Impact of Phosphorus and Rhizobium on Mungbean Seed Yield and Nodulation

Main Article Content

Joysree Pual Tandra
Papon Chandra Paul
Md. Rasheduzzaman
Md. Rashedur Rahman
Md. Ekram ul Haque

Abstract

Mungbean plays a crucial role as a rotational crop, enhancing soil fertility and contributing to sustainable nutrient management. The integration of biofertilizers further supports environmentally friendly and sustainable farming practices. To investigate the combined influence of Rhizobium inoculation and phosphorus fertilization on the yield of mungbean (cv. Binamoog-8), an experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, from October 2023 to February 2024. The study was designed as a split-plot with three replications. Rhizobium inoculation (two levels: without inoculation and with inoculation) was assigned to the main plots, while phosphorus levels (five levels: 0, 10, 15, 20, and 25 kg P ha-1) were allocated to the subplots. Seeds were pre-treated with molasses and subsequently coated with microbial (Rhizobium) fertilizer before sowing. The findings showed that the application of Rhizobium inoculation and 20 kg of phosphorus ha-1 enhanced the growth parameters (plant height, number of branches plant-1 at 60 DAS and number of nodules plant-1 at 45 DAS) and the yield  parameters of mungbean (number of seeds pod-1, pod length, 1000 seeds weight, seed yield, stover yield, biological yield, and harvest index). The results of interaction effect revealed that the combined application of Rhizobium inoculation and 20 kg of phosphorus ha-1 improved the growth parameters (plant height, number of branches plant-1 at 60 DAS and number of nodules plant-1 at 45 DAS) and the yield parameters of the crop (pod length, 1000 seeds weight, seed yield). The soil quality was also improved by the combined application of Rhizobium and phosphorus. The result showed an effective improvement of soil pH, organic matter, soil nitrogen, sulfur, potassium, and phosphorus from the combination of Rhizobium inoculation and 20 kg of phosphorus ha-1 application. Thus, the use of Rhizobium inoculation and 20 kg of phosphorus ha-1 in mungbean cultivation is recommended in order to obtain the maximum seed output.

Article Details

How to Cite
Pual Tandra, J. ., Chandra Paul, P. ., Rasheduzzaman, M., Md. Rashedur Rahman, & Md. Ekram ul Haque. (2026). Impact of Phosphorus and Rhizobium on Mungbean Seed Yield and Nodulation. CURRENT APPLIED SCIENCE AND TECHNOLOGY, e0267431. https://doi.org/10.55003/cast.2026.267431
Section
Original Research Articles

References

Ahammad, T., Islam, M. Z., Islam, M. A., Habib, M., Hossain, M. A., & Khan, M. J. I. (2025). Effects of zinc fertilization on various Mungbean cultivars in the Madhupur tract of Bangladesh. Journal of Bangladesh Agricultural University, 23(1), 9-17. http://dx.doi.org/10.3329/jbau.v23i1.80818

Alene, A. A., Raffi, M. M., & Tiruneh, K. J. (2021). Phosphorus use efficiency, yield and nodulation of mung bean (Vigna radiata L.) as influenced by the rate of phosphorus and Rhizobium strains inoculation in Metema district, Ethiopia. Journal of Plant Nutrition, 44(9), 1300-1315. https://doi.org/10.1080/01904167.2020.1849301

Alori, E. T., Glick, B. R., & Babalola, O. O. (2017). Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Frontiers in Microbiology, 8, Article 971. https://doi.org/10.3389/fmicb.2017.00971

Arif, H., Amjed, A., Tasneem, K., Ashfaq, A., Zubair, A., & Muhammad, A. (2014). Growth, nodulation and yield components of mung bean (Vigna radiata) as affected by phosphorus in combination with rhizobium inoculation. African Journal of Agricultural Research, 9(30), 2319-2323. https://doi.org/10.5897/AJAR11.691

Bam, R., Mishra, S. R., Khanal, S., Ghimire, P., & Bhattarai, S. (2022). Effect of biofertilizers and nutrient sources on the performance of mungbean at Rupandehi, Nepal. Journal of Agriculture and Food Research, 10, Article 100404. https://doi.org/10.1016/j.jafr.2022.100404

BBS. (2023). Yearbook of agricultural statistics. Bangladesh Bureau of Statistics, Statistics and Informatics Division, Ministry of Planning, Government of the People’s Republic of Bangladesh.

