composition of the essential oil of Achillea millefolium L. from north-west of Iran. Zeitschrift für Arznei- and Gewürzpflanzen, 22(2), 69-71.
Yahyaoui, M., Gordobil, O., Díaz, R.H., Abderrabba, M. and Labidi, J., 2016. Development of novel antimicrobial films based on poly(lactic acid) and essential oils. Reactive and Functional Polymers, 109, 1-8.
Nikolić, M., Marković, T., Mojović, M., Pejin, B., Savić, A., Perić, T., Marković, D., Stević, T. and Soković, M., 2013. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. Industrial Crops and Products, 49, 561-567.
Ribnicky, D.M., Poulev, A. and Raskin, I., 2003. The determination of salicylates in Gaultheria procumbens for use as a natural aspirin alternative. Journal of Nutraceuticals Functional and Medical Foods, 4, 39-52.
Cong, F., Joshi, K.R., Devkota, H.P., Watanabe, T. and Yahara, S., 2015. Dhasingreoside: new flavonoid from the stems and leaves of Gaultheria fragrantissima. Natural Product Research, 29, 1442-1448.
Singh, V., Gunjan and Ali, M., 2018. Isolation of volatile constituents and biological studies of aerial parts of Gaultheria procumbens L. International Journal of Green Pharmac, 11(4), DOI: 10.22377/ijgp.v11io4.1359.
Mulqueen, P., 2003. Recent advanced in agrochemical formulation. Advances in Colloid and Interface Science, 106(1-3), 83-107.
Aas, J.A., Paster, B.J., Stokes, L.N., Olsen, I. and Dewhirst, F.E., 2005. Defining the normal bacterial flora of the oral cavity. Journal of Clinical Microbiology, 43(11), 5721-5732.
Mele, J., Remmen, H.V., Vijg, J. and Richardson, A., 2006. Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase. Antioxidants and Redox Signaling, 8(3-4), 628-638.
Laosinwattana, C., Wichittrakarn, P. and Teerarak, M., 2018. Chemical composition and herbicidal action of essential oil from Tagetes erecta L. leaves. Industrial Crops and Products, 126, 129-134.
Laosinwattana, C., Poonpaiboonpipata, T., Teerarak, M., Phuwiwat, W., Mongkolaussavaratana, T. and Charoenying, P., 2009. Allelopathic potential of Chinese rice flower (Aglaia odorata Lour.) as organic herbicide. Allelopathy Journal, 24(1), 45-54.
Chotsaeng, N., Laosinwattana, C. and Charoenying, P., 2018. Inhibitory effects of a variety of aldehydes on Amaranthus tricolor L. and Echinochloa crus-galli (L.) Beauv. Molecules, 23, 471-485.
Brazilian Society of Weed Science—SBCPD, 1995. Procedures for Installation, Evaluation and Analysis of Experiments with Herbicides. Londrina: Brazilian Weed Science Society.
Ebrahimzadeh, M., Nabavi, S., Nabavi, S., Bahramian, F. and Bekhradnia, R., 2008. Antioxidant and free radical scavenging activity. Pakistan Journal of Pharmaceutical Sciences, 23, 29-34.
Jamuna, S., Paulsamy, S. and Karthika, K., 2012. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). Journal of Applied Pharmaceutical Science, 2(7), 149-154.
Kujur, A., Kiran, S., Dubey, N.K. and Prakash, B., 2017. Microencapsulation of Gaultheria procumbens essential oil using chitosan-cinnamic acid microgel: Improvement of antimicrobial activity, stability and mode of action. LWT, 86, 132-138.
Singh, V. and Ali, M., 2017. Isolation of volatile constituents and biological studies of aerial parts of Gaultheria procumbens L. International Journal of Green Pharmacy, 11(2), 784-788.
Ibáñez, M.D. and Blázquez, M.A., 2019. Tea tree and wintergreen essential oils in the management of the invasive species Cortaderia selloana and Nicotiana glauca. Journal of Plant Protection Research, 59(2), 160-169.
Pribitkin, E. and Boger, G., 2001. Herbal therapy: what every facial plastic surgeon must know. Archives of Facial Plastic Surgery, 3, 127-132.
Poonpaiboonpipata, T., Pangnakorn, U., Suvunnamek, U., Teerarak, M., Charoenying, P., and Laosinwattana, C., 2013. Phytotoxic effects of essential oil from Cymbopogon citratus and its physiological mechanisms on barnyardgrass (Echinochloa crus-galli). Industrial Crops and Products, 41, 403-407.
Mustafa, I.F. and Hussein, M.Z., 2020. Synthesis and technology of nanoemulsion-based pesticide formulation. Nanomaterials (Basel), 10(8), DOI: 10.3390/nano10081608.
Laosinwattana, C., Boonleom, C., Teerarak, M., Thitavasanta, S. and Charoenying, P., 2010. Potential allelopathic effects of Suregada multiflorum and the influence of soil type on its residue’s efficacy. Weed Biology and Management, 10, 153-159.
Charoenying P., Teerarak, M. and Laosinwattana, C. 2010. An allelopathic substance isolated from Zanthoxylum limonella Alston fruit. Scientia Horticulturae, 125, 411-416.
Souri, M.K. and Bakhtiarizade, M., 2019. Biostimulation effects of rosemary essential oil on growth and nutrient uptake of tomato seedlings. Scientia Horticulturae, 243, 472-476.
Laosinwattana, C., Teerarak, M. and Charoenying, P., 2012. Effects of Aglaia odorata granules on the seedling growth of major maize weeds and the influence of soil type on the granule residue’s efficacy. Weed Biology and Management, 12, 117-122.
Cordeau, S., Triolet, M., Wayman, S., Steinberg, C. and Guillemin, J.-P., 2016. Bioherbicides: Dead in the water? A review of the existing products for integrated weed management. Crop Protection, 87, 44-49.
Ibáñez, M.D. and Blázquez, M.A., 2018. Phytotoxicity of essential oils on selected weeds: Potential hazard on food crops. Plants (Basel), 7(4), DOI: 10.3390/plants7040079.
Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology, 94(3), 23-53.
Martucci, J.F., Gende, L.B., Neira, L.M. and Ruseckaite, R.A., 2015. Oregano and lavender essential oils as antioxidant and antimicrobial additives of biogenic gelatin films. Industrial Crops and Products, 71, 205-213.
Raut, J.S. and Karuppayil, S.M., 2014. A status review on the medicinal properties of essential oils. Industrial Crops and Products, 62, 250-264.
Chouhan, S., Sharma, K. and Guleria, S., 2017. Antimicrobial activity of some essential oils—present status and future perspectives. Medicines, 4(3), 58-79.