18 kDa PROTEIN ACCUMULATION IN SUGARCANE LEAVES UNDER DROUGHT STRESS CONDITIONS
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Abstract
The alteration of protein synthesis or degradation is one of the fundamental metabolic processes that affects drought tolerance. To investigate the protein profiles of sugarcane exposed to drought stress, two unrelated lines of sugarcane (Saccharum officinarum L.) Khon Kaen 1 and K86- 161were subjected to progressive water stress for 20 days. Under progressive drought stress condition, the Khon Kaen 1 line is sensitive to water stress. The modification of leaves were observed 2 weeks after the onset of water deficit. The Khon Kaen 1 leaves gradually turned yellow and wilted whereas the K86-161 leaves from the tolerant line remained green. The relative water content (RWC) in Khon Kaen 1 was decreased to 60% on day 20th whereas the RWC of K86-161 was slightly decreased and remained constant at 86% upon exposure to the water stress. In addition, when the protein changed in leaves were studied by two-dimensional electrophoresis, the accumulation of a 18 kDa protein was detected in drought-tolerant, K86-161 line. Antiserum raised against this protein, purified from SDS-PAGE, was used as a probe to detect the protein in sugarcane leaves by Western blotting technique. The titer of antigen-antibody interaction, was estimated to be 1:100. Furthermore, this polyclonal antibody was used to detect the accumulation of the 18 kDa protein in K86-161 and Khon Kaen 1 sugarcane leaves by Western blotting technique. The results show that the 18 kDa protein band from K86-161 expressed higher intensity than that of Khon Kaen 1 in equivalent to total protein amount. These results indicate that 18 kDa protein expressions had a high potential for development as a marker in any screening technique for drought-tolerant sugarcane cultivars.
Keywords: sugarcane, drought tolerance, polyclonal antibody, western blotting technique
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References
[2] Sugiharto, B. 2004 Biochemical and Molecular Studies on Sucrose-phosphate Synthase and Drought Inducible-protein in Sugarcane (Saccharum officinarum), Journal ILMU DASAR, 5(1), 62-67.
[3] Inman-Bamber, N.G. and Smith, D.M. 2005 Water Relations in Sugarcane and Response to Water Deficits, Field Crops Research, 92, 185-202.
[4] Yancey, P., Clark, M., Hand, S., Bowlus, R. and Somero, G. 1982 Living with Water Stress: Evolution of Osmolyte Systens, Science, 217, 1214-1222.
[5] Hare, P.D., Cress, W.A. and Staden, J. 1998 Dissecting the Roles of Osmolyte Accumulation During Stress, Plant, Cell and Environment, 21, 535-553.
[6] Zhang, S.Z., Yang, B.P., Feng, C. L. and Tang, H. L. 2005 Genetic Transformation of Tobacco with the Trehalose Synthase Gene from Grifola frondosa Fr. Enhances the Resistance to Drought and Salt in Tobacco, Journal of Integrative Plant Biology, 47(5), 579-587.
[7] Rhodes, D. and Hanson, A. 1993 Quaternary Ammonium and Tertiary Sulfonium Compounds in Higher Plants, Annual Review of Plant Physiology and Plant Molecular Biology, 44, 357-384.
[8] Ingram, J. and Bartels, D. 1996 The Molecular Basis of Dehydration Tolerance in Plants, ,Annual Review of Plant Physiology and Plant Molecular Biology, 47, 377-403.
[9] Reviron, M.P., Vartanian, N., Sallantin, M., Huet, J. C., Pernollet, J. C., and de Vienne, D. 1992 Characterization of A Novel Protein Induced by Progressive or Rapid Drought and Salinity in Brassica napus leaves, Plant Physiology, 100 (3), 1486-1493.
[10] Hogarth, D. M. 1987 Genetics of Sugarcane. In: Heinz, D. J., Ed. Sugarcane Improvement Through Breeding. Amsterdam, Elsevier, pp. 255-271.
[11] Sreenivasan, T. V., Ahooliwalia, B. S. and Heinz, D. J. 1987 Cytogenetics. In: Heinz, D. J., Ed. Sugarcane Improvement Through Breeding. Amsterdam, Elsevier, pp. 211-253.
[12] Ramagopal, S. 1990 Protein Polymorphism in Sugarcane Revealed by Two-dimensional gel Analysis. Theoretical and Applied Genetics, 79, 297-304.
[13] Lopez, C.G., Banowetz, G.M., Peterson, C.J. and Kronstad, W.E. 2003 Dehydrin Expression and Drough Tolerance in Seven Wheat Cultivars, Crop Science, 43, 577-582.
[14] Jiang, Y. and Huang, B. 2002 Protein Alterations in Tall Fescue in Response to Drought Stress and Abscisic Acid, Crop Science, 42, 202-207.
[15] Lopez, C.G., Banowetz, G.M., Peterson, C.J. and Kronstad, W.E. 2001 Differential Accumulation of A 24-kd Dehydrin Protein in Wheat Seedings Correlates with Drought Stress Tolerance at Grain Filling, Hereditas, 135, 175-181.
[16] Zang, X. and Komatsu, S. 2007 A Proteomics Approach for Identifying Osmotic-stress-Related Proteins in Rice, Phytochemistry, 68(4), 426-437.
[17] Riccardi, F., Gazeau, P., de Vienne, D. and Zivy, M. 1998 Protein Changes in Response to Progressive Water Deficit in Maize. Quantitative Variation and Polypeptide Identification, Plant Physiology, 117 (4), 1253-1263.
[18] Salekdeh, G.H., Siopongco, J., Wade, L.J., Ghareyazie, B. and Bennett, J. 2002 A Proteomics Approach to Analyzing Drought and Salt-responsiveness in Rice, Field Crops Research, 76, 199-219.
[19] Laemmli, U.K. 1970 Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, 227(5259), 680-685.
[20] O’Farrell, P.H. 1975 High Resolution Two-dimensional Electrophoresis of Proteins, The Journal of Biological Chemistry, 250(10), 4007-4021.
[21] Havaux, M., Canaani, O. and Malkin, S. 1986 Photosynthetic Responses of Leaves to Water Stress, Expressed by Photoacoustics and Related Methods: I. Probing the Photoacoustic Method as An Indicator for Water Stress in Vivo, Plant Physiology, 82(3), 827-833.
[22] Arora, R., Pitchay, D.S. and Bearce, B.C. 1998 Water-stress-induced Heat Tolerance in Geranium Leaf Tissue: A Possible Linkage Through Stress Proteins? Physiologia Plantarum, 103, 24-34.
[23] Bewley, J.D., Larsen, K.M. and Papp, J.E.T. 1983 Water-stress Induced Changes in the Pattern of Protein Synthesis in Maize Seeding Mesocotyls: A Comparison with the Effects of Heat Shock, Journal of Experimental Botany, 34, 1126-1133.
[24] Perez-Molphe-Balch, E., Gidekel, M., Segura-Nieto, M., Herrera-Estrella, L. and Ochoa-Alejo, N. 1996 Effects of Water Stress on Plant Growth and Root Proteins in Three Cultivars of Rice (Oryza sativa) with Different Level of Drought Tolerance, Physiologia Plantarum, 96, 284-290.
[25] Wu, G., Robertson, A.J., Zheng, P., Liu, X. and Gusta, L.V. 2005 Identification and Immunogold Localization of a Novel Bromegrass (Bromus inermis Leyss) Peroxisome Channel Protein Induced by ABA, Cold and Drought Stresses, and Late Embryogenesis, Gene, 363, 77-84.