Beovides-García, Y., Pérez-Peñaranda2, M. C., González-Marrero, I., Rayas-Cabrera, A., Basail-Pérez, M., Santos-Pino, A., Medero-Vega, V. R., & Mok, M. J. A. (2024). Effects of a new Rhizobium-based biofertilizer (Fertiriz) on growth and the yield of Cowpea (Vigna unguiculata (L.) Walp). Environmental Analysis and Ecology Studies, 12(1), Article 000778. https://doi.org/10.31031/EAES.2024.12.000778

Das, S. K. (2016). Effect of phosphorus and sulphur on yield attributes, yield, nodulation and nutrient uptake of green gram [Vigna radiata (L.) Wilczek]. Legume Research, 40(1), 138-143.

Dey, S. K., Singh, V., & Tiwari, D. 2021. Effect of phosphorus and sulphur on the growth and yield of summer mungbean (Vigna radiata L.). The Pharma Innovation, 10(8), 1442-1445.

Dissanayaka, D. M. S. B., Ghahremani, M., Siebers, M., Wasaki, J., & Plaxton, W. C. (2021). Recent insights into the metabolic adaptations of phosphorus-deprived plants. Journal of Experimental Botany, 72(2), 199-223. https://doi.org/10.1093/jxb/eraa482

Dubey, S. N., Singh, R., Kumar, R., & Dubey, S. (2018). Effect of phosphorus and PSB on growth, nodulation and fertility status in different mungbean (Vigna radiata L.) varieties and its residual effect on fodder yield of sorghum in indo-gangetic plain zone of India. International Journal of Agricultural Sciences, 14(1), 196-201. https://doi.org/10.15740/HAS/IJAS/14.1/196-201

El-Badawy, M. El. M., & Mehasen, S. A. S. (2012). Correlation and path coefficient analysis for yield and yield components of soybean genotypes under different planting density. Asian Journal of Crop Science, 4(4), 150-158. https://doi.org/10.3923/ajcs.2012.150.158

Gebremariam, M., & Tesfay, T. (2021). Effect of P application rate and Rhizobium inoculation on nodulation, growth, and yield performance of chickpea (Cicer arietinum L.). International Journal of Agronomy, 2021(1), Article 8845489. https://doi.org/10.1155/2021/8845489

Geletu, T., & Mekonnen, F. (2018). The effect of bio and inorganic fertilizer on yield, nutrient uptake and economics of mungbean (Vigna radiata L. Wilczek) varieties in Ethiopia. Journal of Agriculture and Research, 3(11), Article 00212. https://doi.org/10.23880/oajar-16000212

Ghafoor, A., Mahmood, K., Najeeb, S., Hussain, S., & Bilal, M. (2023). Influence of Rhizobium and phosphorus on growth, yield and quality of mungbean (Vigna radiata L.) under arid conditions. Journal of Animal and Plant Sciences, 33(1), 107-117.

Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research (2nd ed.). John Wiley and Sons.

Gough, E. C., Owen, K. J., Zwart, R. S., & Thompson, J. P. (2021). Arbuscular mycorrhizal fungi acted synergistically with Bradyrhizobium sp. to improve nodulation, nitrogen fixation, plant growth and seed yield of mung bean (Vigna radiata) but increased the population density of the root-lesion nematode Pratylenchus thornei. Plant and Soil, 465(1-2), 431-452. https://doi.org/10.1007/s11104-021-05007-7

Hannan, A., Huda, M. S., Sultana, M., Alam, M. R., & Islam, M. A. (2022). Effects of different levels of phosphorus and bradyrhizobium inocula on the productivity and protein content of mungbean (Vigna radiata L. Wilczek). Open Access Journal of Science, 5(1), 32-39. https://doi.org/10.15406/oajs.2022.05.00173

Hasan, M., Sultana, S., & Jahan, M. A. (2023). The growth and yield performances of mungbean (Vigna radiata L.) as influenced by the use of cow dung and chemical fertilizers. Journal of Bangladesh Academy of Sciences, 47(1), 61-68. https://doi.org/10.3329/jbas.v47i1.66025

Htwe, A. Z., Moh, S. M., Moe, K., & Yamakawa, T. (2018). Effects of co-inoculation of Bradyrhizobium japonicum SAY3-7 and Streptomyces griseoflavus P4 on plant growth, nodulation, nitrogen fixation, nutrient uptake, and yield of soybean in a field condition. Soil Science and Plant Nutrition, 64(2), 222-229. https://doi.org/10.1080/00380768.2017.1421436

