Phytochemical Study of Orchidaceae: Composition, Bioactivity and Potential Application in Functional Foods

Main Article Content

Syntiya Inanda Khoidir
Deksa Aldebaran
Nur Aniza Aprilia
Dimas Setyadi Putra

Abstract

 


The family Orchidaceae is one of the most taxonomically diverse groups of angiosperms, recognized for their ornamental significance and their content of pharmacologically relevant secondary metabolites. This review critically evaluates the phytochemical diversity, bioactivities, and potential of Orchidaceae species as natural sources for the development of functional food products. This review is based on a comprehensive literature survey of 32 peer-reviewed articles indexed in Scopus and PubMed through April 2025, which reports the presence of diverse bioactive compounds-mainly phenolics, flavonoids, alkaloids, tannins, and anthocyanins in various orchid species and plant tissues. These compounds exhibit a broad spectrum of biological activities, including antioxidants, antimicrobial, anticancer, anti-inflammatory, antidiabetic, anti-aging, photoprotective, and neuropharmacological effects. The concentration and composition of these metabolites depend on species differences, plant organ specificity, and extraction methods. Cumulative evidence underscores the underutilized potential of orchids as phytochemical reservoirs for nutraceutical applications. However, further translational research on bioavailability, safety, and functional stability in complex food systems is essential to enable their practical application in consumer health products. This review advocates intensive multidisciplinary research on Orchidaceae to open up new opportunities in the functional food and health-promoting bioactive industries.

Article Details

How to Cite
Khoidir, S. I., Aldebaran, D., Aprilia, N. A., & Putra, D. S. (2026). Phytochemical Study of Orchidaceae: Composition, Bioactivity and Potential Application in Functional Foods. CURRENT APPLIED SCIENCE AND TECHNOLOGY, e0269318. https://doi.org/10.55003/cast.2026.269318
Section
Review Ariticle

References

Athipornchai, A., & Jullapo, N. (2018). Tyrosinase inhibitory and antioxidant activities of orchid (Dendrobium spp.). South African Journal of Botany, 119, 188-192. https://doi.org/10.1016/j.sajb.2018.09.003

Barragán-Zarate, G. S., Lagunez-Rivera, L., Solano, R., Carranza-Álvarez, C., Hernández-Benavides, D. M., & Vilarem, G. (2022). Validation of the traditional medicinal use of a Mexican endemic orchid (Prosthechea karwinskii) through UPLC-ESI-qTOF-MS/MS characterization of its bioactive compounds. Heliyon, 8(7), Article e09867. https://doi.org/10.1016/j.heliyon.2022.e09867

Bazzicalupo, M., Burlando, B., Denaro, M., Barreca, D., Trombetta, D., Smeriglio, A., & Cornara, L. (2019). Polyphenol characterization and skin-preserving properties of hydroalcoholic flower extract from Himantoglossum robertianum (Orchidaceae). Plants, 8(11), Article 502. https://doi.org/10.3390/plants8110502

Bazzicalupo, M., Calevo, J., Smeriglio, A., & Cornara, L. (2023). Traditional, therapeutic uses and phytochemistry of terrestrial European orchids and implications for conservation. Plants, 12(2), Article 257. https://doi.org/10.3390/plants12020257

Bhatnagar, M., Sarkar, N., Gandharv, N., Apang, O., Singh, S., & Ghosal, S. (2017). Evaluation of antimycobacterial, leishmanicidal and antibacterial activity of three medicinal orchids of Arunachal Pradesh, India. BMC Complementary and Alternative Medicine, 17(1), Article 379. https://doi.org/10.1186/s12906-017-1884-z

Bhattacharyya, P., Kumaria, S., Job, N., & Tandon, P. (2015). Phyto-molecular profiling and assessment of antioxidant activity within micropropagated plants of Dendrobium thyrsiflorum: a threatened, medicinal orchid. Plant Cell, Tissue and Organ Culture, 122(3), 535-550. https://doi.org/10.1007/s11240-015-0783-6

Bose, B., Choudhury, H., Tandon, P., & Kumaria, S. (2017). Studies on secondary metabolite profiling, anti-inflammatory potential, in vitro photoprotective and skin-aging related enzyme inhibitory activities of Malaxis acuminata, a threatened orchid of nutraceutical importance. Journal of Photochemistry and Photobiology B: Biology, 173, 686-695. https://doi.org/10.1016/j.jphotobiol.2017.07.010

Casciaro, B., Calcaterra, A., Cappiello, F., Mori, M., Loffredo, M. R., Ghirga, F., Mangoni, M. L., Botta, B., & Quaglio, D. (2019). Nigritanine as a new potential antimicrobial alkaloid for the treatment of Staphylococcus aureus-induced infections. Toxins, 11(9), Article 511. https://doi.org/10.3390/toxins11090511

De, L. (2022). Chemical ingredients of important 75 medicinal orchids. International Journal of Chemistry Studies, 6(1), 73-79.

