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The current study was aimed to evaluate effectiveness of anthelminthics; albendazole, ivermectin and levamisole in a breeding stock White Dorper sheep farm. Totally, 29 White Dorper sheep were included in this study and then allocated into 4 groups which were Control, Albendazole, Ivermectin and Levamisole for 6, 7, 8 and 8 animals, respectively. Dose (route of administration) of 10 (oral), 0.2 (subcutaneous) and 8 (subcutaneous) mg/kg BW was applied for the sheep in the group of Albendazole, Ivermectin and Levamisole, respectively. Fecal samples were collected individually from the experimental sheep at day 0, 7, 14, 28, 42 and 56 days after the start of the experiment. Quantity of helminth egg was determined by the McMaster technique and expressed as egg count per gram or EPG. Percentage of reduction of helminth eggs was calculated at different times after the start of the study. Fecal egg count reduction (FECR) was also calculated to determine anthelmintic resistance occurrence. The results showed that strongyle and strongyloides egg types found, with mainly strongyle egg type (86.21%) and an average of 3.55±0.11 log10 EPG+1 at the start of the study. After anthelmintic administration, levamisole significantly lowered (P<0.05) quantity of helminth eggs at day 7 (81.98% of reduction) and day 14 (90.19% of reduction) after anthelmintic administration. In the mean times, albendazole and ivermectin had effectiveness of 2.18% – 35.78% for helminth egg reduction at day 7 and 14 after anthelmintic administration. The value of FECR at 7 and 14 days after de-worming indicated anthelmintic resistance for albendazole and ivermectin, but gastro-intestinal helminths of the sheep were susceptible to levamisole with rather high effectiveness of levamisole (FECR = 99.92). Therefore, levamisole had high effectiveness for gastro-intestinal helminth control of sheep in the studied farm.
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Arsenopoulos, K.V., G.C. Fthenakis, E.I. Katsarou, and E. Papadopoulos. 2021. Haemonchosis: A challenging parasitic infection of sheep and goats. Animals. 11: 363.
Ballweber, L. R. 2001. Veterinary Parasitology (Practical veterinarian). Butterworth-Heinemann. United States of America. p.319.
Borges, S.L., A.A. Oliveira, L.R. Mendonça, S.M. Lambert, J.M. Viana, S.M. Nishi, F.S. Julião, and M.A.O Almeida. 2015. Anthelmintic resistance in goat herds in the Caatinga and Mata Atlântica biomes. Pesq. Vet. Bras. 35(7): 643-648.
Bosco, A., J. Kießler, A. Amadesi, M. Varady, B. Hinney, D. Ianniello, M.P. Maurelli, G. Cringoli, and L. Rinaldi. 2020. The threat of reduced efficacy of anthelmintics against gastrointestinal nematodes in sheep from an area considered anthelmintic resistance-free. Parasit. Vectors. 13(1): 1-12.
Chandrawathani, P., O. Jamnah, P.J. Waller, M. Larsen, and A.T. Gillespie. 2004. Field studies on the biological control of nematode parasites of sheep in the tropics, using the microfungus Duddingtonia flagrans. Vet Parasitol. 120: 177–187.
Coles, G.C., C. Bauer, F.H.M. Borgsteede, S. Geerts, T.R. Klei, M.A. Taylor, and P.J. Waller. 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 44: 35–44.
Crook, E.K., D.J. O’Brien, S.B. Howell, B.E. Storey, N.C. Whitley, J.M. Burke, and R.M. Kaplan. 2016. Prevalence of anthelmintic resistance on sheep and goat farms in the mid-Atlantic region and comparison of in vivo and in vitro detection methods. Small Rumin. Res. 143: 89-96.
Dorny, P., E. Claerebout, J. Vercruysse, R. Sani, and A. Jalila. 1994. Anthelmintic resistance in goats in peninsular Malaysia. Vet. Parasitol. 55: 327–342.
Falzon, L.C., P.I. Menzies, K.P. Shakya, A. Jones-Bitton, J. Vanleeuwen, J. Avula, H. Stewart, J.T. Jansen, M.A. Taylor, J. Learmount, and A.S. Peregrine. 2013. Anthelmintic resistance in sheep flocks in Ontario, Canada. Vet. Parasitol. 193(1-3): 150-162.
Falzon, L.C., J. van Leeuwen, P.I. Menzies, A. Jones-Bitton, W. Sears, J.T. Jansen, and A.S. Peregrine. 2015. Erratum to: Comparison of calculation methods used for the determination of anthelmintic resistance in sheep in a temperate continental climate. Parasitol. Res. 114(4): 1631-1643.
