Seed Coating with Fungicidal Agents: Enhancing Quality, Storability, and Fusarium sp. Inhibition in Vegetable Soybean Seeds

Main Article Content

Davika Rapeebunyanon
Chatsuda Phuakjaiphaeo
Vassana Viroonrat
Jakkrapong Kangsopa

Abstract

This study evaluated the effects of fungicidal seed coating on Fusarium sp. inhibition, seed quality, and seedling growth in vegetable soybeans. Laboratory tests showed that mancozeb, prochloraz, and carboxin completely inhibited fungal growth for nine days, whereas thiram, captan, and metalaxyl-M provided only partial suppression. Among the tested fungicides, prochloraz was most effective in maintaining seed germination and seedling vigor. In seed coating trials, prochloraz applied at 6 g of active ingredient (g.ai.) increased shoot length to 26.5 cm and root length to 21.3 cm under sand test conditions, while mancozeb and carboxin produced similar but slightly lower values. Seedling survival reached up to 87% with prochloraz at 4 to 6 g.ai., compared with only 65% in uncoated seeds. Storage experiments demonstrated that prochloraz-coated seeds maintained germination above 85% for up to 4 months under controlled conditions, whereas uncoated seeds dropped below 70%. Under ambient conditions, germination of all treatments declined after 4 months, but coated seeds still performed better than untreated ones. Overall, prochloraz at 6 g.ai. was identified as the most effective treatment for improving germination, seedling vigor, and disease resistance, particularly in the short-term storage of vegetable soybean seeds. These findings highlight the practical value of fungicidal seed coating as a cost-effective strategy for protecting soybean seeds from early infection and improving seedling establishment in pathogen-prone environments.

Article Details

How to Cite
Rapeebunyanon, D., Phuakjaiphaeo, C., Viroonrat, V., & Kangsopa, J. (2026). Seed Coating with Fungicidal Agents: Enhancing Quality, Storability, and Fusarium sp. Inhibition in Vegetable Soybean Seeds . CURRENT APPLIED SCIENCE AND TECHNOLOGY, e0268666. https://doi.org/10.55003/cast.2025.268666
Section
Original Research Articles

References

Allen, T.W., Enebak, S.A. & Carey, W.A. (2004). Evaluation of fungicides for control of species of Fusarium on longleaf pine seed. Crop Protection, 23(10), 979-982.

AOSA. (1983). Seed vigor testing handbook. Association of Official Seed Analysis.

Bewley, J. D., & Black, M. (2013). Seeds: physiology of development and germination. Springer Science & Business Media.

Blanco, R., & Aveling, T. A. S. (2016). Seed-borne Fusarium pathogens in agricultural crops. Acta Horticulturae, 1204, 161-170. https://doi.org/10.17660/ActaHortic.2018.1204.21

Buttar, H. S., Singh, A., Sirari, A., Anupam, Kaur, K., Kumar, A., Lal, M. K., & Kumar, R. (2023). Investigating the impact of fungicides and mungbean genotypes on the management of pod rot disease caused by Fusarium equiseti and Fusarium chlamydosporum. Frontiers in Plant Science, 14, Article 1164245. https://doi.org/10.3389/fpls.2023.1164245

Capo, L., Zappino, A., Reyneri, A., & Blandino, M. (2020). Role of the fungicide seed dressing in controlling seed-borne Fusarium spp. infection and in enhancing the early development and grain yield of maize. Agronomy, 10(6), Article 784. https://doi.org/10.3390/agronomy10060784

Chuenchan, W., Raksasanoy, S., Yooboriboon, S. & Kitja, W. (2019). Inhibition of Phytophthora parasitica by antagonistic molds from soil’s Kuiburi Subdistrict, Prachuap Khiri Khan Province. Journal of Science Ladkrabang, 28(1), 52-64.

Corbineau, F. (2024). The effects of storage conditions on seed deterioration and ageing: How to improve seed longevity. Seeds, 3(1), 56-75.

Ellis, M. L., Broders, K. D., Paul, P. A., & Dorrance, A. E. (2011). Infection of soybean seed by Fusarium graminearum and effect of seed treatments on disease under controlled conditions. Plant Disease, 95(4), 401-407.

Ellis, R. H., & Roberts, E. H. (1980). Improved equations for the prediction of seed longevity. Annals of botany, 45(1), 13-30.