Essa, M. M., Bishir, M., Bhat, A., Chidambaram, S. B., Al-Balushi, B., Hamdan, H., Govindarajan, N., Freidland, R. P., & Qoronfleh, M. W. (2023). Functional foods and their impact on health. Journal of Food Science and Technology, 60(3), 820-834. https://doi.org/10.1007/s13197-021-05193-3

Eva, T. A., Mamurat, H., Rahat, M. H. H., & Hossen, S. M. M. (2024). Unveiling the pharmacological potential of Coelogyne suaveolens: An investigation of its diverse pharmacological activities by in vivo and computational studies. Food Science and Nutrition, 12(3), 1749-1767. https://doi.org/10.1002/fsn3.3867

Gantait, S., Das, A., Mitra, M., & Chen, J.-T. (2021). Secondary metabolites in orchids: Biosynthesis, medicinal uses, and biotechnology. South African Journal of Botany, 139, 338-351. https://doi.org/10.1016/j.sajb.2021.03.015

Hossen, S. M. M., Eva, T. A., Karim, M. S., Mamurat, H., Rahat, M. H. H., & Nipun, T. S. (2024). Antimicrobial potential, GCMS analysis and molecular docking studies of Coelogyne suaveolens extracts: Identification of bioactive compounds with mechanism of action. Biochemistry and Biophysics Reports, 37, Article 101648. https://doi.org/10.1016/j.bbrep.2024.101648

Hu, Y., Zhang, C., Zhao, X., Wang, Y., Feng, D., Zhang, M., & Xie, H. (2016). (±)-Homocrepidine A, a pair of anti-inflammatory enantiomeric octahydroindolizine alkaloid dimers from Dendrobium crepidatum. Journal of Natural Products, 79(1), 252-256. https://doi.org/10.1021/acs.jnatprod.5b00801

Kanlayavattanakul, M., Lourith, N., & Chaikul, P. (2018). Biological activity and phytochemical profiles of Dendrobium: A new source for specialty cosmetic materials. Industrial Crops and Products, 120, 61-70. https://doi.org/10.1016/j.indcrop.2018.04.059

Khan, H., Marya, Belwal, T., Tariq, M., Atanasov, A. G., & Devkota, H. P. (2019). Genus Vanda: A review on traditional uses, bioactive chemical constituents and pharmacological activities. Journal of Ethnopharmacology, 229, 46-53. https://doi.org/10.1016/j.jep.2018.09.031

Khoo, H. E., Azlan, A., Tang, S. T., & Lim, S. M. (2017). Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food and Nutrition Research, 61, Article 1361779. https://doi.org/10.1080/16546628.2017.1361779

Kiziltas, H., Goren, A. C., Alwasel, S. H., & Gulcin, İ. (2022). Sahlep (Dactylorhiza osmanica): Phytochemical analyses by LC-HRMS, molecular docking, antioxidant activity, and enzyme inhibition profiles. Molecules, 27(20), Article 6907. https://doi.org/10.3390/molecules27206907

Kotiloğlu, D., Acet, T., & Özcan, K. (2020). Phytochemical profile and biological activity of a therapeutic orchid from Anatolia: Dactylorhiza romana subsp. georgica. Journal of Food Measurement and Characterization, 14(6), 3310-3318. https://doi.org/10.1007/s11694-020-00566-2

Kumar, A., Mahanty, B., Goswami, R. C. D., Barooah, P. K., & Choudhury, B. (2021). In vitro antidiabetic, antioxidant activities and GC–MS analysis of Rhynchostylis Retusa and Euphorbia Neriifolia leaf extracts. 3 Biotech, 11(7), Article 315. https://doi.org/10.1007/s13205-021-02869-7

Kumari, P., Ujala, & Bhargava, B. (2021). Phytochemicals from edible flowers: Opening a new arena for healthy lifestyle. Journal of Functional Foods, 78, Article 104375. https://doi.org/10.1016/j.jff.2021.104375