Htwe, A. Z., Moh, S. M., Soe, K. M., Moe, K., & Yamakawa, T. (2019). Effects of biofertilizer produced from Bradyrhizobium and Streptomyces griseoflavus on plant growth, nodulation, nitrogen fixation, nutrient uptake, and seed yield of mungbean, cowpea, and soybean. Agronomy, 9(2), Article 77. https://doi.org/10.3390/agronomy9020077

Hussain, A., Ali, A., Khaliq, T., Ahmad, A., Aslam, Z., & Asif, M. (2014). Growth, nodulation and yield components of mung bean (Vigna radiata) as affected by phosphorus in combination with Rhizobium inoculation. African Journal of Agricultural Research, 9(30), 2319-2323. https://doi.org/10.5897/AJAR11.691

Ikbal, Passricha, N., Saifi, S. K., Sikka, V. K., & Tuteja, N. (2020). Multilegume biofertilizer: a dream. In V. Sharma, R. Salwan, & L. K. T. Al-Ani (Eds.). Molecular aspects of plant beneficial microbes in agriculture (pp. 35-45). https://doi.org/10.1016/B978-0-12-818469-1.00003-1

Islam, M. K., Islam, S. M. A., Harun-or-Rashid, M., Hossain, A. F. M. G. F., & Alom, M. M. (2006). Effect of biofertilizer and plant growth regulators on growth of summer mungbean. International Journal of Botany, 2(1), 36-41.

Islam, M. R., Sarker, U., Azam, M. G., Hossain, J., Alam, M. A., Ullah, R., Bari, A., Hossain, N., Sabagh, A. E., & Islam, M. S. (2024). Potassium augments growth, yield, nutrient content, and drought tolerance in mung bean (Vigna radiata L. Wilczek.). Scientific Reports, 14, Article 9378. https://doi.org/10.1038/s41598-024-60129-z

Jamir, A., Sentimenla, & Gohain, T. (2022). Response of phosphorus and biofertilizers on growth, yield attributes and economic indices of black gram (Vigna mungo L. Hepper). International Journal of Environment and Climate Change, 12(11), 3793-3801. https://doi.org/10.9734/ijecc/2022/v12i111432

Kumar, A., Yadav, P., Seema, Kumar, R. R., Kushwaha, A., Rashid, M. H., Tarannum, N., & Chakraborty, S. (2022). Mungbean (Vigna radiata (L.) R. Wilczek): Progress in breeding and future challenges. International Journal of Plant and Soil Science, 34(3), 50-59. https://doi.org/10.9734/ijpss/2022/v34i330846

Kundu, N. D., Uddin, M. T., Sarkar, M. M. A., Toma, N. I., Rashid, M. M., & Ahmed, S. (2025). Profitability and value addition of mungbean production in Barishal and Patuakhali districts of Bangladesh. Bangladesh Journal of Nuclear Agriculture, 38(2), 137-149.

Li, H., Wang, L., Zhang, Z., Yang, A., & Liu, D. (2022). Effect of phosphorus supply levels on nodule nitrogen fixation and nitrogen accumulation in soybean (Glycine max L.). Agronomy, 12(11), Article 2802.

Ma, Y., & Chen, R. (2021). Nitrogen and phosphorus signaling and transport during legume-rhizobium symbiosis. Frontiers in Plant Science, 12, Article 683601. https://doi.org/10.3389/fpls.2021.683601

Ma, Y., Suo, Y., Qi, H., Tang, F., & Wang, M. (2024). Effects of Rhizobium inoculation on rhizosphere soil microbial communities, physicochemical properties, and enzyme activities in Caucasian clover under field conditions. Agronomy, 14(12), Article 2880. https://doi.org/10.3390/agronomy14122880

Magadlela, A., Pérez-Fernández, M. A., Kleinert, A., Dreyer, L. L., & Valentine, A. J. (2016). Source of inorganic N affects the cost of growth in a legume tree species (Virgilia divaricata) from the Mediterranean-type Fynbos ecosystem. Journal of Plant Ecology, 9(6), 752-761. https://doi.org/10.1093/jpe/rtw015

Magadlela, A., Vardien, W., Kleinert, A., Dreyer, L. L., & Valentine, A. J. (2015). The role of phosphorus deficiency in nodule microbial composition, and carbon and nitrogen nutrition of a native legume tree in the Cape Fynbos ecosystem. Australian Journal of Botany, 63(5), 379-386. https://doi.org/10.1071/BT14216