Ergin, N., Kulan, E. G., & Kaya, M. D. (2021). The effects of fungicidal seed treatments on seed germination, mean germination time and seedling growth in safflower (Carthamus tinctorius L.). Selcuk Journal of Agriculture and Food Sciences, 35(2), 139-143.

Gorim, L., & Asch, F. (2017). Seed coating increases seed moisture uptake and restricts embryonic oxygen availability in germinating cereal seeds. Biology, 6(2), Article 31. https://doi.org/10.3390/biology6020031

Gubišová, M., Hudcovicová, M., Hrdlicová, M., Ondreičková, K., Cilík, P., Klčová, L., Kaňuková, Š. and Gubiš, J., (2024). Superabsorbent seed coating and its impact on fungicide efficacy in a combined treatment of barley seeds. Agriculture, 14(5), Article 707. https://doi.org/10.3390/agriculture14050707

Harish, J., Jambhulkar, P. P., Bajpai, R., Arya, M., Babele, P. K., Chaturvedi, S. K., Kumar, A. & Lakshman, D. K. (2023). Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize. Frontiers in Microbiology, 14, Article 1121781. https://doi.org/10.3389/fmicb.2023.1121781

Halmer, P. (2008). Seed technology and seed enhancement. Acta Horticulturae, 771, 17-26. https://doi.org/10.17660/ActaHortic.2008.771.1

Ibrahim, E. A. M. (2015). Effect of some treatments on seed health and viability of soybean. Plant Pathology Journal, 14(4), 158-167.

ISTA. (2023). International rules for seed testing. International Seed Testing Association.

Kangsopa, J., & Atnaseo, C. (2022). Seed coating application of endophytic and rhizosphere bacteria for germination enhancement and seedling growth promotion in soybeans. International Journal of Agricultural Technology, 18(1), 215-230.

Kangsopa, J., Singsopa, A., Thawong, N., Baomeesri, S., Rapeebunyanon, D., & Charoenyai, S. (2024). Effects of seed pelleting with fungicide on seed quality and inhibition of Fusarium sp. in Chili (Capsicum annuum L.). Songklanakarin Journal of Science and Technology, 46(5), 430-437.

Kangsopa, J., Singsopa, A., & Thawong, N. (2023). Effects of different binder types and concentrations on physical and quality properties in marigold (Tagetes erecta L.) seed pelleting. Songklanakarin Journal of Science and Technology, 45(4), 494-500.

Koohakan, P., Prasom, P., & Sikhao, P. (2020). Application of seed coating with endophytic bacteria for Fusarium wilt disease reduction and growth promotion in tomato. International Journal of Agricultural Technology, 16(1), 55-62.

Kunwanlee, P., Maneerat, T. & Plodjinda, K. (2023). Effect of tomato seed priming with bacillus subtilis on seed germination, and seedling survival in outbreak bacterial wilt in greenhouse condition. VRU Agricultural and Food Journal, 2(1), 22-28.

Liu, J., Cui, W., Zhao, Q., Ren, Z., Li, L., Li, Y., Sun., L. & Ding, J. (2025). Identification, characterization, and chemical management of Fusarium asiaticum causing soybean root rot in Northeast China. Agronomy, 15(2), Article 388. https://doi.org/10.3390/agronomy15020388

Ma, G. H., Duan, X. M., & Xu, W. H. (2022). Identification and laboratory screening of chemical agents of root rot pathogens of Astragalus membranaceus var. mongholicus. Acta Agrestia Sinica, 30, 1122-1130.

Mancini, V., & Romanazzi, G. (2014). Seed treatments to control seedborne fungal pathogens of vegetable crops. Pest Management Science, 70(6), 860-868.

Nagashima, S., Tsukamoto, T., Isota, J., Kako, T., & Tojo, M. (2020). Control effects of metalaxyl-M and azoxystrobin on stem and root rot pathogens of Hydrangea macrophylla. Annual Report of the Kansai Plant Protection Society, 62, 153-156. https://doi.org/10.4165/kapps.62.153

Nair, R. M., Boddepalli, V. N., Yan, M.-R., Kumar, V., Gill, B., Pan, R. S., Wang, C., Hartman, G. L., Souza, R. S. E., & Somta, P. (2023). Global status of vegetable soybean. Plants, 12(3), Article 609. https://doi.org/10.3390/plants12030609

Norkaew, J., Khemmuk, W., McGovern, J. & To-anun, C. (2021). Selection of antagonistic bacteria against Fusarium fujikuroi causing bakanae disease of rice. Khon Kaen Agriculture Journal, 49(1), 144-154.