Ma, J., Deng, Y., Wang, Y., Liu, Q., An, J., Li, M., Song, N., Zhang, J., Cheng, L., & Ma, K. (2021). A comparative study on ingredient and efficiency difference between fresh and steamed Gastrodia elata Blume: An herbal material to a novel functional food. Journal of Functional Foods, 82, Article 104512. https://doi.org/10.1016/j.jff.2021.104512

Mahomoodally, M. F., Picot-Allain, M. C. N., Zengin, G., Llorent-Martínez, E. J., Abdullah, H. H., Ak, G., Senkardes, I., Chiavaroli, A., Menghini, L., Recinella, L., Brunetti, L., Leone, S., Orlando, G., & Ferrante, C. (2020). Phytochemical analysis, network pharmacology and in silico investigations on Anacamptis pyramidalis tuber extracts. Molecules, 25(10), Article 2422. https://doi.org/10.3390/molecules25102422

Misra, A., Chaudhary, M. K., Tripathi, D., Srivastava, P. K., Gupta, V., Acharya, R., & Srivastava, S. (2023). Nutritional potential of an edible terrestrial orchid Eulophia nuda LINDL and validation of its traditional claim in arthritis. Journal of Ethnopharmacology, 306, Article 116123. https://doi.org/10.1016/j.jep.2022.116123

Natta, S., Mondol, M. S. A., Pal, K., Mandal, S., Sahana, N., Pal, R., Pandit, G. K., Alam, B. K., Das, S. S., Biswas, S. S., & NS, K. (2022). Chemical composition, antioxidant activity and bioactive constituents of six native endangered medicinal orchid species from north-eastern Himalayan region of India. South African Journal of Botany, 150, 248-259. https://doi.org/10.1016/j.sajb.2022.07.020

Nguyen, H. C., Lin, K.-H., Huang, M. Y., Yang, C. M., Shih, T. H., Hsiung, T.-C., Lin, Y.-C., & Tsao, F.-C. (2018). Antioxidant activities of the methanol extracts of various parts of Phalaenopsis orchids with white, yellow, and purple flowers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 457-465. https://doi.org/10.15835/nbha46211038

Obsuwan, K., Ryong, J. B., & Maksup, S. (2019). Analysis of bioactive compounds, polysaccharides and antioxidant activity in different parts of Dendrobium “Sonia Jo Daeng.” Science, Engineering and Health Studies, 13(2), 73-82.

Padhee, S., Mohanty, D., Kerry, R. G., Das, P. K., Jena, S., Sahoo, A., Panda, P. C., Mohanty, S., Ray, A., Khan, H. A., & Nayak, S. (2025). Ultra‐performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based profiling and evaluation of antioxidant, antityrosinase, antimicrobial and antiproliferative activities of Eulophia nuda Lindl.: A medicinal orchid. Chemistry and Biodiversity, 22(8), Article e202402833. https://doi.org/10.1002/cbdv.202402833

Parveen, S., Ramesh, C. K., Mahmood, R., & Pallavi, M. (2018). Folklore medicinal orchids from south india: The potential source of antioxidants. Asian Journal of Pharmaceutical and Clinical Research, 11(6), 194-198. https://doi.org/10.22159/ajpcr.2018.v11i6.24726

Pathak, P., Kumari, A., Chandler, B. D., & Zettler, L. W. (2023). In vitro propagation and phytochemical analysis of Vanda cristata Wall. ex Lindl: An endangered medicinal orchid of biopharmaceutical importance. South African Journal of Botany, 153, 109-123. https://doi.org/10.1016/j.sajb.2022.11.023

Paudel, M. R., Chand, M. B., Pant, B., & Pant, B. (2018). Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS. BMC Complementary and Alternative Medicine, 18(1), Article 134. https://doi.org/10.1186/s12906-018-2197-6

Paudel, M. R., Chand, M. B., Pant, B., & Pant, B. (2019). Assessment of antioxidant and cytotoxic activities of extracts of Dendrobium crepidatum. Biomolecules, 9(9), Article 478. https://doi.org/10.3390/biom9090478

Perkins, J., Hayashi, T., Peakall, R., Flematti, G. R., & Bohman, B. (2023). The volatile chemistry of orchid pollination. Natural Product Reports, 40(4), 819-839. https://doi.org/10.1039/d2np00060a

Rahamtulla, M., Mallikarjuna, K., & Khasim, S. M. (2023). GC-MS analysis and therapeutic importance of leaf extracts of Dendrobium aphyllum (Roxb.) C.E.C. Fischer: An in vitro study. South African Journal of Botany, 153, 62-76. https://doi.org/10.1016/j.sajb.2022.12.011

