Determination of Cyanide in Cattle Whole Blood by Microdiffusion Spectrophotometric Method

Main Article Content

Sontana Mimapan
Anusorn Yooyen
Benjama Metharatanukul
Sawittree Inudom

Abstract

Cyanide is a chemical substance found in plants, pesticides, rodenticides and salts used in the industrial process such as stainless, refining precious metals (gold and silver). Cyanogenic glycoside, one of the chemical forms of cyanides, is naturally presented in many plants including cassava, sorghum and mimosa. Consuming toxic forage is the main cause of acute cyanide poisoning in livestock. In this study, we developed microdiffusion spectrophotometric method to validate the quantitative determination of cyanide in cattle whole blood. Microdiffusion extraction method was performed and the time required to achieve full recovery in the extraction was studied. The optimal time then was used to validate the quantitative determination of cyanide using UV-Vis spectrophotometer at 587 nm wavelength. It was found that the time required to achieve full recovery in the microdiffusion extraction was considerably 2 hours. The established method gave a linearity in a concentration ranging from 0.2 to 4.0 µg/ml. (r=0.997). The limit of detection (LOD) was 0.2 µg/ml while the limit of quantitation (LOQ) was 0.5 µg/ml (102 – 109%Recovery, 2.56 %RSD). The accuracy was evaluated using fortified sample blanks (0.5, 1.0 and 3.0 µg/ml) was 105.16, 97.24 and 92.43 µg/ml, respectively which ranged from 90-108% (standard at 80-120%) and the precision was evaluated by %RSD ranging from 1.73-2.56 (standard at <10) and HorRat ranging from 0.16-0.26 (standard at <2) which all are acceptable.  The method was then used for the determination of cyanide in 48 whole blood samples of cassava pulp-fed dairy cattle. The results revealed that the concentrations of cyanide ranged from 0.24 to 0.54 µg/ml, which were in the same range of normal expectation in blood of most animal species. In conclusion, the microdiffusion spectrophotometric method developed in this study provided accuracy and precision as well as proved to be easy and reliable and it should be suitable for use in blood of other species.

Article Details

How to Cite
Mimapan, S., Yooyen, A., Metharatanukul, B. and Inudom, S. (2018) “Determination of Cyanide in Cattle Whole Blood by Microdiffusion Spectrophotometric Method”, Journal of Mahanakorn Veterinary Medicine, 11(2), pp. 125–139. Available at: https://li01.tci-thaijo.org/index.php/jmvm/article/view/133919 (Accessed: 22 December 2024).
Section
Research Article