Matse, D. T., Huang, C. H., Huang, Y. M., & Yen, M. Y. (2020). Effects of coinoculation of Rhizobium with plant growth promoting rhizobacteria on the nitrogen fixation and nutrient uptake of Trifolium repens in low phosphorus soil. Journal of Plant Nutrition, 43(5), 739-752. https://doi.org/10.1080/01904167.2019.1702205

Mbaka, F. K., Ndukhu, H. O., Oloo-abucheli, G. O., & Kiplangat, C. T. (2022). Effect of Rhizobium inoculation and phosphate rock fertilizer application on biomass production, nutrient use efficiency and yield parameters of green gram (Vigna radiata). Asian Journal of Agricultural and Horticultural Research, 9(2), 47-59. https://doi.org/10.9734/ajahr/2022/v9i230141

Nair, R. M., Schafleitner, R., & Lee, S. H. (2020). The mungbean genome. Springer International Publishing. https://doi.org/10.1007/978-3-030-20008-4

Odoom, A., & Ofosu, W. (2024). Role of phosphorus in the photosynthetic dark phase biochemical pathways. In N. A. Anjum, A. Masood, S. Umar, & N. A. Khan (Eds.). Phosphorus in soils and plants (pp. 1-16). IntechOpen. https://doi.org/10.5772/intechopen.112573

Pérez-Fernández, M., Míguez-Montero, Á., & Valentine, A. (2019). Phosphorus and nitrogen modulate plant performance in shrubby legumes from the Iberian Peninsula. Plants, 8(9), Article 334. https://doi.org/10.3390/plants8090334

Rani, M., Prakash, V., Khan, K., (2016). Response of mungbean [Vigna radiata (L.) Wilczek] to phosphorus, sulphur and PSB during summer season. Agricultural Science Digest, 36(2), 146-148. https://doi.org/10.18805/asd.v36i2.10637

Seleiman, M. F., Almutairi, K. F., Alotaibi, M., Shami, A., Alhammad, B. A., & Battaglia, M. L. (2020). Nano-fertilization as an emerging fertilization technique: Why can modern agriculture benefit from its use? Plants, 10(1), Article 2. https://doi.org/10.3390/plants10010002

Singh, K., Manohar, R. S., Choudhary, R., Yadav, A. K., & Sangwan, A. (2015). Response of different sources and levels of phosphorus on yield, nutrient uptake and net returns on mungbean under rainfed condition. Agricultural Science Digest, 35(4), 263-268. https://doi.org/10.18805/asd.v35i4.6856

Soe, K., & Yamakawa, T. (2013). Low-density co-inoculation of Myanmar Bradyrhizobium yuanmingense MAS34 and Streptomyces griseoflavus P4 to enhance symbiosis and seed yield in soybean varieties. American Journal of Plant Sciences, 4(9), 1879-1892. https://doi.org/10.4236/ajps.2013.49231

Sood, A., Bochalya, R. S., Choudhary, K., Shubham, & Katoch, A. (2023). Effect of biofertilizer and fertility levels on growth characters of mungbean under mid hills of Himachal Pradesh, India. International Journal of Plant and Soil Science, 35(20), 1271-1277. https://doi.org/10.9734/ijpss/2023/v35i203926

Tena, W., Wolde-Meskel, E., & Walley, F. (2016). Symbiotic efficiency of native and exotic Rhizobium strains nodulating lentil (Lens culinaris Medik.) in soils of Southern Ethiopia. Agronomy, 6(1), Article 11. https://doi.org/10.3390/agronomy6010011

Terefe, M., Shifa, K., Tilahum, A., Ibrahim, A., Biratu, K., & Bogale, A. (2018). Performance of different mulberry/Morus sp./genotypes and their effect on mulberry silkworm, Bombyx mori (Lepidoptera: Bombycidae). Journal of Agriculture and Research, 3(11), Article 000210. https://doi.org/10.23880/oajar-16000210

Venkatarao, C. V., Naga, S. R., Yadav, B. L., Koli, D. K., & Rao, I. J. (2017). Effect of phosphorus and biofertilizers on growth and yield of mungbean [Vigna radiata (L.) Wilczek]. International Journal of Current Microbiology and Applied Sciences, 6(7), 3992-3997. https://doi.org/10.20546/ijcmas.2017.607.413

Yao, Y., Wu, D., Gong, Z., Zhao, J., & Ma, C. (2018). Variation of nitrogen accumulation and yield in response to phosphorus nutrition of soybean (Glycine max L. Merr.). Journal of Plant Nutrition, 41(9), 1138-1147. https://doi.org/10.1080/01904167.2018.1433834