Geurden, T., C. Chartier, J. Fanke, A.F. Di Regalbono, D. Traversa, G. von Samson-Himmelstjerna, J. Demeler, H.B. Vanimisetti, D.J. Bartram, and M.J. Denwood. 2015. Anthelmintic resistance to ivermectin and moxidectin in gastrointestinal nematodes of cattle in Europe. Int. J. Parasitol. Drugs Drug Resist. 5(3): 163-171.
Glover, M., C. Clarke, L. Nabb, and J. Schmidt. 2017. Anthelmintic efficacy on sheep farms in south-west England. Vet. Rec. 180: 378.
Goolsby, M.K., M.L. Leite-Browning, and R. Browning. 2017. Evaluation of parasite resistance to commonly used commercial anthelmintics in meat goats on humid subtropical pasture. Small Rumin. Res. 146: 37-40.
Jacobs, D. E., M. Fox, L. Gibbons, and C. Hermosilla. 2016. Principles of Veterinary Parasitology. John Wiley & Sons, Ltd., United Kingdom. p.680.
Jaiswal, A.K., V. Sudan, D. Shanker, and P. Kumar. 2013. Emergence of ivermectin resistance in gastrointestinal nematodes of goats in a semi-organized farm of Mathura district-India. Vet. Archiv. 83: 275-280.
Limcharoen, S., M. Khongsen, and S. Srisawat. 2013. Herbs used for eradication of gastro-intestinal nematodes in goats. Princess Naradhiwas Univ. J. 5(4): 164-173.
Limcharoen, S., M. Khongsen, S. Taahai, and M. Peeyao. 2018. Effects of levamisole, ivermectin and albendazole on eradication of gastro - intestinal parasite in goats (Capra hircus). Khon Kaen Agr. J. 46(suppl.1): 622-627.
Mahieu, M., B. Ferre, M. Madassamy, and N. Mandonnet. 2014. Fifteen years later, anthelmintic resistances have dramatically spread over goat farms in Guadeloupe. Vet. Parasitol. 205(1-2): 379-384.
Maingi, N., R.C. Krecek, and N. van Biljon. 2006. Control of gastrointestinal nematodes in goats on pastures in South Africa using nematophagous fungi Duddingtonia flagrans and selective anthelmintic treatments. Vet. Parasitol. 138: 328–336.
Maqbool, I., Z.A. Wani, R.A. Shahardar, I.M. Allaie, and M.M. Shah. 2017. Integrated parasite management with special reference to gastro-intestinal nematodes. J. Parasit. Dis. 41(1): 1-8.
Mavrot, F., H. Hertzberg, and P. Torgerson. 2015. Effect of gastro-intestinal nematode infection on sheep performance: a systematic review and meta-analysis. Parasit. Vectors. 8: 557.
Myers, M.J., K.D. Howard, and J.C. Kawalek. 2021. Pharmacokinetic comparison of six anthelmintics in sheep, goats, and cattle. J. Vet. Pharmacol. Ther. 44(1): 58-67.
Paduang, S., and R. Thongtha. 2020. Study of anthelmintic resistance of gastrointestinal nematodes in goats in Ratchaburi province. J. Kasetsart Veterinarians. 30(1): 1-10.
Pipathsakulpak, M., and Pongthaisong, P. 2020. Epidemiology and risk factor of gastrointestinal anthelmintic resistance in goat. KKU Vet. J. 30(1): 33-41.
R Core Team. R: A Language and Environment for Statistical Computing. 2020. R Foundation for Statistical Computing, Vienna, Austria.
Richard, J., and P. Alan. 2017. A brief review on the mode of action of antinematodal drugs. Acta Vet. (Beogr). 67(2): 137–152.
Research Team in Canada. 2010. Handbook for the Control of Internal Parasites of Sheep. University of Guelph, Canada. p.56.
Research Team in Canada. 2019. Handbook for the Control of Internal Parasites of Sheep and Goat. University of Guelph, Canada. p.64.
Sangster, N.C., A. Cowling, and R.G. Woodgate. 2018. Ten events that defined anthelmintic resistance research. Trends Parasitol. 34: 553–563.
Sprenger, L.K., C.H. Amaral, R.V. Leite Filho, T.N. Aguiar, and M.B. Molento. 2013. Effectiveness of phosphate levamisole in gastrointestinal nematodes of goats and sheep. Arch. Vet. Sci. 18(1): 29-39.