References

ตติย สีหร่าย. 2547. เอกสารประกอบการอบรม-สัมมนาวิชาการด้านอุตสาหกรรมอาหาร: การตรวจพิสูจน์ความถูกต้องของวิธีทดสอบทางเคมี. สถาบันอาหาร. กรุงเทพฯ. หน้า 1-44.
ทิพวรรณ นิ่งน้อย. 2549. แนวปฏิบัติการทดสอบความถูกต้องของวิธีวิเคราะห์ทางเคมี โดยห้องปฏิบัติการเดียว. กรมวิทยาศาสตร์ การแพทย์ กระทรวงสาธารณสุข. 124 หน้า.
ปานทิพย์ ศิริโชติ. 2551. เหตุการณ์กรณีผู้ประสบเหตุหมดสติและเสียชีวิตในโรงงานหน่อไม้ดอง. การสัมมนาเครือข่ายนิติพิษวิทยาแห่งประเทศไทย ครั้งที่2 ณ กรีนเลครีสอร์ท อ. เมือง จ.เชียงใหม่. [Online]. Available: http:// www.med.cmu.ac.th/dept/forensic/2012/images/files/forensic-toxicology 2560.pdf, Accessed August 31, 2016.
มาลินี ลิ้มโภคา. 2523. พิษวิทยาและการวินิจฉัยโรคทางสัตวแพทย์. พิมพ์ครั้งที่ 1. โรงพิมพ์จรัญสนิทวงศ์, กรุงเทพฯ. 276 หน้า.
มาลินี ลิ้มโภคา สุชาติ ศราธพันธุ์ วิมล จิระธนวัฒน์ วิมลพร จิระวัฒนพงศ์ นวรัตน์ สาครรัตน์ นิยมศักดิ์ อุปทุม และ ชาญยุทธ เทพา. 2525. ไมยราบไร้หนามเป็นพิษในโค. กรุงเทพฯ: วารสารสัตวแพทย์. 3(1): 43-48.
มาลินี ลิ้มโภคา. 2527. พิษวิทยาและปัญหาที่พบในสัตว์. โรงพิมพ์จรัญสนิทวงศ์ กรุงเทพฯ. หน้า 364.
ศิริรัตน์ บัวผัน. 2555. เทคนิคการใช้กากมันสดเป็นอาหารโคนม. วารสารปศุสัตว์เกษตรศาสตร์. ปีที่38. ฉบับที่ 151. หน้า 48-50.
Alex, P.C., Rajankutty, K., Valsala, K.V. and Nair, K.N.M. 1991. Mimosa poisoning in a heifer. J. Vet. Anim. Sci. 22(1): 134-136.
Andre, T.G., Isis, M.H., Helena, M., Viviane, M.M., Paulo, C.M. and Silvana, L.G. 2015. Intoxication by cyanide in pregnant sows: prenatal and postnatal evaluation. J. Toxicol. 2015: 1-9.
Arnold, M., Gaskill, C., Smith, R. and Lacefield, G. 2014. Cyanide poisoning in ruminants. University of Kentucky College of Agriculture, Food and Environment. p. 1-2. [Online]. Available: http://www2.ca.uky.edu/agcomm/pubs/ID/ID220/ID220.pdf. Accessed August 30, 2016.
Ballantyne, B. 1987. Toxicology of cyanides. In: Ballantyne, B., Marrs, T.C., editors. Clinical and Experimental Toxicology of Cyanides. Bristol, UK: Wrights. p. 41-126.
Ballantyne, B. and Mars, T.C. 1987. Clinical and Experimental Toxicology of Cyanides. Bristol, UK, Wright.
Bourke, C.A., Bunker, E.C., Reece, R.L. and Whittaker, S.J. 2008. Cerebella ataxia in sheep grazing pastures infested with Romulea rosea (onion grass or Guildford grass). Aust. Vet. J. 86(9): 354-6.
Bradley, G.A., Metcalf, H.C., Reggiardo, C., Noon, T.H., Bicknell, E.J., Lozano-Alarcon, F., Reed, R.E. and Riggs, M.W. 1995. Neuroaxonal degeneration in sheep grazing Sorghum pastures. J. Vet. Diag. Invest. 7: 229-336.
Boadas-Vaello, P., Jover, E., Llorens, J. and Bayona, J.M. 2008. Determination of cyanide and volatile alkylnitriles in whole blood by headspace solid-phase microextraction and gas chromatography with nitrogen phosphorus detection. J Chromatogr. B Analyt. Technol. Biomed. Life Sci. 870(1): 17-21.
Chinaka, S., Takayama, N., Michigima, Y., and Ueda, K. 1998. Simultaneous determination of cyanide and thiocyanate in blood by ion chromatography with fluorescence and ultraviolet detection. J. Chromatogr. B Biomed. Sci. Appl. 713: 353–359.
Flanagan, R.J., Braithwaite, R.A., Brown, S.S., Widdop, B. and de Wolff, F.A. 1995. Cyanide. In: Basic Analytical Toxicology. World Health Organization. Geneva. p. 117-121.
Holzbecher, M. and Ellenberger, H.A. 1985. An evaluation and modification of a microdiffusion method for the emergency determination of blood cyanide. J. Anal. Toxicol. 9: 251 – 253.
Horwitz, W. and Albert, R. 2006. The Horwitz Ratio (HorRat): A useful index of method performance with respect to precision. J. AOAC Inter. 89: 1095–1109.
Lerdsri, J., Doonsungnern, A. and Chuachan, U. 2014. Nitrate poisoning due to ingestion of Mimosa invisa in cattle. Proceedings of the 3rd Thailand-Japan joint conference on animal health. p. 41.
Lundquist, P., Kagedal, B. and Nilsson, L. 1995. An improved method for determination of thiocyanate in plasma and urine. Eur. J. Clin. Chem. Clin. Biochem. 33: 343–349.
Manzano, H., de Sousa A.B., Soto-Blanco B., Guerra J.L., Maiorka P.C. and Gorniak S.L. 2007. Effects of long-term cyanide ingestion by pigs. Vet. Res. Commu. 31(1): 93-104.
Mateus, F.H., Lepera, J.S. and Lanchote, V.L. 2005. Determination of acetonitrile and cyanide in rat blood: Application to an experimental study. J. Anal. Toxicol. 29(2): 105–109.
McKenzie, R.A. and McMicking, L.I. 1977. Ataxia and urinary incontinence in cattle grazing sorghum. Aust. Vet. J. 53: 496-497.
Morgan, R.L. and Way, J.L. 1980. Fluorometric determination of cyanide in biological fluids and pyridoxal. J. Anal. Toxicol. 4(2): 78-81.
Murphy, K.E., Schantz, M.M., Butler, T.A., Benner, B.A., Wood, L.J. and Turk, G.C. 2006. Determination of cyanide in blood by isotope-dilution gas chromatography-mass spectrometry. Clin. Chem. 52: 458-467.
Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. 2007. Veterinary Medicine; A text book of the disease of cattle, horses, sheep, pigs and goats. 10th ed. Saunders Publishers, London, UK. p. 1852-1854.
Rajan, A., Manomohan, C.B., Valsala, K.V., Sreekumaran, K.M. and Nair, N.D. 1986. Experimental studies on the toxicity of the plant Mimosa invisa in calves. Kelara J. Sci. 17: 91-98.
Shafi, H., Subhani, A., Imran, M., Watoo, S.A., Sarwar, M., Hussan, S.S., Latif, A., Ashiq, M.Z., Tahir, M.A. and Tahir, A.M. 2015. Determination of cyanide in biological and non-biological matrices by headspace gas chromatography coupled to flame - ionization detector. Arab J. Foren. Sci. Foren. Med. 1(1): 123–129.
Soto - Blanco, B., Gorniak, S.L. and Kimura, E.T. 2001. Physiopathological effects of the administration of chronic cyanide to growing goats- a model for ingestion of cyanogenic plants. Vet. Res. Commun. 25(5): 379-389.
The Merck Veterinary Manual. 2014. “Overview of Cyanide Poisoning”. [Online]. Available : http://www.merck vetmanual.com/mvm/toxicology/cyanide_poisoning/overview_of_cyanide_poisoning.html. Accessed February 12, 2016.
Tsuge, K., Kataoka, M. and Seto, Y. 2001. Rapid determination of cyanide and azide in beverages by microdiffusion spectrophoto metric method. J. Anal. Toxicol. 25: 228–236.
Usha, P.T.A., Gopakumar, N. and Chandrasekharan, A.M.N. 2009. Toxicity of fresh juice of Mimosa invisa in rabbits. J. Vet. Anim. Sci. 40: 6-8.
Way, J.L. 1984. Cyanide intoxication and its mechanism of antagonism. Annu. Rev. Pharmacol. Toxicol. 24: 451-481.