Sericin and Pineapple Fruit Extract as Natural Agents Inhibiting Browning and Improving Antioxidant Activity in Fresh-cut Ripe Mangoes cv. Nam Dok Mai

Main Article Content

Mathurot Kumthongwathana
Suriyan Supapvanich
Nutthachai Pongprasert
Chalermchai Wongs-Aree
Panida Boonyaritthongchai

Abstract

Recently, the use of natural agents to improve quality has attracted a lot of attention on fresh-cut produce and minimally processed fruit. The aims of this work were to investigate the efficiency of sericin and fresh pineapple juice (selected natural agents) on browning inhibition and antioxidant activity of fresh-cut ‘Nam Dok Mai’ mangoes during refrigeration (4°C). Based on a preliminary study and our previous work, 2% sericin (S) and 50% pineapple juice (PJ) were selected. Biological parameters related to enzymatic browning incidence, antioxidants and antioxidant enzyme activity of the fresh-cut mangoes immersed in 2% S, 50% PJ and 50% PJ incorporated with 2% S (PJ+S) were investigated.  Immersion in S, PJ or PJ+S inhibited enzymatic browning due to delaying of discoloration, lowering of browning index (BI) and increases in polyphenol oxidase (PPO) and peroxidase (POD) activities compared to control treatment. The lowest PPO and POD activities and total phenolic content were found in the S treated fresh-cut mangoes. PJ+S treated fresh-cut mangoes had antioxidant activities higher than other treatment. All treatments enhanced the activities of antioxidant enzymes, especially PJ immersion. Therefore, S and PJ show potential as natural agents that can inhibit browning and improve antioxidant activity in fresh-cut mangoes during refrigeration.

Article Details

Section
Original Research Articles

References

Trade Policy and Strategy Office (TPSO), Thailand, 2022. Analysis of Thai-Region Trading Economic Situation. [online] Available at: http://www.tpso.moc.go.th/sites/default/files/wiekhraaahesrsthkicchphuumiphaakhpracchameduuenemsaay.pdf.

Ulloa, J.A., dela Mora, E.E.V., Escalona, H.B., Díaz-Jiménez, L., Hernández-Tinoco, A. and Ciatej, A.C., 2004. Color and texture properties of minimally processed mango (Mangifera indica) segments by hurdle technology, auto stabilized in glass jars. 2004 IFT (Institute of Food Technologist) Annual Meeting and Food Expo, Las Vegas, USA. July 13-16, 2004, p. 216.

Baldwin, E.A., Nisperos, M.O. and Baker, R.A., 1995. Edible coatings for lightly processed fruits and vegetables. HortScience, 30, 35-38.

Moon, K.M., Lee, B., Cho, W., Lee, B., Kim, C.Y. and Ma, J.Y., 2018. Swertiajaponin as an anti-browning and antioxidant flavonoid. Food Chemistry, 252, 207-214.

Corzo-Martínez, M., Corzo, N., Villamiel, M. and del Castillo, M.D., 2012. Browning reactions. Food Biochemistry and Food Processing. 2nd ed. New Jersey, John Wiley and Sons, pp. 56-71.

Lim, W.Y., Cheun, C.F. and Wong, C.W., 2019. Inhibition of enzymatic browning in sweet potato (Ipomoea batatas (L.)) with chemical and natural anti‐browning agents. Journal of Food Processing and Preservation, 43(11), https://doi/org/10.1111/fpp.14195.

Liu, X., Yang, Q., Lu, Y., Li, Y., Li, T., Zhou, B. and Qiao, L., 2019. Effect of purslane (Portulaca oleracea L.) extract on anti-browning of fresh-cut potato slices during storage. Food Chemistry, 283, 445-453.

Brütsch, L., Rugiero, S., Serrano, S., Städeli, C., Windhab, E., Fischer, P. and Kuster, S., 2018. Targeted inhibition of enzymatic browning in wheat pastry dough. Journal of Agricultural and Food Chemistry, 66, 12353-12360.

Lee, M.K., Kim, Y.M., Kim, N.Y., Kim, G.N., Kim, S.H., Bang, K.S. and Park, I., 2002. Prevention of browning in potato with a heat-treated onion extract. Bioscience, Biotechnology and Biochemistry, 66, 856-858.

