Effects of Colchicine Concentration Level and Soaking Period to Induce Polyploid Mutation on Five Forage Sorghum (Sorghum bicolor L. Moench) Cultivars

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Worames Poosamart
Naris Siniri
Sakunkan Simia


This study was to investigate the effect of the colchicine concentration level, soaking period, and five forage sorghum (Sorghum bicolor L. Moench) cultivars on the occurrence of polyploidy. The 4x5x5 factorial arrangement in Completely Randomized Design (CRD) was employed through 3 factors: 4 colchicine concentrations level (0, 0.1, 0.2 and 0.3%), 5 soaking periods (0, 12, 24, 48 and 72 hours) and 5 forage sorghum cultivars (Cowley, Rio, IS23585, Keller and Wray). The results revealed that there was a significant difference among colchicine concentrations level, soaking periods, and sorghum cultivars; when colchicine concentration level increased and soaking period increased, the survival rate of sorghum decreased variously but occurrence of polyploidy chromosome increased differently among cultivars. Cowley sorghum soaking in the colchicine concentration at 0.1% for both 48 and 72 hours had mutation polyploidy 3.3 and 2.3 % respectively. Cowley sorghum soaking in the colchicine concentration at 0.2% for both 24 and 48 hours had mutation polyploidy 4.1 and 3.2 % respectively and soaking in the colchicine concentration at 0.3% for 24 hours had mutation polyploidy 4.0%. While, Rio sorghum soaking in the colchicine concentration at 0.1% for 48 hours had mutation polyploidy 2.2 % and soaking in the colchicine concentration at 0.2 % for 24 and 48 hours had mutation polyploidy 5.2 and 2.6 % respectively. For IS23585, Keller and Wray sorghum cultivars weren't significant different effect of the colchicine concentration level and soaking period. However, considering the frequency of the occurrence of seedling having the polyploidy chromosome soaking in the colchicine concentration of 0.2% for 48 hours was the most appropriate concentration and period or duration for the occurrence of the plant with the polyploidy chromosome.