Panth, M., Hassler, S. C., & Baysal-Gurel, F. (2020). Methods for management of soilborne diseases in crop production. Agriculture, 10(1), Article 16. https://doi.org/10.3390/agriculture10010016

Paravar, A., Piri, R., Balouchi, H., & Ma, Y. (2023). Microbial seed coating: An attractive tool for sustainable agriculture. Biotechnology Reports, 37, Article e00781. https://doi.org/10.1016/j.btre.2023.e00781

Paulitz, T. C., & Bélanger, R. R. (2001). Biological control in greenhouse systems. Annual Review of Phytopathology, 39(1), 103-133.

Ramtekey, V., Cherukuri, S., Kumar, S., V., S. K., Sheoran, S., K., U. B., K., B. N., Kumar, S., Singh, A. N., & Singh, H. V. (2022). Seed longevity in legumes: deeper insights into mechanisms and molecular perspectives. Frontiers in Plant Science, 13, Article 918206. https://doi.org/10.3389/fpls.2022.918206

Rétif, F., Kunz, C., Calabro, K., Duval, C., Prado, S., Bailly, C., & Baudouin, E. (2023). Seed fungal endophytes as biostimulants and biocontrol agents to improve seed performance. Frontiers in Plant Science, 14, Article 1260292. https://doi.org/10.3389/fpls.2023.1260292

Rogério, F., Silva, T. D., Santos, J. D., Migliavacca, R. A., Cazado, J. F., Arieira, C. R. D., Salvestro, A. D. C., Oliveira, V. D. & Lima, W. S., (2012). Seed treatment influence with carboxin+ thiram to initial development of safflower plants. Journal of Food, Agriculture and Environment, 10, 675-676.

Rose, S., Parker, M., & Punja, Z. K. (2003). Efficacy of biological and chemical treatments for control of Fusarium root and stem rot on greenhouse cucumber. Plant disease, 87(12), 1462-1470.

Rupe, J. C. (1992). Nature and management of soilborne fungal diseases of soybean. In L. G. Copping, M. B. Green, & R. T. Rees (Eds.). Pest management in soybean (pp. 196-205). Springer. https://doi.org/10.1007/978-94-011-2870-4_19

Shen, G., Teng, H., Sun, J., Xu, X., Jiao, C., Fan, X., & Zhao, J. (2024). Baseline sensitivity and toxicity mechanisms of prochloraz to Alternaria alternata strains associated with maize leaf blight in Heilongjiang province in China. Plant Disease, 108(11), 3336-3344.

Walters, C., Ballesteros, D., & Vertucci, V. A. (2010). Structural mechanics of seed deterioration: standing the test of time. Plant Science, 179(6), 565-573.

Wolny, E., Betekhtin, A., Rojek, M., Braszewska-Zalewska, A., Lusinska, J., & Hasterok, R. (2018). Germination and the early stages of seedling development in Brachypodium distachyon. International Journal of Molecular Sciences, 19(10), Article 2916. https://doi.org/10.3390/ijms19102916

Xing, W., Li, Y., Zhou, L., Hong, H., Liu, Y., Luo, S., Zou, J., Zhao, Y., Yang, Y., Xu, Z. & Tan, B., (2025). Deciphering seed deterioration: Molecular insights and priming strategies for revitalizing aged seeds. Plants, 14(11), Article 1730. https://doi.org/10.3390/plants14111730

Zaim, N. S. H. B. H., Tan, H. L., Rahman, S. M. A., Bakar, N. F. A, Osman, M. S., Thakur, V. K., & Radacsi, N. (2023). Recent advances in seed coating treatment using nanoparticles and nanofibers for enhanced seed germination and protection. Journal of Plant Growth Regulation, 42(12), 7374-7402. https://doi.org/10.1007/s00344-023-11038-4

Zakaria, L. (2023). Fusarium species associated with diseases of major tropical fruit crops. Horticulturae, 9(3), Article 322. https://doi.org/10.3390/horticulturae9030322