Rahman, M., Surag, A. T., Begum, R., Tusher, M. S. H., & Huda, M. K. (2022). Phytochemical, antioxidant, anti-inflammatory, and thrombolytic properties of Cleisomeria lanatum (Lindl.) Lindl. ex G. Don. Scientifica, 2022(1), Article 5660527 https://doi.org/10.1155/2022/5660527

Ramya, M., Jang, S., An, H.-R., Lee, S.-Y., Park, P.-M., & Park, P. H. (2020). Volatile organic compounds from orchids: From synthesis and function to gene regulation. International Journal of Molecular Sciences, 21(3), Article 1160. https://doi.org/10.3390/ijms21031160

Roy, A., Khan, A., Ahmad, I., Alghamdi, S., Rajab, B. S., Babalghith, A. O., Alshahrani, M. Y., Islam, S., & Islam, M. R. (2022). Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. BioMed Research International, 2022(1), Article 5445291. https://doi.org/10.1155/2022/5445291

Sein, K. L., Lertnitikul, N., Suttisri, R., & Jianmongkol, S. (2023). Anticancer and chemosensitizing activities of stilbenoids from three orchid species. Naunyn-Schmiedeberg’s Archives of Pharmacology, 396(4), 749-758. https://doi.org/10.1007/s00210-022-02352-x

Shengji, P., & Zhiwei, Y. (2018). Orchids and its uses in chinese Chinese medicine and health care products. Medical Research and Innovations, 2(1), 1-3. https://doi.org/10.15761/mri.1000133

Shukla, M. K., Monika, Thakur, A., Verma, R., Lalhlenmawia, H., Bhattacharyya, S., Bisht, D., Singh, A., Parcha, V., & Kumar, D. (2022). Unravelling the therapeutic potential of orchid plant against cancer. South African Journal of Botany, 150, 69-79. https://doi.org/10.1016/j.sajb.2022.07.005

Sukumaran, N. P., & Yadav, R. H. (2016). General unknown screening, antioxidant and anti-inflammatory potential of Dendrobium macrostachyum Lindl. Ancient Science of Life, 35(4), 240-244. https://doi.org/10.4103/0257-7941.188181

Suphrom, N., Pipatsawasdikul, K., Kongbangkerd, A., Chikun, K., Ngobkhonburi, S., Muaklek, B., Pitsamai, W., Nisaipham, B., Chuaimueang, W., Sasiri, P., Khongwet, S., Tothong, C., Pankaew, C., Insumrong, K., & Limmongkon, A. (2024). Bioactivity assay of Arundina graminifolia (D.Don) Hochr. extracts from diverse plant parts in Thailand: An assay-based investigation. Scientia Horticulturae, 327, Article 112876. https://doi.org/10.1016/j.scienta.2024.112876

Swainson, N. M., Pengoan, T., Khonsap, R., Meksangsee, P., Hagn, G., Gerner, C., & Aramrak, A. (2023). In vitro inhibitory effects on free radicals, pigmentation, and skin cancer cell proliferation from Dendrobium hybrid extract: A new plant source of active compounds. Heliyon, 9(9), Article e20197. https://doi.org/10.1016/j.heliyon.2023.e20197

Wati, R. K., Astuti, I. P., & Cahyaningsih, R. (2023). Inventorying medicinal orchid in Indonesia from global database. E3S Web of Conferences, 373, Article 05009. https://doi.org/10.1051/e3sconf/202337305009

Zhang, G.-Q., Liu, K.-W., Li, Z., Lohaus, R., Hsiao, Y.-Y., Niu, S.-C., Wang, J.-Y., Lin, Y.-C., Xu, Q., Chen, L.-J., Yoshida, K., Fujiwara, S., Wang, Z.-W., Zhang, Y.-Q., Mitsuda, N., Wang, M., Liu, G.-H., Pecoraro, L., Huang, H.-X., Xiao, X.-J., … Liu, Z.-J. (2017). The Apostasia genome and the evolution of orchids. Nature, 549(7672), 379-383. https://doi.org/10.1038/nature23897

Zhou, Z., Ying, Z., Wu, Z., Yang, Y., Fu, S., Xu, W., Yao, L., Zeng, A., Huang, J., Lan, S., Wang, X., & Liu, Z. (2021). Anthocyanin genes involved in the flower coloration mechanisms of Cymbidium kanran. Frontiers in Plant Science, 12, Article 737815. https://doi.org/10.3389/fpls.2021.737815