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Chainat Research and Development of Animal Feeds. 2015. (cited 8 June 2015) Introduction to Animal Feeds Plants. Available from: http://nccn-cnt.dld.go.th/th/index.php?option=com_content&view=article&id=108:-sweet-sorghum-keller&catid=53:2011-11-27-05-4 2-43&Itemid=55.
Chaiyasut, K. 1989. Cytogenetics and Plant Taxonomy of Zephyranthes. Indian Store publishing. Bangkok. 260 p.
Chen, Q.F., Wang, C.L., Lu, Y.M., AfzaShen, M.r. Duren, M.V and Brunner, H. 2001. Anther culture in connection with induced mutations for rice improvement in vitro induction of tetraploidinpomegrate (Punicagranatum). Euphytica. 120: 401-408.
Daker, M.G. 1967. Cytological studies on a haploid cultivar of pelargonium, and its colchicine- induced diploids. ChromosomaBerl. 21: 250-271.
Dhooghe, E., Van-Laere, E.K., Eeckhaut, T., Leus, L. and Huylenbroeck, J. Van. 2011. Mitotic chromosome doubling of plant tissues in vitro. Plat Cell Tiss Organ Cult 104: 359-373.
FAO. 2008. (cited 8 June 2015) Sorghum bicolor (L.) Moench. Available from: http://www.fao.org/ag/AGP/AGPC/doc/Gbase/data/Pf00031 9.HTM.
Ghaffari, S.M. 2006. Occurrence of diploid and polyploid microspores in Sorghum bicolor (Poaceae) is the result of cytomixis. African Journal of Biotechnology. 5(16): 1450-1453.
Glowacka, K., Jezowski, S. and Kaczmarek, Z. 2010. In vitro induction of polyploidy by colchicine treatment of shoots and preliminary characterization of induced polyploids in two Miscanthus species. Industrial crops and Products. 32: 88-96.
Gu, X.F., Yang, A.F., Meng, H. and Zhang, J.R. 2005. In vitro induction of tetrapolid plants from diploid Zizyphus jujube Mill. cv. Zhanhua. Plant Cell Report. 24: 671-676.
Jonathan, M.L., Bran, M.H. and Lubell, J.D. 2008. Induction of tetraploidy in meristematically active seeds of Japanese barberry (Berberisthunbergii var. atropurpurea) through exposure to colchicines and oryzalin. ScientiaHorticulturea 119: 67-71.
Khacharoen, S. 1999. Feeds and Feeding for Ruminants. KhonKaen University publishing. KhonKaen. 685.
Lamseejan, S., Jompuk, P., Wongpiyasatid, A., Kwanthammachart, P. and. Meesat, R. 2001. Improvement of ornamental plants through induced mutations IAEA-SR-210/11 Working Material Mutation Techniques and Molecular Genetics for Tropical and Subtropical Plant Improvement in Asia and the Pacific Region. Report of an FAO/IAEA Seminar, held in Makaticity, The Philippines, 11-15 October 1999. Reproduced by the IAEA, Vienna, Austria, 2001. p. 19-20.
Lertprasertrat, K., Jatupornpong, S. and Oeamsuphasit, N. 1992. Variation in sorghum populations GPT 7R and GPTM 3BR and breeding used. Proceedings of the 30th Kasetsart University Annual Conference: Plants. Press. 29 January- 1 February Kasetsart Univ. Bangkok. p: 513-518.
Levine, M. 1945. Colchicine and X-Ray in the treatment of plant and animal overgrowths. The Botanical Review. XI(3): unpaged.
Luca, C. 2000. Genetic and epigenetic interaction in allopolyploid plant. Plant Molecular Biology 43: 387-399.
Maiti, R.K. 1996. Sorghum Science. Science Publishers. New Delhi, India. p 352.
Mujib, A.C. 2005.Induced Morphological Variants in Pineapple.Plant Tissue Cult.& Biotech. 15(2): 127-133.
Newcomer, E.H. 1941. A colchicine- induced homozygous tomato obtained through doubling clonal haploid. Proc. Amer. Soc. Hort. Sci. 38: 610-628.
Pholsen, S., Higgs, D.E.B. and Suksri, A.. 2001. Effects of nitrogen and potassium fertilisers on growth, chemical components, and seed yields of forage sorghum (Sorghum bicolor L. Moench) grown on OxicPaleustults soil, Northeast Thailand. Pakistan J. Bio. Sci. 4(1): 27-31.
Poosamart, W. 2008. Effect of planting dates on growth, dry weight yield, seed yield and fodder quality of main crop and ratoon crop of IS23585 forage sorghum cultivar (Sorghum Bicolor L. Moench) growth on Karat soil series (Oxic paleustults). Thesis Master of Animal Science. Khon Kaen University. Khon Kaen. p. 75.
Rao, P.S. and Suprasanna, P. 1996. Method to double haploid chromosome numbers. In : Vitro Haploid Production in Higher Plants, S.M. Jain; S.K. Sopory and R.E. Velleux, Eds. vol. 1, Kluwer Academic Publishers, Dordrecht, The Netherlands, 1996. p 317–339.
Shao, J., Deng, C.C.X., Herrea, J.C., Moreno, L.G., Acuna, J.R., Pena, M.De. and Osorio, D. 2003. Colchicine-induced microspore embryo genecoffee. PlantCell, Tissue and Organ Culture. 75: 241-246.
Suksri, A. and Pholsen, S. 1999. Growth analysis of sorghum (Sorghum bicolor L. Moench) with respect to phosphorus and potassium levels on yield and fodder qualities grown on Yasothon soil series. unpaged. In: An Annual Report 1999. Faculty of Agriculture, KhonKaen University, KhonKaen, Thailand.
Wei, L.H., Dong-nan, L. and X-y.Hui, C. 2007. Polyploid induction of Lespedeza formosa by colchicine treatment. For.Study.China. 9(4): 283-286.
Wu, J.H. and Mooney, P. 2004. Autotetraploidtangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine. Plant Cell. Tissue and Organ Culture 73: 35-41.
Xu, L., Najeeb, U. Naeem, M.S. Daud, M.K. Cao, J.S. Gong, H.J. and Zhou, W.J. 2010. Induction of tetraploidy in Juncuseffusus by colchicine. BiologiaPlantarum. 54(4): 659-663.
Yang, X.M., Cao, Z.Y., An, L.Z., Wang, Y.M. and Fang, X.W. 2006. In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitisvinifera L.). Euphytica. 152(2): 217-224.
Zhunsuwan, W. 2005. (cited 8 June 2015) Sorghum, Great millet, Guinea corn, Kafir corn, Mtamajuwar, Cholamkaoliang, Milo-maize, Sorghum bicolor (L.) Moench. www.natres. psu.ac.th/Department/.../sorghum.doc.