Supapvanich, S., Mitrsang, P., Srinorkham, P., Boonyaritthongchai, P. and Wongs-Aree, C., 2016. Effects of fresh Aloe vera gel coating on browning alleviation of fresh cut wax apple (Syzygium samarangenese) fruit cv. Taaptimjaan. Journal of Food Science and Technology, 53(6), 2844-2850.

Thanakorn, K. 2017. Effect of Sericin Coating and Pineapple Juice on Browning Reduction and Quality Changes of Fresh Cut Mango cv. Nam Dok Mai. M.Sc. King Mongkut’s University of Technology Thonburi, Bangkok, Thailand.

Chaisakdanugull, C., Theerakulkait, C. and Wrolstad, R.E., 2007. Pineapple juice and its fractions in enzymatic browning inhibition of banana. Journal of Agricultural and Food Chemistry, 55, 4252-4257.

Rattanathanalerk, M., Chiewchan, N. and Srichumpoung, W., 2005. Effect of thermal processing on the quality loss of pineapple juice. Journal of Food Engineering, 66, 259-265.

Food and Drug Administration (FDA). Notice of Inventory. [online] Available at: http://www.fda.gov/downloads/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/UCM267039.

Goetghebeur, M. and Kermasha, S., 1996. Inhibition of polyphenol oxidase by copper-metallothionein from Aspergillus niger. Journal of Photochemistry and Photobiology, 42, 935-942.

Chimvaree, C., Wongs-Aree, C., Supapvanich, S., Charoenrat, T., Tepsorn, R. and Boonyaritthongchai, P., 2019. Effect of sericin coating on reducing browning of fresh-cut mango cv. ‘Nam Dok Mai No. 4’. Agriculture and Natural Resources, 53, 521-526.

Palou, E., López-Malo, A., Barbosa-Cánovas, G.V., Welti-Chanes, J. and Swanson, B.G., 1999. Polyphenoloxidase activity and color of blanched and high hydrostatic pressure treated banana Puree. Journal of Food Science, 64, 42-45.

Slinkard, K. and Singleton, T., 1997. Total phenolic analyses: Automation and comparison with manual method. American Journal of Ecology and Viticulture, 28, 49-55.

Flurkey, W.H. and Jen, J.J., 1978. Peroxidase and polyphenol oxidase activities in developing peaches. Journal of Food Science, 43(6), 1826-1828.

Zhang, D.Q., Pang, X., Xuewu, D., Ji, Z. and Jiang, Y.M., 2005. Role of peroxidation in anthocyanin degradation in litchi fruit pericarp. Food Chemistry, 90, 47-52.

Benzie, I.F.F. and Strain, J.J., 1999. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15-27.

Brand-Williams, W., Cuvelier, M. E. and Berset, C., 1995. Use of free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft and Technologie, 28, 25-30.

Ukeda, H., Maeda, S., Ishii, T. and Sawamura, M., 1997. Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3′-{1-[(phenylamino)-carbonyl]-3,4-tetrazolium}-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate reduction by xanthine–xanthine oxidase. Analytical Biochemistry, 251(2), 206-209.

Aebi, H., 1984. Catalase in vitro. Method in Enzymology, 105, 121-126.

Cuvi, M.J., Vicente, A.R., Concellón, A. and Chaves, A.R., 2011. Changes in red pepper antioxidants as affected by UV-C treatments and storage at chilling temperatures. LWT - Food Science and Technology, 44(7), 1666-1671.

Boonyaritthongchai, P., Supapvanich, S., Wongaree, C., Uthairatanakij, A., Jitareerat, P., Pongprasert, N. and Kaewmanee, N., 2018. Application of natural extracts from pineapple juice on inhibiting browning symptom of fresh-cut “Nam dok mai” mango. Acta Horticulturae, 1210, 235-240, https://doi.org/10.17660/ActaHortic.2018.1210.33.

Supapvanich, S., Mitrsang, P. and Srinorkham, P., 2017. Effects of ‘Queen’ and ‘Smooth cayenne’ pineapple fruit core extracts on browning inhibition of fresh-cut wax apple fruit during storage. International Food Research Journal, 24(2), 559-564.

