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Cyprinid herpesvirus 3 (CyHV-3) or Koi herpesvirus (KHV) is a virulence viral infection in common carp and koi. The disease has caused global epizootic and economic loss in fish aquaculture and in the wild. Clinacanthus nutans (Burm. f.), belonging to the family Acantaceae, is a well-known medicinal plant used in Thai traditional medicine. Virucidal effects of the plant extract against human herpes simplex virus have been reported. In this study, C. nutans crude extract, dichloromethane fraction and n-butanol fraction were tested for antiviral activities against CyHV-3 in koi fin cell line. The extracts were tested for cytotoxicity, pre and post infection antiviral activities. The 50% cytotoxic concentration of crude extract was at 1,701.57 µg/ml, dichloromethane fraction at 522.47 µg/ml and n-butanol fraction at 1,797.98 µg/ml. Effective concentrations of the crude extract pre and post-infection were at 250, 500 and 1,000 µg/ml. The dichloromethane fraction and n-butanol fraction did not have antiviral activity. The results showed that crude extract expressed antiviral activity against CyHV-3 which can be applied as therapeutic agents in common carp and koi in the future.
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