Rux, G., Caleb, O., Ulrichs, C., Huyskens-Keil, S., Herppich, W.B. and Mahajan, P., 2017. Impact of Dipping Fresh Cut Apple Slices in Different Sugar Solutions on Quality Parameters. [Online] Available at: https://www.researchgate.net/profile/Guido-Rux/publication/330798104_Impact_of_fresh-cut_apple_slices_in_different_sugar_solutions_on_quality_parameters/links/5c545ebf92851c22a3a12a65/Impact-of-dipping-fresh-cut-apple-slices-in-different-sugar-solutions-on-quality-parameters.pdf.

García-García, R. and Searle, S.S., 2015. Preservatives: Food use. In: B. Caballero, P. Finglas and F. Toldra, eds. Encyclopedia of Food and Health. Cambridge: Academic Press, pp. 505-509.

Puangphet, A., Tiyaboonchai, W. and Thongsook, T., 2015. Inhibitory effect of sericin hydrolysate on polyphenol oxidase and browning of fresh-cut products. International Food Research Journal, 22(4), 1623-1630.

Thongsook, T. and Tiyaboonchai, W., 2011. Inhibitory effect of sericin on polyphenol oxidase and its application as edible coating. Journal of Food Science and Technology, 46, 2052-2061.

Takeungwongtrakul, S., Sai-Ut, S., Waraput, T. and Supapvanich, S., 2022. Thermal processes improving antibrowning potential of mixed Aloe vera and pineapple core extract solution on browning inhibition of fresh-cut apples. International Journal of Food Science and Technology, 57(10), 6881-6889.

Beaulieu, M., Beliveau, G.M. and Lacroix, M., 1999. Does rate effect of irradiation on the phenolic compounds, polyphenol oxidase and browning of mushrooms (Agaricus bisporous). Journal of Agricultural and Food Chemistry, 47(7), 2537-2543.

Murata, M., Tsurutani, M., Tomita, M., Homma, S. and Kaneko, K., 1995. Relationship between apple ripening and browning: Changes in polyphenol content and polyphenol oxidase. Journal of Agricultural and Food Chemistry, 43, 1115-1121.

Singh, B., Suri, K., Shevkani, K., Kaur, A., Kaur, A. and Singh, N., 2018. Enzymatic browning of fruit and vegetables: A review. In: M. Kuddus, ed., Enzymes in Food Technology. Singapore: Springer, pp. 63-78.

Zhang, X. and Shao, X., 2015. Characterisation of polyphenol oxidase and oeroxidase and the role in browning of loquat fruit. Czech Journal of Food Sciences, 33(2), 109-117.

Chisari, M., Barbagallo, R.N. and Spagna, G., 2007. Characterization and role of polyphenol oxidase and peroxidase in browning of fresh cut melon. Journal of Agricultural and Food Chemistry, 56, 132-138.

Kyung-Mi, J., In-Gyu, S., Se-Jong, K. and Sang-Han, L., 2015. Effect of polyphenol oxidase activity and total phenolic content on browning and quality of dried-persimmon according to maturity degree of astringent persimmon. Current Research on Agriculture and Life Science, 33(2), 65-68.

Wu, J.H., Wang, Z. and Xu, S.Y., 2007. Preparation and characterization of sericin powder extracted from silk industry wastewater. Food Chemistry, 103, 1255-1262.

Sudheesh, C. and Sunooj, K.V., 2021. Cold plasma processing of fresh-cut fruits and vegetables. In: M.W. Siddiqui, ed. Fresh-Cut Fruits and Vegetables. Cambridge: Academic Press, pp. 339-356.

Lindley, M.G. 1998. The impact of food processing on antioxidants in vegetable oils, fruits and vegetables. Trends in Food Science and Technology, 9, 336-340.

Micheal, A.S. and Subramanyam, M., 2014. Influence of sericin in alleviating the hydrogen peroxide induced oxidative stress in silkworm Bombyx mori: Role of the amino acids. Information Systems Journal, 11, 257-272.

Saptarini, N.M., Rahayu, D. and Herawati, I.E., 2019. Antioxidant activity of crude bromelain of pineapple (Ananas comosus (L.) Merr) crown from Subang district, Indonesia. Journal of Pharmacy and Bioallied Sciences, 11(Suppl. 4), S551-S555.

Borgstahl, G.E.O. and Oberley-Deegan, R.E., 2018. Superoxide dismutases (SODs) and SOD mimetics. Antioxidants, 7(11), https://doi.org/10.3390/antiox7110156.